Blueberry malvidin-3-galactoside modulated gut microbial dysbiosis and microbial TCA cycle KEGG pathway disrupted in a liver cancer model induced by HepG2 cells
HCC (Hepatocellular Carcinoma) is a critical health issue worldwide. Our previous animal experiment has confirmed that blueberry malvidin-3-galactoside (M3G) can regulate the progression of HCC. In this study, feces samples from the same batch of mice were collected to explore the regulatory mechani...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2020-09-01
|
| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453020301348 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832557338518618112 |
|---|---|
| author | Zhen Cheng Jie Lin Ningxuan Gao Xiyun Sun Xianjun Meng Ruihai Liu Yunen Liu Weisheng Wang Bin Li Yuehua Wang |
| author_facet | Zhen Cheng Jie Lin Ningxuan Gao Xiyun Sun Xianjun Meng Ruihai Liu Yunen Liu Weisheng Wang Bin Li Yuehua Wang |
| author_sort | Zhen Cheng |
| collection | DOAJ |
| description | HCC (Hepatocellular Carcinoma) is a critical health issue worldwide. Our previous animal experiment has confirmed that blueberry malvidin-3-galactoside (M3G) can regulate the progression of HCC. In this study, feces samples from the same batch of mice were collected to explore the regulatory mechanism of M3G on intestinal microbiota and microbial TCA cycle metabolism KEGG pathway in HCC mice based on 16S rRNA sequencing and metagenomics. Our results showed that blueberry M3G increased the microbial diversity and regulated the structure of intestinal microbiota in mice, such as increasing the abundance of Clostridia (butyric acid-producing bacteria), Oscillospira and Ruminococcus, and reducing the abundance of pathogenic Erysipelotrichi. Compared with the group of liver cancer (LC) and 5-fluorouracil (5 F), blueberry M3G significantly regulated microbial TCA cycle KEGG pathway via improving the expression of key proteins (porA, DLAT, aceE, PC and OGDH). Additionally, we found which the abundance of Muribaculum intestinale (M.intestinale) increased by blueberry M3G may be an important factor affecting the microbial TCA cycle KEGG pathway via the pearson correlation (R) analysis of protein and microbiota. Taken together, these results demonstrate that the blueberry M3G has the potential to be an intestinal microbiota regulator and an adjuvant to HCC therapy. |
| format | Article |
| id | doaj-art-d85403dd446c4ced8f121d21db1c06d8 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2020-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-d85403dd446c4ced8f121d21db1c06d82025-02-03T05:21:06ZengTsinghua University PressFood Science and Human Wellness2213-45302020-09-0193245255Blueberry malvidin-3-galactoside modulated gut microbial dysbiosis and microbial TCA cycle KEGG pathway disrupted in a liver cancer model induced by HepG2 cellsZhen Cheng0Jie Lin1Ningxuan Gao2Xiyun Sun3Xianjun Meng4Ruihai Liu5Yunen Liu6Weisheng Wang7Bin Li8Yuehua Wang9College of Food Science, Shenyang Agricultural University, Liaoning, PR China; Key Laboratory of Healthy Food Nutrition and Innovative Manufacturing of Liaoning, PR ChinaCollege of Food Science, Shenyang Agricultural University, Liaoning, PR China; Key Laboratory of Healthy Food Nutrition and Innovative Manufacturing of Liaoning, PR ChinaCollege of Food Science, Shenyang Agricultural University, Liaoning, PR China; Key Laboratory of Healthy Food Nutrition and Innovative Manufacturing of Liaoning, PR ChinaCollege of Food Science, Shenyang Agricultural University, Liaoning, PR China; National R&D Professional Center For Berry Processing, Shenyang Agricultural University, Liaoning, PR China; Key Laboratory of Healthy Food Nutrition and Innovative Manufacturing of Liaoning, PR ChinaCollege of Food Science, Shenyang Agricultural University, Liaoning, PR China; National R&D Professional Center For Berry Processing, Shenyang Agricultural University, Liaoning, PR ChinaCollege of Food Science, Shenyang Agricultural University, Liaoning, PR China; National R&D Professional Center For Berry Processing, Shenyang Agricultural University, Liaoning, PR China; Department of food Science, Cornell University, Ithaca, NY, USAEmergency Medicine Department of General Hospital of Shenyang Military Command, Laboratory of Rescue Center of Severe Trauma PLA, Liaoning, PR ChinaShenyang Blueberry Industry Co. LTD., Liaoning, PR ChinaCollege of Food Science, Shenyang Agricultural University, Liaoning, PR China; National R&D Professional Center For Berry Processing, Shenyang Agricultural University, Liaoning, PR China; Key Laboratory of Healthy Food Nutrition and Innovative Manufacturing of Liaoning, PR China; Corresponding authors at: Dong ling Road, Shen he District, Shenyang, Liaoning, 110866, PR China.College of Food Science, Shenyang Agricultural University, Liaoning, PR China; National R&D Professional Center For Berry Processing, Shenyang Agricultural