Clinical Specimens are the Pool of Multidrug- resistant Pseudomonas aeruginosa Harbouring oprL and toxA Virulence Genes: Findings from a Tertiary Hospital of Nepal
The multidrug- or extensively drug-resistant (MDR/XDR) Pseudomonas aeruginosa carrying some virulence genes has become a global public health threat. However, in Nepal, there is no existing report showing the prevalence of oprL and toxA virulence genes among the clinical isolates of P. aeruginosa. T...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | Emergency Medicine International |
| Online Access: | http://dx.doi.org/10.1155/2021/4120697 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832568528952098816 |
|---|---|
| author | Yamuna Chand Sujan Khadka Sanjeep Sapkota Suprina Sharma Santosh Khanal Alina Thapa Binod Rayamajhee Dhruba Kumar Khadka Om Prakash Panta Dipendra Shrestha Pramod Poudel |
| author_facet | Yamuna Chand Sujan Khadka Sanjeep Sapkota Suprina Sharma Santosh Khanal Alina Thapa Binod Rayamajhee Dhruba Kumar Khadka Om Prakash Panta Dipendra Shrestha Pramod Poudel |
| author_sort | Yamuna Chand |
| collection | DOAJ |
| description | The multidrug- or extensively drug-resistant (MDR/XDR) Pseudomonas aeruginosa carrying some virulence genes has become a global public health threat. However, in Nepal, there is no existing report showing the prevalence of oprL and toxA virulence genes among the clinical isolates of P. aeruginosa. Therefore, this study was conducted for the first time in the country to detect the virulence genes (oprL and toxA) and antibiotic susceptibility pattern of P. aeruginosa. A total of 7,898 clinical specimens were investigated following the standard microbiological procedures. The antibiotic susceptibility testing was examined by the modified disc diffusion method, and virulence genes oprL and toxA of P. aeruginosa were assessed using multiplex PCR. Among the analyzed specimens, 87 isolates were identified to be P. aeruginosa of which 38 (43.68%) isolates were reported as MDR. A higher ratio of P. aeruginosa was detected from urine samples 40 (45.98%), outpatients’ specimens 63 (72.4%), and in patients of the age group of 60–79 years 36 (41.37%). P. aeruginosa was more prevalent in males 56 (64.36%) than in female patients 31 (35.63%). Polymyxin (83.90%) was the most effective antibiotic. P. aeruginosa (100%) isolates harboured the oprL gene, while 95.4% of isolates were positive for the toxA gene. Identification of virulence genes such as oprL and toxA carrying isolates along with the multidrug resistance warrants the need for strategic interventions to prevent the emergence and spread of antimicrobial resistance (AMR). The findings could assist in increasing awareness about antibiotic resistance and suggest the judicious prescription of antibiotics to treat the patients in clinical settings of Nepal. |
| format | Article |
| id | doaj-art-d840a53359134d819b5306d3f96f626f |
| institution | Kabale University |
| issn | 2090-2859 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Emergency Medicine International |
| spelling | doaj-art-d840a53359134d819b5306d3f96f626f2025-02-03T00:58:55ZengWileyEmergency Medicine International2090-28592021-01-01202110.1155/2021/4120697Clinical Specimens are the Pool of Multidrug- resistant Pseudomonas aeruginosa Harbouring oprL and toxA Virulence Genes: Findings from a Tertiary Hospital of NepalYamuna Chand0Sujan Khadka1Sanjeep Sapkota2Suprina Sharma3Santosh Khanal4Alina Thapa5Binod Rayamajhee6Dhruba Kumar Khadka7Om Prakash Panta8Dipendra Shrestha9Pramod Poudel10Department of MicrobiologyState Key Laboratory of Environmental Aquatic ChemistryUniversity of Chinese Academy of SciencesCentral Department of MicrobiologyDepartment of MicrobiologyUniversity of Chinese Academy of SciencesSchool of Optometry and Vision ScienceDepartment of MicrobiologyDepartment of MicrobiologyDepartment of MicrobiologyUniversity Grants CommissionThe multidrug- or extensively drug-resistant (MDR/XDR) Pseudomonas aeruginosa carrying some virulence genes has become a global public health threat. However, in Nepal, there