Integrating transcriptomics, metabolomics, and network pharmacology to investigate multi-target effects of sporoderm-broken spores of Ganoderma lucidum on improving HFD-induced diabetic nephropathy rats
Diabetes mellitus (DM) is a major metabolic disease endangering global health, with diabetic nephropathy (DN) as a primary complication lacking curative therapy. Sporoderm-broken spores of Ganoderma lucidum (GLP), an herbal medicine, has been used for the treatment of metabolic disorders. In this st...
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Elsevier
2024-12-01
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| Series: | Journal of Pharmaceutical Analysis |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095177924002028 |
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| author | Lidan Hu Lili Yu Zhongkai Cao Yue Wang Caifeng Zhu Yayu Li Jiazhen Yin Zhichao Ma Xuelin He Ying Zhang Wunan Huang Yuelin Guan Yue Chen Xue Li Xiangjun Chen |
| author_facet | Lidan Hu Lili Yu Zhongkai Cao Yue Wang Caifeng Zhu Yayu Li Jiazhen Yin Zhichao Ma Xuelin He Ying Zhang Wunan Huang Yuelin Guan Yue Chen Xue Li Xiangjun Chen |
| author_sort | Lidan Hu |
| collection | DOAJ |
| description | Diabetes mellitus (DM) is a major metabolic disease endangering global health, with diabetic nephropathy (DN) as a primary complication lacking curative therapy. Sporoderm-broken spores of Ganoderma lucidum (GLP), an herbal medicine, has been used for the treatment of metabolic disorders. In this study, DN was induced in Sprague-Dawley rats using streptozotocin (STZ) and a high-fat diet (HFD), and the protective mechanisms of GLP were investigated through transcriptomic, metabolomic, and network pharmacology (NP) analyses. Our results demonstrated that GLP intervention ameliorated renal damage and inflammation levels in DN rats. Integrative metabolomic and transcriptomic analysis revealed that GLP treatment modulated glucose and cellular energy metabolisms by regulating relevant genes. GLP significantly suppressed the inflammations by impacting glucose and energy metabolism-related gene expression (Igfbp1 and Angptl4) and enhanced metabolic biomarkers of 4-Aminocatechol. In addition, NP analysis further indicated that GLP may efficiently alleviate DN via immune-related pathways. In conclusion, this study provides supportive evidence of the anti-inflammatory effects of GLP supplements, highlighting their potential for promising clinical applications in treating DN. |
| format | Article |
| id | doaj-art-d869a9bfa7654de2b01d86d509526bcd |
| institution | Kabale University |
| issn | 2095-1779 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmaceutical Analysis |
| spelling | doaj-art-d869a9bfa7654de2b01d86d509526bcd2025-01-30T05:13:59ZengElsevierJournal of Pharmaceutical Analysis2095-17792024-12-011412101105Integrating transcriptomics, metabolomics, and network pharmacology to investigate multi-target effects of sporoderm-broken spores of Ganoderma lucidum on improving HFD-induced diabetic nephropathy ratsLidan Hu0Lili Yu1Zhongkai Cao2Yue Wang3Caifeng Zhu4Yayu Li5Jiazhen Yin6Zhichao Ma7Xuelin He8Ying Zhang9Wunan Huang10Yuelin Guan11Yue Chen12Xue Li13Xiangjun Chen14Department of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310003, ChinaCentre for Global Health, Usher Institute, The University of Edinburgh, Edinburgh, EH16 4UX, UK; Department of Big Data in Health Science School of Public Health, Zhejiang University School of Medicine, Hangzhou, 310058, ChinaDepartment of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310003, ChinaHubei Normal University, Huangshi, Hubei, 435002, ChinaDepartment of Nephrology, Hangzhou TCM Hospital, Hangzhou, 310007, ChinaDepartment of Nephrology, Hangzhou TCM Hospital, Hangzhou, 310007, ChinaDepartment of Nephrology, Hangzhou TCM Hospital, Hangzhou, 310007, ChinaDepartment of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310003, ChinaKidney Disease Center, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, ChinaInstitute of Translational Medicine, Zhejiang University School of Medicine, Hangzhou, 310020, ChinaDepartment