Effects of the Rho-Kinase Inhibitor Y-27632 on Extraocular Muscle Surgery in Rabbits
Purpose. To evaluate the effect of the Rho-kinase inhibitor Y-27632 on postoperative inflammation and adhesion following extraocular muscle surgery in rabbits. Methods. The superior rectus muscle reinsertion was performed on both eyes of 8 New Zealand white rabbits. After reinsertion, the rabbits re...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2017/8653130 |
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| author | Ji‐Sun Moon Hyun Kyung Kim Sun Young Shin |
| author_facet | Ji‐Sun Moon Hyun Kyung Kim Sun Young Shin |
| author_sort | Ji‐Sun Moon |
| collection | DOAJ |
| description | Purpose. To evaluate the effect of the Rho-kinase inhibitor Y-27632 on postoperative inflammation and adhesion following extraocular muscle surgery in rabbits. Methods. The superior rectus muscle reinsertion was performed on both eyes of 8 New Zealand white rabbits. After reinsertion, the rabbits received subconjunctival injections of the Rho-kinase inhibitor and saline on each eye. To assess acute and late inflammatory changes, Ki-67, CD11β+, and F4/80 were evaluated and the sites of muscle reattachment were evaluated for a postoperative adhesion score and histopathologically for collagen formation. Results. F4/80 antibody expression was significantly different in the Rho-kinase inhibitor-injected group at both postoperative day 3 and week 4 (p=0.038, 0.031). However, Ki-67 and CD11β+ were not different the between two groups. The difference in the SRM/conjunctiva adhesion score between the two groups was also significant (p=0.034). Conclusion. Intraoperative subconjunctival injection of the Rho-kinase inhibitor may be effective for adjunctive management of inflammation and fibrosis in rabbit eyes following extraocular muscle surgery. |
| format | Article |
| id | doaj-art-d884a8a0bd6f4c81b45f2450d3b2c9e7 |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-d884a8a0bd6f4c81b45f2450d3b2c9e72025-02-03T01:32:55ZengWileyJournal of Ophthalmology2090-004X2090-00582017-01-01201710.1155/2017/86531308653130Effects of the Rho-Kinase Inhibitor Y-27632 on Extraocular Muscle Surgery in RabbitsJi‐Sun Moon0Hyun Kyung Kim1Sun Young Shin2Department of Ophthalmology and Visual Science, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, No. 222 Banpo-daero, Seocho-gu, Seoul 06591, Republic of KoreaDepartment of Ophthalmology, St. Vincent’s Hospital, College of Medicine, The Catholic University of Korea, No. 93 Jungbu-daero, Paldal-gu, Suwon 16247, Republic of KoreaDepartment of Ophthalmology and Visual Science, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, No. 222 Banpo-daero, Seocho-gu, Seoul 06591, Republic of KoreaPurpose. To evaluate the effect of the Rho-kinase inhibitor Y-27632 on postoperative inflammation and adhesion following extraocular muscle surgery in rabbits. Methods. The superior rectus muscle reinsertion was performed on both eyes of 8 New Zealand white rabbits. After reinsertion, the rabbits received subconjunctival injections of the Rho-kinase inhibitor and saline on each eye. To assess acute and late inflammatory changes, Ki-67, CD11β+, and F4/80 were evaluated and the sites of muscle reattachment were evaluated for a postoperative adhesion score and histopathologically for collagen formation. Results. F4/80 antibody expression was significantly different in the Rho-kinase inhibitor-injected group at both postoperative day 3 and week 4 (p=0.038, 0.031). However, Ki-67 and CD11β+ were not different the between two groups. The difference in the SRM/conjunctiva adhesion score between the two groups was also significant (p=0.034). Conclusion. Intraoperative subconjunctival injection of the Rho-kinase inhibitor may be effective for adjunctive management of inflammation and fibrosis in rabbit eyes following extraocular muscle surgery.http://dx.doi.org/10.1155/2017/8653130 |
| spellingShingle | Ji‐Sun Moon Hyun Kyung Kim Sun Young Shin Effects of the Rho-Kinase Inhibitor Y-27632 on Extraocular Muscle Surgery in Rabbits Journal of Ophthalmology |
| title | Effects of the Rho-Kinase Inhibitor Y-27632 on Extraocular Muscle Surgery in Rabbits |
| title_full | Effects of the Rho-Kinase Inhibitor Y-27632 on Extraocular Muscle Surgery in Rabbits |
| title_fullStr | Effects of the Rho-Kinase Inhibitor Y-27632 on Extraocular Muscle Surgery in Rabbits |
| title_full_unstemmed | Effects of the Rho-Kinase Inhibitor Y-27632 on Extraocular Muscle Surgery in Rabbits |
| title_short | Effects of the Rho-Kinase Inhibitor Y-27632 on Extraocular Muscle Surgery in Rabbits |
| title_sort | effects of the rho kinase inhibitor y 27632 on extraocular muscle surgery in rabbits |
| url | http://dx.doi.org/10.1155/2017/8653130 |
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