Potential Pathways Involved in Elaidic Acid Induced Atherosclerosis in Human Umbilical Vein Endothelial Cells
Researches have demonstrated that trans-fatty acids are related to the progression of atherosclerosis, but the underlying mechanism is not clear till now. In the presented study, two-dimensional electrophoresis based proteomics was used to discover the role of elaidic acid in atherosclerosis. In hum...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2017/8932876 |
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| author | Huahong Yu Xiangmei Li Zhongshang Liang Bin Qiu Siguang Li Ting Luo Jing Li Hongyan Li Zeyuan Deng |
| author_facet | Huahong Yu Xiangmei Li Zhongshang Liang Bin Qiu Siguang Li Ting Luo Jing Li Hongyan Li Zeyuan Deng |
| author_sort | Huahong Yu |
| collection | DOAJ |
| description | Researches have demonstrated that trans-fatty acids are related to the progression of atherosclerosis, but the underlying mechanism is not clear till now. In the presented study, two-dimensional electrophoresis based proteomics was used to discover the role of elaidic acid in atherosclerosis. In human umbilical vein endothelial cells (HUVEC), twenty-two and twenty-three differentially expressed proteins were identified in low (50 μmol/L) and high (400 μmol/L) concentration elaidic acid simulated groups, respectively, comparing with the control group. The expressions of some selected proteins (PSME3, XRCC5, GSTP1, and GSTO1) were validated by qRT-PCR analysis. Western blotting analysis further confirmed that elaidic acid downregulated the expression of PSME3 and XRCC5. Moreover, P53, the downstream protein of PSME3, was further investigated. Results demonstrated that a variety of proteins, many of which were related to oxidative stress, apoptosis, and DNA damage, were involved in the elaidic acid induced atherosclerosis. Furthermore, P53 was demonstrated to regulate the atherosclerosis through cell cycle arrest and apoptosis pathway. |
| format | Article |
| id | doaj-art-d8c4636572024822b6d760a042197c2d |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-d8c4636572024822b6d760a042197c2d2025-02-03T05:49:36ZengWileyJournal of Chemistry2090-90632090-90712017-01-01201710.1155/2017/89328768932876Potential Pathways Involved in Elaidic Acid Induced Atherosclerosis in Human Umbilical Vein Endothelial CellsHuahong Yu0Xiangmei Li1Zhongshang Liang2Bin Qiu3Siguang Li4Ting Luo5Jing Li6Hongyan Li7Zeyuan Deng8State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi, ChinaInstitute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaStem Cell Translational Research Center, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi, ChinaResearches have demonstrated that trans-fatty acids are related to the progression of atherosclerosis, but the underlying mechanism is not clear till now. In the presented study, two-dimensional electrophoresis based proteomics was used to discover the role of elaidic acid in atherosclerosis. In human umbilical vein endothelial cells (HUVEC), twenty-two and twenty-three differentially expressed proteins were identified in low (50 μmol/L) and high (400 μmol/L) concentration elaidic acid simulated groups, respectively, comparing with the control group. The expressions of some selected proteins (PSME3, XRCC5, GSTP1, and GSTO1) were validated by qRT-PCR analysis. Western blotting analysis further confirmed that elaidic acid downregulated the expression of PSME3 and XRCC5. Moreover, P53, the downstream protein of PSME3, was further investigated. Results demonstrated that a variety of proteins, many of which were related to oxidative stress, apoptosis, and DNA damage, were involved in the elaidic acid induced atherosclerosis. Furthermore, P53 was demonstrated to regulate the atherosclerosis through cell cycle arrest and apoptosis pathway.http://dx.doi.org/10.1155/2017/8932876 |
| spellingShingle | Huahong Yu Xiangmei Li Zhongshang Liang Bin Qiu Siguang Li Ting Luo Jing Li Hongyan Li Zeyuan Deng Potential Pathways Involved in Elaidic Acid Induced Atherosclerosis in Human Umbilical Vein Endothelial Cells Journal of Chemistry |
| title | Potential Pathways Involved in Elaidic Acid Induced Atherosclerosis in Human Umbilical Vein Endothelial Cells |
| title_full | Potential Pathways Involved in Elaidic Acid Induced Atherosclerosis in Human Umbilical Vein Endothelial Cells |
| title_fullStr | Potential Pathways Involved in Elaidic Acid Induced Atherosclerosis in Human Umbilical Vein Endothelial Cells |
| title_full_unstemmed | Potential Pathways Involved in Elaidic Acid Induced Atherosclerosis in Human Umbilical Vein Endothelial Cells |
| title_short | Potential Pathways Involved in Elaidic Acid Induced Atherosclerosis in Human Umbilical Vein Endothelial Cells |
| title_sort | potential pathways involved in elaidic acid induced atherosclerosis in human umbilical vein endothelial cells |
| url | http://dx.doi.org/10.1155/2017/8932876 |
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