University, Liaoning, PR China; Key Laboratory of Healthy Food Nutrition and Innovative Manufacturing of Liaoning, PR China; Corresponding authors at: Dong ling Road, Shen he District, Shenyang, Liaoning, 110866, PR China.HCC (Hepatocellular Carcinoma) is a critical health issue worldwide. Our previous animal experiment has confirmed that blueberry malvidin-3-galactoside (M3G) can regulate the progression of HCC. In this study, feces samples from the same batch of mice were collected to explore the regulatory mechanism of M3G on intestinal microbiota and microbial TCA cycle metabolism KEGG pathway in HCC mice based on 16S rRNA sequencing and metagenomics. Our results showed that blueberry M3G increased the microbial diversity and regulated the structure of intestinal microbiota in mice, such as increasing the abundance of Clostridia (butyric acid-producing bacteria), Oscillospira and Ruminococcus, and reducing the abundance of pathogenic Erysipelotrichi. Compared with the group of liver cancer (LC) and 5-fluorouracil (5 F), blueberry M3G significantly regulated microbial TCA cycle KEGG pathway via improving the expression of key proteins (porA, DLAT, aceE, PC and OGDH). Additionally, we found which the abundance of Muribaculum intestinale (M.intestinale) increased by blueberry M3G may be an important factor affecting the microbial TCA cycle KEGG pathway via the pearson correlation (R) analysis of protein and microbiota. Taken together, these results demonstrate that the blueberry M3G has the potential to be an intestinal microbiota regulator and an adjuvant to HCC therapy.http://www.sciencedirect.com/science/article/pii/S2213453020301348Blueberrymalvidin-3-galactosideHCCmetagenomicsmicrobial TCA cycle KEGG pathway |
| spellingShingle | Zhen Cheng Jie Lin Ningxuan Gao Xiyun Sun Xianjun Meng Ruihai Liu Yunen Liu Weisheng Wang Bin Li Yuehua Wang Blueberry malvidin-3-galactoside modulated gut microbial dysbiosis and microbial TCA cycle KEGG pathway disrupted in a liver cancer model induced by HepG2 cells Food Science and Human Wellness Blueberry malvidin-3-galactoside HCC metagenomics microbial TCA cycle KEGG pathway |
| title | Blueberry malvidin-3-galactoside modulated gut microbial dysbiosis and microbial TCA cycle KEGG pathway disrupted in a liver cancer model induced by HepG2 cells |
| title_full | Blueberry malvidin-3-galactoside modulated gut microbial dysbiosis and microbial TCA cycle KEGG pathway disrupted in a liver cancer model induced by HepG2 cells |
| title_fullStr | Blueberry malvidin-3-galactoside modulated gut microbial dysbiosis and microbial TCA cycle KEGG pathway disrupted in a liver cancer model induced by HepG2 cells |
| title_full_unstemmed | Blueberry malvidin-3-galactoside modulated gut microbial dysbiosis and microbial TCA cycle KEGG pathway disrupted in a liver cancer model induced by HepG2 cells |
| title_short | Blueberry malvidin-3-galactoside modulated gut microbial dysbiosis and microbial TCA cycle KEGG pathway disrupted in a liver cancer model induced by HepG2 cells |
| title_sort | blueberry malvidin 3 galactoside modulated gut microbial dysbiosis and microbial tca cycle kegg pathway disrupted in a liver cancer model induced by hepg2 cells |
| topic | Blueberry malvidin-3-galactoside HCC metagenomics microbial TCA cycle KEGG pathway |
| url | http://www.sciencedirect.com/science/article/pii/S2213453020301348 |
| work_keys_str_mv | AT zhencheng blueberrymalvidin3galactosidemodulatedgutmicrobialdysbiosisandmicrobialtcacyclekeggpathwaydisruptedinalivercancermodelinducedbyhepg2cells AT jielin blueberrymalvidin3galactosidemodulatedgutmicrobialdysbiosisandmicrobialtcacyclekeggpathwaydisruptedinalivercancermodelinducedbyhepg2cells AT ningxuangao blueberrymalvidin3galactosidemodulatedgutmicrobialdysbiosisandmicrobialtcacyclekeggpathwaydisruptedinalivercancermodelinducedbyhepg2cells AT xiyunsun blueberrymalvidin3galactosidemodulatedgutmicrobialdysbiosisandmicrobialtcacyclekeggpathwaydisruptedinalivercancermodelinducedbyhepg2cells AT xianjunmeng blueberrymalvidin3galactosidemodulatedgutmicrobialdysbiosisandmicrobialtcacyclekeggpathwaydisruptedinalivercancermodelinducedbyhepg2cells AT ruihailiu blueberrymalvidin3galactosidemodulatedgutmicrobialdysbiosisandmicrobialtcacyclekeggpathwaydisruptedinalivercancermodelinducedbyhepg2cells AT yunenliu blueberrymalvidin3galactosidemodulatedgutmicrobialdysbiosisandmicrobialtcacyclekeggpathwaydisruptedinalivercancermodelinducedbyhepg2cells AT weishengwang blueberrymalvidin3galactosidemodulatedgutmicrobialdysbiosisandmicrobialtcacyclekeggpathwaydisruptedinalivercancermodelinducedbyhepg2cells AT binli blueberrymalvidin3galactosidemodulatedgutmicrobialdysbiosisandmicrobialtcacyclekeggpathwaydisruptedinalivercancermodelinducedbyhepg2cells AT yuehuawang blueberrymalvidin3galactosidemodulatedgutmicrobialdysbiosisandmicrobialtcacyclekeggpathwaydisruptedinalivercancermodelinducedbyhepg2cells |