L-theanine modulates tissue cortisol levels in zebrafish after single stress
Abstract L-theanine has neuromodulatory properties, such as attenuate anxiety disorders and stress. Stress is a situation characterized by the stimulus to cortisol secretion and without specific pharmacological therapies. Thus, this study investigates the effect of L-theanine on the response to acut...
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2025-01-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502025000100301&lng=en&tlng=en |
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| author | Vinícius Luís da Silva Mariana Spanamberg Mayer Andriele dos Reis Severo Andressa Leal Zambra Joanna Moraes De Almeida Laura Moura Sestari Mariana Migliorini Parisi Ana Cristina Vendrametto Varrone Giacomini Murilo Sander De Abreu Josiane Woutheres Bortolotto Gabriela Bonfanti-Azzolin |
| author_facet | Vinícius Luís da Silva Mariana Spanamberg Mayer Andriele dos Reis Severo Andressa Leal Zambra Joanna Moraes De Almeida Laura Moura Sestari Mariana Migliorini Parisi Ana Cristina Vendrametto Varrone Giacomini Murilo Sander De Abreu Josiane Woutheres Bortolotto Gabriela Bonfanti-Azzolin |
| author_sort | Vinícius Luís da Silva |
| collection | DOAJ |
| description | Abstract L-theanine has neuromodulatory properties, such as attenuate anxiety disorders and stress. Stress is a situation characterized by the stimulus to cortisol secretion and without specific pharmacological therapies. Thus, this study investigates the effect of L-theanine on the response to acute stress, evaluated by tissue cortisol level, oxidative status markers, and behavioral parameters, in zebrafish. The animals were divided into six groups: G1: control, G2: treated with L-theanine 45 mg/L, G3: treated with L-theanine 100 mg/L, G4: stress, G5: stress+L-theanine 45 mg/L and G6: stress+L-theanine 100 mg/L. Before the execution of the acute stress protocol that consisted of chasing the animals with a net for two minutes, exposure to L-theanine was performed in aquarium water for 1 hour. Tissue cortisol levels were analyzed, as well as cerebral oxidative status (lipoperoxidation and levels of thiolic compounds) and fish behavior (new tank test). L-theanine at a concentration of 45 mg/mL modulated the effects of acute stress on zebrafish, inhibiting increased levels of tissue cortisol. However, it was not able to alter the behavior or oxidative status of the animals. Thus, we conclude that the administration of L-theanine can promote acute stress attenuation, modulating the pathological responses of stress. |
| format | Article |
| id | doaj-art-d869f2fb44e34d1cb12677e1aa4be63b |
| institution | Kabale University |
| issn | 2175-9790 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj-art-d869f2fb44e34d1cb12677e1aa4be63b2025-01-21T07:41:47ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902025-01-016110.1590/s2175-97902025e23375L-theanine modulates tissue cortisol levels in zebrafish after single stressVinícius Luís da Silvahttps://orcid.org/0000-0002-8887-7872Mariana Spanamberg Mayerhttps://orcid.org/0000-0002-2514-3783Andriele dos Reis Severohttps://orcid.org/0000-0001-9987-0521Andressa Leal Zambrahttps://orcid.org/0000-0002-1607-2741Joanna Moraes De Almeidahttps://orcid.org/0000-0001-8001-3124Laura Moura Sestarihttps://orcid.org/0000-0001-8194-1563Mariana Migliorini Parisihttps://orcid.org/0000-0002-2298-7809Ana Cristina Vendrametto Varrone Giacominihttps://orcid.org/0000-0003-2102-512XMurilo Sander De Abreuhttps://orcid.org/0000-0001-5562-0715Josiane Woutheres Bortolottohttps://orcid.org/0000-0003-0271-1685Gabriela Bonfanti-Azzolinhttps://orcid.org/0000-0003-2602-6092Abstract L-theanine has neuromodulatory properties, such as attenuate anxiety disorders and stress. Stress is a situation characterized by the stimulus to cortisol secretion and without specific pharmacological therapies. Thus, this study investigates the effect of L-theanine on the response to acute stress, evaluated by tissue cortisol level, oxidative status markers, and behavioral parameters, in zebrafish. The animals were divided into six groups: G1: control, G2: treated with L-theanine 45 mg/L, G3: treated with L-theanine 100 mg/L, G4: stress, G5: stress+L-theanine 45 mg/L and G6: stress+L-theanine 100 mg/L. Before the execution of the acute stress protocol that consisted of chasing the animals with a net for two minutes, exposure to L-theanine was performed in aquarium water for 1 hour. Tissue cortisol levels were analyzed, as well as cerebral oxidative status (lipoperoxidation and levels of thiolic compounds) and fish behavior (new tank test). L-theanine at a concentration of 45 mg/mL modulated the effects of acute stress on zebrafish, inhibiting increased levels of tissue cortisol. However, it was not able to alter the behavior or oxidative status of the animals. Thus, we conclude that the administration of L-theanine can promote acute stress attenuation, modulating the pathological responses of stress.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502025000100301&lng=en&tlng=enStressL-theanineZebrafish |
| spellingShingle | Vinícius Luís da Silva Mariana Spanamberg Mayer Andriele dos Reis Severo Andressa Leal Zambra Joanna Moraes De Almeida Laura Moura Sestari Mariana Migliorini Parisi Ana Cristina Vendrametto Varrone Giacomini Murilo Sander De Abreu Josiane Woutheres Bortolotto Gabriela Bonfanti-Azzolin L-theanine modulates tissue cortisol levels in zebrafish after single stress Brazilian Journal of Pharmaceutical Sciences Stress L-theanine Zebrafish |
| title | L-theanine modulates tissue cortisol levels in zebrafish after single stress |
| title_full | L-theanine modulates tissue cortisol levels in zebrafish after single stress |
| title_fullStr | L-theanine modulates tissue cortisol levels in zebrafish after single stress |
| title_full_unstemmed | L-theanine modulates tissue cortisol levels in zebrafish after single stress |
| title_short | L-theanine modulates tissue cortisol levels in zebrafish after single stress |
| title_sort | l theanine modulates tissue cortisol levels in zebrafish after single stress |
| topic | Stress L-theanine Zebrafish |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502025000100301&lng=en&tlng=en |
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