Wound Healing Effects of New Cream Formulations with Herbal Ingredients

<b>Aim</b>: To prepare two different kinds of wound care creams containing plant extracts and examine their effectiveness in comparison with a placebo cream and a commercial wound care cream, Madecassol<sup>®</sup>. <b>Methods</b>: The two cream formulations were...

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Main Authors: Derya Algül, Ertuğrul Kılıç, Ferda Özkan, Yasemin Yağan Uzuner
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Pharmaceutics
Subjects:
Online Access:https://www.mdpi.com/1999-4923/17/7/941
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author Derya Algül
Ertuğrul Kılıç
Ferda Özkan
Yasemin Yağan Uzuner
author_facet Derya Algül
Ertuğrul Kılıç
Ferda Özkan
Yasemin Yağan Uzuner
author_sort Derya Algül
collection DOAJ
description <b>Aim</b>: To prepare two different kinds of wound care creams containing plant extracts and examine their effectiveness in comparison with a placebo cream and a commercial wound care cream, Madecassol<sup>®</sup>. <b>Methods</b>: The two cream formulations were developed using the same placebo cream (PC) as base cream. One formulation contained balsam of oriental sweet gum, or Levant storax, named as Levant Storax Cream (LSC); the other contained oil of Calendula, extract of St. John’s Wort, aescin (an extract of horse chestnut), and freeze-dried powder from Aloe vera (L.) Burm. f. leaf juice, designated as Complex Cream (CC). In the characterization of the creams, organoleptic properties, pH, viscosity, size distribution, and zeta potential of oil globules were measured. Furthermore, the stability of the creams was assessed under different environmental conditions. In vitro studies were performed by using an excisional wound model in rats to assess the potential of the creams for stimulating wound healing. The efficacy of LSC and CC was compared with a commercial reference cream, Madecassol<sup>®</sup> (M), and the placebo control. The study was also designed with a negative control group of rats that were not treated but handled the same way as the other treatment groups. The wound contraction rate, total skin thickness recovery, and results of histopathological parameter examinations were used to compare the effectiveness of the treatments. <b>Results:</b> The stability of formulated creams confirmed that they were stable for the duration of the study. In vivo studies showed that rats treated with LSC achieved the highest wound healing rates when compared with the other groups. A better response was recorded for the CC-treated population when compared to both control and placebo groups, but there was no significant difference seen in healing score between CC and M groups.
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publishDate 2025-07-01
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spelling doaj-art-d8b020d26c844dc79e4f8da9926a54ac2025-08-20T03:32:18ZengMDPI AGPharmaceutics1999-49232025-07-0117794110.3390/pharmaceutics17070941Wound Healing Effects of New Cream Formulations with Herbal IngredientsDerya Algül0Ertuğrul Kılıç1Ferda Özkan2Yasemin Yağan Uzuner3Department of Pharmaceutical Technology, Faculty of Pharmacy, Yeditepe University, 34755 Kayışdağı, Istanbul, TurkeyDepartment of Physiology, School of Medicine, Medeniyet University, 34700 Uskudar, Istanbul, TurkeyDepartment of Breast Diseases and Breast Cancer, Istanbul Oncology Hospital, 34846 Maltepe, Istanbul, TurkeyDepartment of Pharmaceutical Technology, Faculty of Pharmacy, Yeditepe University, 34755 Kayışdağı, Istanbul, Turkey<b>Aim</b>: To prepare two different kinds of wound care creams containing plant extracts and examine their effectiveness in comparison with a placebo cream and a commercial wound care cream, Madecassol<sup>®</sup>. <b>Methods</b>: The two cream formulations were developed using the same placebo cream (PC) as base cream. One formulation contained balsam of oriental sweet gum, or Levant storax, named as Levant Storax Cream (LSC); the other contained oil of Calendula, extract of St. John’s Wort, aescin (an extract of horse chestnut), and freeze-dried powder from Aloe vera (L.) Burm. f. leaf juice, designated as Complex Cream (CC). In the characterization of the creams, organoleptic properties, pH, viscosity, size distribution, and zeta potential of oil globules were measured. Furthermore, the stability of the creams was assessed under different environmental conditions. In vitro studies were performed by using an excisional wound model in rats to assess the potential of the creams for stimulating wound healing. The efficacy of LSC and CC was compared with a commercial reference cream, Madecassol<sup>®</sup> (M), and the placebo control. The study was also designed with a negative control group of rats that were not treated but handled the same way as the other treatment groups. The wound contraction rate, total skin thickness recovery, and results of histopathological parameter examinations were used to compare the effectiveness of the treatments. <b>Results:</b> The stability of formulated creams confirmed that they were stable for the duration of the study. In vivo studies showed that rats treated with LSC achieved the highest wound healing rates when compared with the other groups. A better response was recorded for the CC-treated population when compared to both control and placebo groups, but there was no significant difference seen in healing score between CC and M groups.https://www.mdpi.com/1999-4923/17/7/941wound carestorax (<i>Liquidambar orientalis</i> Mill.) <i>Calendula officinalis</i>St. John’s Wortaescin
spellingShingle Derya Algül
Ertuğrul Kılıç
Ferda Özkan
Yasemin Yağan Uzuner
Wound Healing Effects of New Cream Formulations with Herbal Ingredients
Pharmaceutics
wound care
storax (<i>Liquidambar orientalis</i> Mill.) <i>Calendula officinalis</i>
St. John’s Wort
aescin
title Wound Healing Effects of New Cream Formulations with Herbal Ingredients
title_full Wound Healing Effects of New Cream Formulations with Herbal Ingredients
title_fullStr Wound Healing Effects of New Cream Formulations with Herbal Ingredients
title_full_unstemmed Wound Healing Effects of New Cream Formulations with Herbal Ingredients
title_short Wound Healing Effects of New Cream Formulations with Herbal Ingredients
title_sort wound healing effects of new cream formulations with herbal ingredients
topic wound care
storax (<i>Liquidambar orientalis</i> Mill.) <i>Calendula officinalis</i>
St. John’s Wort
aescin
url https://www.mdpi.com/1999-4923/17/7/941
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AT ertugrulkılıc woundhealingeffectsofnewcreamformulationswithherbalingredients
AT ferdaozkan woundhealingeffectsofnewcreamformulationswithherbalingredients
AT yaseminyaganuzuner woundhealingeffectsofnewcreamformulationswithherbalingredients