of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310003, ChinaDepartment of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310003, ChinaDepartment of Urology, The Second Hospital of Tianjin Medical University, Tianjin Institute of Urology, Tianjin, 300211, China; Corresponding author.Department of Big Data in Health Science School of Public Health, Zhejiang University School of Medicine, Hangzhou, 310058, China; Corresponding author.Institute of Translational Medicine, Zhejiang University School of Medicine, Hangzhou, 310020, China; Corresponding author.Diabetes mellitus (DM) is a major metabolic disease endangering global health, with diabetic nephropathy (DN) as a primary complication lacking curative therapy. Sporoderm-broken spores of Ganoderma lucidum (GLP), an herbal medicine, has been used for the treatment of metabolic disorders. In this study, DN was induced in Sprague-Dawley rats using streptozotocin (STZ) and a high-fat diet (HFD), and the protective mechanisms of GLP were investigated through transcriptomic, metabolomic, and network pharmacology (NP) analyses. Our results demonstrated that GLP intervention ameliorated renal damage and inflammation levels in DN rats. Integrative metabolomic and transcriptomic analysis revealed that GLP treatment modulated glucose and cellular energy metabolisms by regulating relevant genes. GLP significantly suppressed the inflammations by impacting glucose and energy metabolism-related gene expression (Igfbp1 and Angptl4) and enhanced metabolic biomarkers of 4-Aminocatechol. In addition, NP analysis further indicated that GLP may efficiently alleviate DN via immune-related pathways. In conclusion, this study provides supportive evidence of the anti-inflammatory effects of GLP supplements, highlighting their potential for promising clinical applications in treating DN.http://www.sciencedirect.com/science/article/pii/S2095177924002028Diabetic nephropathyMetabolomeNetwork pharmacologySporoderm-broken spores of Ganoderma lucidumTranscriptome |
| spellingShingle | Lidan Hu Lili Yu Zhongkai Cao Yue Wang Caifeng Zhu Yayu Li Jiazhen Yin Zhichao Ma Xuelin He Ying Zhang Wunan Huang Yuelin Guan Yue Chen Xue Li Xiangjun Chen Integrating transcriptomics, metabolomics, and network pharmacology to investigate multi-target effects of sporoderm-broken spores of Ganoderma lucidum on improving HFD-induced diabetic nephropathy rats Journal of Pharmaceutical Analysis Diabetic nephropathy Metabolome Network pharmacology Sporoderm-broken spores of Ganoderma lucidum Transcriptome |
| title | Integrating transcriptomics, metabolomics, and network pharmacology to investigate multi-target effects of sporoderm-broken spores of Ganoderma lucidum on improving HFD-induced diabetic nephropathy rats |
| title_full | Integrating transcriptomics, metabolomics, and network pharmacology to investigate multi-target effects of sporoderm-broken spores of Ganoderma lucidum on improving HFD-induced diabetic nephropathy rats |
| title_fullStr | Integrating transcriptomics, metabolomics, and network pharmacology to investigate multi-target effects of sporoderm-broken spores of Ganoderma lucidum on improving HFD-induced diabetic nephropathy rats |
| title_full_unstemmed | Integrating transcriptomics, metabolomics, and network pharmacology to investigate multi-target effects of sporoderm-broken spores of Ganoderma lucidum on improving HFD-induced diabetic nephropathy rats |
| title_short | Integrating transcriptomics, metabolomics, and network pharmacology to investigate multi-target effects of sporoderm-broken spores of Ganoderma lucidum on improving HFD-induced diabetic nephropathy rats |
| title_sort | integrating transcriptomics metabolomics and network pharmacology to investigate multi target effects of sporoderm broken spores of ganoderma lucidum on improving hfd induced diabetic nephropathy rats |
| topic | Diabetic nephropathy Metabolome Network pharmacology Sporoderm-broken spores of Ganoderma lucidum Transcriptome |
| url | http://www.sciencedirect.com/science/article/pii/S2095177924002028 |
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