Simultaneous determination of ten nucleosides and bases in Ganoderma by micellar electrokinetic chromatography
Ganoderma (lingzhi) is a famous herbal medicine and edible supplement in oriental countries for a long history. In this study, a simple micellar electrokinetic chromatography (MEKC) method was established for the analysis of nucleosides and bases, the major bioactive components in Ganoderma for the...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2022-03-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453021001117 |
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| author | Feiya Sheng Songsong Wang Xiao Luo Jianbo Xiao Linfeng Hu Peng Li |
| author_facet | Feiya Sheng Songsong Wang Xiao Luo Jianbo Xiao Linfeng Hu Peng Li |
| author_sort | Feiya Sheng |
| collection | DOAJ |
| description | Ganoderma (lingzhi) is a famous herbal medicine and edible supplement in oriental countries for a long history. In this study, a simple micellar electrokinetic chromatography (MEKC) method was established for the analysis of nucleosides and bases, the major bioactive components in Ganoderma for the first time. By optimizing the borate concentration, the sodium dodecyl sulfate (SDS) concentration and the pH value of running buffer, 10 nucleosides and bases achieved an ideal separation. In real sample analysis, the developed method was successfully used to determine the 10 target analytes in 23 batches of Ganoderma samples from different regions. Results indicated that contents of 10 investigated analytes in each sample showed obvious variation. The principal components analysis (PCA) and hierarchical cluster analysis (HCA) analysis classified the samples into three groups, and the HCA tree visualized the relationships which was mainly contributed by geographical partition. The results indicated geographical origin to be an important factor that affect the accumulation of nucleosides and bases in Ganoderma. In summary, this study provides a simple and practical strategy for quality assessment and cultivation reference of Ganoderma. |
| format | Article |
| id | doaj-art-d88ae107bd444ff8a694e6b65c4ef8d7 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-d88ae107bd444ff8a694e6b65c4ef8d72025-02-03T10:31:08ZengTsinghua University PressFood Science and Human Wellness2213-45302022-03-01112263268Simultaneous determination of ten nucleosides and bases in Ganoderma by micellar electrokinetic chromatographyFeiya Sheng0Songsong Wang1Xiao Luo2Jianbo Xiao3Linfeng Hu4Peng Li5State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, 999078, China; School of Basic Medical Sciences, Chengdu University, Chengdu 610106, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, 999078, ChinaChengdu Institute for Food and Drug Control, Chengdu 610045, ChinaState Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, 999078, ChinaChongqing Key Laboratory of Medicinal Resources in the Three Gorges Reservoir Region, School of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China; Corresponding authors.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, 999078, China; Corresponding authors.Ganoderma (lingzhi) is a famous herbal medicine and edible supplement in oriental countries for a long history. In this study, a simple micellar electrokinetic chromatography (MEKC) method was established for the analysis of nucleosides and bases, the major bioactive components in Ganoderma for the first time. By optimizing the borate concentration, the sodium dodecyl sulfate (SDS) concentration and the pH value of running buffer, 10 nucleosides and bases achieved an ideal separation. In real sample analysis, the developed method was successfully used to determine the 10 target analytes in 23 batches of Ganoderma samples from different regions. Results indicated that contents of 10 investigated analytes in each sample showed obvious variation. The principal components analysis (PCA) and hierarchical cluster analysis (HCA) analysis classified the samples into three groups, and the HCA tree visualized the relationships which was mainly contributed by geographical partition. The results indicated geographical origin to be an important factor that affect the accumulation of nucleosides and bases in Ganoderma. In summary, this study provides a simple and practical strategy for quality assessment and cultivation reference of Ganoderma.http://www.sciencedirect.com/science/article/pii/S2213453021001117GanodermaNucleosidesBasesMicellar electrokinetic chromatographyQuality assessment |
| spellingShingle | Feiya Sheng Songsong Wang Xiao Luo Jianbo Xiao Linfeng Hu Peng Li Simultaneous determination of ten nucleosides and bases in Ganoderma by micellar electrokinetic chromatography Food Science and Human Wellness Ganoderma Nucleosides Bases Micellar electrokinetic chromatography Quality assessment |
| title | Simultaneous determination of ten nucleosides and bases in Ganoderma by micellar electrokinetic chromatography |
| title_full | Simultaneous determination of ten nucleosides and bases in Ganoderma by micellar electrokinetic chromatography |
| title_fullStr | Simultaneous determination of ten nucleosides and bases in Ganoderma by micellar electrokinetic chromatography |
| title_full_unstemmed | Simultaneous determination of ten nucleosides and bases in Ganoderma by micellar electrokinetic chromatography |
| title_short | Simultaneous determination of ten nucleosides and bases in Ganoderma by micellar electrokinetic chromatography |
| title_sort | simultaneous determination of ten nucleosides and bases in ganoderma by micellar electrokinetic chromatography |
| topic | Ganoderma Nucleosides Bases Micellar electrokinetic chromatography Quality assessment |
| url | http://www.sciencedirect.com/science/article/pii/S2213453021001117 |
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