Evaluation of the Sterile Insect Technique for the Control of the Melon Fly (<i>Bactrocera cucurbitae</i>) Under Laboratory and Semi-Field Conditions in Sri Lanka
The melon fly, <i>Bactrocera cucurbitae</i>, poses a severe threat to the country’s agricultural productivity, particularly in the cultivation of cucurbitaceous crops. This study was conducted to determine the ideal irradiation dose to be used to set up a Sterile Insect Technique (SIT)-b...
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2024-12-01
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| author | Muditha Nawarathne Lahiru Udayanga Hasini Ekanayake Bader Alhafi Alotaib Ananda Pathirage Nayana Siriwardena Athula Jayarathne Hassan Ammouneh Mohamed M. M. Najim Abou Traore Tharaka Ranathunge |
| author_facet | Muditha Nawarathne Lahiru Udayanga Hasini Ekanayake Bader Alhafi Alotaib Ananda Pathirage Nayana Siriwardena Athula Jayarathne Hassan Ammouneh Mohamed M. M. Najim Abou Traore Tharaka Ranathunge |
| author_sort | Muditha Nawarathne |
| collection | DOAJ |
| description | The melon fly, <i>Bactrocera cucurbitae</i>, poses a severe threat to the country’s agricultural productivity, particularly in the cultivation of cucurbitaceous crops. This study was conducted to determine the ideal irradiation dose to be used to set up a Sterile Insect Technique (SIT)-based strategy to control <i>B. cucurbitae</i> outbreaks in Sri Lanka. A <i>B. cucurbitae</i> colony was established and maintained under standard laboratory conditions. Male pupae (100 pupae per treatment) were exposed to different radiation doses, namely 0 (control), 50, 60, 70, 80, 90, 100, and 110 Gy. The effect of irradiation on selected performance parameters such as survival, flight ability, fecundity, fertility, and adult longevity was evaluated under laboratory conditions. In addition, mating competitiveness was evaluated for irradiated male <i>B. cucurbitae</i>. Significant differences were observed in percentage pupal survival, flight ability, and fertility rates (<i>p</i> < 0.05) at a 95% level of confidence. Further, the 50% and 75% adult longevity periods of irradiated <i>B. cucurbitae</i> males significantly decreased with the increasing irradiation dosage (<i>p</i> < 0.05). <i>B. cucurbitae</i> exposed to 70 Gy radiation reported a mean pupal survival rate of 94.0 ± 1.25, flight ability of 93.3 ± 1.88, and fertility 0.2 ± 0.05%, along with a mean longevity of 38 ± 1.5 days until 50% reduction in the population. The competitiveness of <i>B. cucurbitae</i> exposed to 70 Gy with respect to wild males was 0.56 under laboratory conditions, which reduced up to 0.5 in semi-field settings. The 70 Gy dose could be recommended as the optimal radiation dose for the production of sterile <i>B. cucurbitae</i> males with an acceptable level of fitness and sterility to be used in SIT-based melon fly control programmes in Sri Lanka. |
| format | Article |
| id | doaj-art-d843d74510d449c0b941008c693fce1e |
| institution | Kabale University |
| issn | 2075-4450 |
| language | English |
| publishDate | 2024-12-01 |
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| series | Insects |
| spelling | doaj-art-d843d74510d449c0b941008c693fce1e2025-01-24T13:35:36ZengMDPI AGInsects2075-44502024-12-011612110.3390/insects16010021Evaluation of the Sterile Insect Technique for the Control of the Melon Fly (<i>Bactrocera cucurbitae</i>) Under Laboratory and Semi-Field Conditions in Sri LankaMuditha Nawarathne0Lahiru Udayanga1Hasini Ekanayake2Bader Alhafi Alotaib3Ananda Pathirage4Nayana Siriwardena5Athula Jayarathne6Hassan Ammouneh7Mohamed M. M. Najim8Abou Traore9Tharaka Ranathunge10Hayleys Sunfrost (Pvt) Limited, Biyagama Export Processing Zone, Biyagama 11650, Sri LankaDepartment of Biosystems Engineering, Faculty of Agriculture and Plantation Management, Wayamba University of Sri Lanka, Makandura 81070, Sri LankaDepartment of Biosystems Engineering, Faculty of Agriculture and Plantation Management, Wayamba University of Sri Lanka, Makandura 81070, Sri LankaDepartment of Agricultural Extension and Rural Society, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Saudi ArabiaHayleys Sunfrost (Pvt) Limited, Biyagama Export Processing Zone, Biyagama 11650, Sri LankaHayleys Sunfrost (Pvt) Limited, Biyagama Export Processing Zone, Biyagama 11650, Sri LankaHayleys