Potential of Olive Oil Extract (Olea europaea) For Affecting Lipid Profile, Lipid Oxidative and Fatty Liver on Hiperlipemic Rats (Rattus norvegicus)
Olive oil (Olea europaea) contains 55 to 83% oleic acid which is a single chain unsaturated fatty acid or mono-unsaturated fatty acid (MUFA), and 2% phenolic components in the form of hydroxytyrosol and tyrosol. This study aims to determine the potential of olive oil extract (Olea europaea) in reduc...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Brawijaya
2019-06-01
|
| Series: | Journal of Experimental Life Science |
| Subjects: | |
| Online Access: | https://jels.ub.ac.id/index.php/jels/article/view/333 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850134612474331136 |
|---|---|
| author | Annisa Hanifwati Agung Pramana Warih Marhendra Aulanni'am Aulanni'am |
| author_facet | Annisa Hanifwati Agung Pramana Warih Marhendra Aulanni'am Aulanni'am |
| author_sort | Annisa Hanifwati |
| collection | DOAJ |
| description | Olive oil (Olea europaea) contains 55 to 83% oleic acid which is a single chain unsaturated fatty acid or mono-unsaturated fatty acid (MUFA), and 2% phenolic components in the form of hydroxytyrosol and tyrosol. This study aims to determine the potential of olive oil extract (Olea europaea) in reducing cholesterol and malondialdehyde levels, along with inhibiting fatty liver development in hiperlipemic rats. Sixteen rats were divided into four groups, the first group was healthy control group, the second group was positive control group, received a high-fat diet containing 100 mg of cholesterol powder, 25 mg of cholic acid powder and 1 mL of quail egg yolk for 28 days. Third and fourth treatment groups were received for 28 days, plus 0.828 g (1 mL) and 1.656 g (2 mL) of olive oil extract daily for 14 days, respectively. The measurement of total cholesterol used cholesterol test strips based on oxidation enzyme reaction cholesterol esterase and cholesterol oxidase that produce hydrogen peroxidation, then analyzed with biosensor refractophotometry. Measurement of malondialdehyde used thiobarbituric acid (TBA) test. Histological observation of fatty liver was assessed using a NAS (Non-alcoholic fatty liver disease score). The results showed that 1.656 g (2 mL) of olive oil extract per day decreased total cholesterol level up to 44.41 %, malondialdehyde level up to 61.75%, and NAS score up to 50%, compared with positive control. It was concluded that olive oil extract was decreasing total cholesterol level, as an anti-oxidant and prevent fatty liver development. |
| format | Article |
| id | doaj-art-d8b068f53ea74a24b42a8cc32a4a2f79 |
| institution | OA Journals |
| issn | 2087-2852 2338-1655 |
| language | English |
| publishDate | 2019-06-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Experimental Life Science |
| spelling | doaj-art-d8b068f53ea74a24b42a8cc32a4a2f792025-08-20T02:31:40ZengUniversity of BrawijayaJournal of Experimental Life Science2087-28522338-16552019-06-0192133138https://doi.org/10.21776/ub.jels.2019.009.02.12Potential of Olive Oil Extract (Olea europaea) For Affecting Lipid Profile, Lipid Oxidative and Fatty Liver on Hiperlipemic Rats (Rattus norvegicus)Annisa Hanifwati0Agung Pramana Warih Marhendra1Aulanni'am Aulanni'am2Universitas BrawijayaUniversitas BrawijayaUniversitas BrawijayaOlive oil (Olea europaea) contains 55 to 83% oleic acid which is a single chain unsaturated fatty acid or mono-unsaturated fatty acid (MUFA), and 2% phenolic components in the form of hydroxytyrosol and tyrosol. This study aims to determine the potential of olive oil extract (Olea europaea) in reducing cholesterol and malondialdehyde levels, along with inhibiting fatty liver development in hiperlipemic rats. Sixteen rats were divided into four groups, the first group was healthy control group, the second group was positive control group, received a high-fat diet containing 100 mg of cholesterol powder, 25 mg of cholic acid powder and 1 mL of quail egg yolk for 28 days. Third and fourth treatment groups were received for 28 days, plus 0.828 g (1 mL) and 1.656 g (2 mL) of olive oil extract daily for 14 days, respectively. The measurement of total cholesterol used cholesterol test strips based on oxidation enzyme reaction cholesterol esterase and cholesterol oxidase that produce hydrogen peroxidation, then analyzed with biosensor refractophotometry. Measurement of malondialdehyde used thiobarbituric acid (TBA) test. Histological observation of fatty liver was assessed using a NAS (Non-alcoholic fatty liver disease score). The results showed that 1.656 g (2 mL) of olive oil extract per day decreased total cholesterol level up to 44.41 %, malondialdehyde level up to 61.75%, and NAS score up to 50%, compared with positive control. It was concluded that olive oil extract was decreasing total cholesterol level, as an anti-oxidant and prevent fatty liver development.https://jels.ub.ac.id/index.php/jels/article/view/333fatty liverhyperlipidemiamalondialdehydeolea europaea. |
| spellingShingle | Annisa Hanifwati Agung Pramana Warih Marhendra Aulanni'am Aulanni'am Potential of Olive Oil Extract (Olea europaea) For Affecting Lipid Profile, Lipid Oxidative and Fatty Liver on Hiperlipemic Rats (Rattus norvegicus) Journal of Experimental Life Science fatty liver hyperlipidemia malondialdehyde olea europaea. |
| title | Potential of Olive Oil Extract (Olea europaea) For Affecting Lipid Profile, Lipid Oxidative and Fatty Liver on Hiperlipemic Rats (Rattus norvegicus) |
| title_full | Potential of Olive Oil Extract (Olea europaea) For Affecting Lipid Profile, Lipid Oxidative and Fatty Liver on Hiperlipemic Rats (Rattus norvegicus) |
| title_fullStr | Potential of Olive Oil Extract (Olea europaea) For Affecting Lipid Profile, Lipid Oxidative and Fatty Liver on Hiperlipemic Rats (Rattus norvegicus) |
| title_full_unstemmed | Potential of Olive Oil Extract (Olea europaea) For Affecting Lipid Profile, Lipid Oxidative and Fatty Liver on Hiperlipemic Rats (Rattus norvegicus) |
| title_short | Potential of Olive Oil Extract (Olea europaea) For Affecting Lipid Profile, Lipid Oxidative and Fatty Liver on Hiperlipemic Rats (Rattus norvegicus) |
| title_sort | potential of olive oil extract olea europaea for affecting lipid profile lipid oxidative and fatty liver on hiperlipemic rats rattus norvegicus |
| topic | fatty liver hyperlipidemia malondialdehyde olea europaea. |
| url | https://jels.ub.ac.id/index.php/jels/article/view/333 |
| work_keys_str_mv | AT annisahanifwati potentialofoliveoilextractoleaeuropaeaforaffectinglipidprofilelipidoxidativeandfattyliveronhiperlipemicratsrattusnorvegicus AT agungpramanawarihmarhendra potentialofoliveoilextractoleaeuropaeaforaffectinglipidprofilelipidoxidativeandfattyliveronhiperlipemicratsrattusnorvegicus AT aulanniamaulanniam potentialofoliveoilextractoleaeuropaeaforaffectinglipidprofilelipidoxidativeandfattyliveronhiperlipemicratsrattusnorvegicus |