Sunfrost (Pvt) Limited, Biyagama Export Processing Zone, Biyagama 11650, Sri LankaFaculty of Agriculture, Sultan Sharif Ali Islamic University (UNISSA), Kampus Sinaut, Km 33, Jln Tutong, Kampong Sinaut, Tutong TB1741, BruneiFaculty of Agriculture, Sultan Sharif Ali Islamic University (UNISSA), Kampus Sinaut, Km 33, Jln Tutong, Kampong Sinaut, Tutong TB1741, BruneiDepartment of Community Sustainability, College of Agriculture and Natural Resources, Michigan State University, 328 Natural Resources Building, East Lansing, MI 48824, USADepartment of Zoology, Faculty of Science, Eastern University, Chenkalady 30350, Sri LankaThe melon fly, <i>Bactrocera cucurbitae</i>, poses a severe threat to the country’s agricultural productivity, particularly in the cultivation of cucurbitaceous crops. This study was conducted to determine the ideal irradiation dose to be used to set up a Sterile Insect Technique (SIT)-based strategy to control <i>B. cucurbitae</i> outbreaks in Sri Lanka. A <i>B. cucurbitae</i> colony was established and maintained under standard laboratory conditions. Male pupae (100 pupae per treatment) were exposed to different radiation doses, namely 0 (control), 50, 60, 70, 80, 90, 100, and 110 Gy. The effect of irradiation on selected performance parameters such as survival, flight ability, fecundity, fertility, and adult longevity was evaluated under laboratory conditions. In addition, mating competitiveness was evaluated for irradiated male <i>B. cucurbitae</i>. Significant differences were observed in percentage pupal survival, flight ability, and fertility rates (<i>p</i> < 0.05) at a 95% level of confidence. Further, the 50% and 75% adult longevity periods of irradiated <i>B. cucurbitae</i> males significantly decreased with the increasing irradiation dosage (<i>p</i> < 0.05). <i>B. cucurbitae</i> exposed to 70 Gy radiation reported a mean pupal survival rate of 94.0 ± 1.25, flight ability of 93.3 ± 1.88, and fertility 0.2 ± 0.05%, along with a mean longevity of 38 ± 1.5 days until 50% reduction in the population. The competitiveness of <i>B. cucurbitae</i> exposed to 70 Gy with respect to wild males was 0.56 under laboratory conditions, which reduced up to 0.5 in semi-field settings. The 70 Gy dose could be recommended as the optimal radiation dose for the production of sterile <i>B. cucurbitae</i> males with an acceptable level of fitness and sterility to be used in SIT-based melon fly control programmes in Sri Lanka.https://www.mdpi.com/2075-4450/16/1/21<i>Bactrocera cucurbitae</i>controlmelon flySterile Insect Technique (SIT)Sri Lanka |
| spellingShingle | Muditha Nawarathne Lahiru Udayanga Hasini Ekanayake Bader Alhafi Alotaib Ananda Pathirage Nayana Siriwardena Athula Jayarathne Hassan Ammouneh Mohamed M. M. Najim Abou Traore Tharaka Ranathunge Evaluation of the Sterile Insect Technique for the Control of the Melon Fly (<i>Bactrocera cucurbitae</i>) Under Laboratory and Semi-Field Conditions in Sri Lanka Insects <i>Bactrocera cucurbitae</i> control melon fly Sterile Insect Technique (SIT) Sri Lanka |
| title | Evaluation of the Sterile Insect Technique for the Control of the Melon Fly (<i>Bactrocera cucurbitae</i>) Under Laboratory and Semi-Field Conditions in Sri Lanka |
| title_full | Evaluation of the Sterile Insect Technique for the Control of the Melon Fly (<i>Bactrocera cucurbitae</i>) Under Laboratory and Semi-Field Conditions in Sri Lanka |
| title_fullStr | Evaluation of the Sterile Insect Technique for the Control of the Melon Fly (<i>Bactrocera cucurbitae</i>) Under Laboratory and Semi-Field Conditions in Sri Lanka |
| title_full_unstemmed | Evaluation of the Sterile Insect Technique for the Control of the Melon Fly (<i>Bactrocera cucurbitae</i>) Under Laboratory and Semi-Field Conditions in Sri Lanka |
| title_short | Evaluation of the Sterile Insect Technique for the Control of the Melon Fly (<i>Bactrocera cucurbitae</i>) Under Laboratory and Semi-Field Conditions in Sri Lanka |
| title_sort | evaluation of the sterile insect technique for the control of the melon fly i bactrocera cucurbitae i under laboratory and semi field conditions in sri lanka |
| topic | <i>Bactrocera cucurbitae</i> control melon fly Sterile Insect Technique (SIT) Sri Lanka |
| url | https://www.mdpi.com/2075-4450/16/1/21 |
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