is no existing report showing the prevalence of oprL and toxA virulence genes among the clinical isolates of P. aeruginosa. Therefore, this study was conducted for the first time in the country to detect the virulence genes (oprL and toxA) and antibiotic susceptibility pattern of P. aeruginosa. A total of 7,898 clinical specimens were investigated following the standard microbiological procedures. The antibiotic susceptibility testing was examined by the modified disc diffusion method, and virulence genes oprL and toxA of P. aeruginosa were assessed using multiplex PCR. Among the analyzed specimens, 87 isolates were identified to be P. aeruginosa of which 38 (43.68%) isolates were reported as MDR. A higher ratio of P. aeruginosa was detected from urine samples 40 (45.98%), outpatients’ specimens 63 (72.4%), and in patients of the age group of 60–79 years 36 (41.37%). P. aeruginosa was more prevalent in males 56 (64.36%) than in female patients 31 (35.63%). Polymyxin (83.90%) was the most effective antibiotic. P. aeruginosa (100%) isolates harboured the oprL gene, while 95.4% of isolates were positive for the toxA gene. Identification of virulence genes such as oprL and toxA carrying isolates along with the multidrug resistance warrants the need for strategic interventions to prevent the emergence and spread of antimicrobial resistance (AMR). The findings could assist in increasing awareness about antibiotic resistance and suggest the judicious prescription of antibiotics to treat the patients in clinical settings of Nepal.http://dx.doi.org/10.1155/2021/4120697 |
| spellingShingle | Yamuna Chand Sujan Khadka Sanjeep Sapkota Suprina Sharma Santosh Khanal Alina Thapa Binod Rayamajhee Dhruba Kumar Khadka Om Prakash Panta Dipendra Shrestha Pramod Poudel Clinical Specimens are the Pool of Multidrug- resistant Pseudomonas aeruginosa Harbouring oprL and toxA Virulence Genes: Findings from a Tertiary Hospital of Nepal Emergency Medicine International |
| title | Clinical Specimens are the Pool of Multidrug- resistant Pseudomonas aeruginosa Harbouring oprL and toxA Virulence Genes: Findings from a Tertiary Hospital of Nepal |
| title_full | Clinical Specimens are the Pool of Multidrug- resistant Pseudomonas aeruginosa Harbouring oprL and toxA Virulence Genes: Findings from a Tertiary Hospital of Nepal |
| title_fullStr | Clinical Specimens are the Pool of Multidrug- resistant Pseudomonas aeruginosa Harbouring oprL and toxA Virulence Genes: Findings from a Tertiary Hospital of Nepal |
| title_full_unstemmed | Clinical Specimens are the Pool of Multidrug- resistant Pseudomonas aeruginosa Harbouring oprL and toxA Virulence Genes: Findings from a Tertiary Hospital of Nepal |
| title_short | Clinical Specimens are the Pool of Multidrug- resistant Pseudomonas aeruginosa Harbouring oprL and toxA Virulence Genes: Findings from a Tertiary Hospital of Nepal |
| title_sort | clinical specimens are the pool of multidrug resistant pseudomonas aeruginosa harbouring oprl and toxa virulence genes findings from a tertiary hospital of nepal |
| url | http://dx.doi.org/10.1155/2021/4120697 |
| work_keys_str_mv | AT yamunachand clinicalspecimensarethepoolofmultidrugresistantpseudomonasaeruginosaharbouringoprlandtoxavirulencegenesfindingsfromatertiaryhospitalofnepal AT sujankhadka clinicalspecimensarethepoolofmultidrugresistantpseudomonasaeruginosaharbouringoprlandtoxavirulencegenesfindingsfromatertiaryhospitalofnepal AT sanjeepsapkota clinicalspecimensarethepoolofmultidrugresistantpseudomonasaeruginosaharbouringoprlandtoxavirulencegenesfindingsfromatertiaryhospitalofnepal AT suprinasharma clinicalspecimensarethepoolofmultidrugresistantpseudomonasaeruginosaharbouringoprlandtoxavirulencegenesfindingsfromatertiaryhospitalofnepal AT santoshkhanal clinicalspecimensarethepoolofmultidrugresistantpseudomonasaeruginosaharbouringoprlandtoxavirulencegenesfindingsfromatertiaryhospitalofnepal AT alinathapa clinicalspecimensarethepoolofmultidrugresistantpseudomonasaeruginosaharbouringoprlandtoxavirulencegenesfindingsfromatertiaryhospitalofnepal AT binodrayamajhee clinicalspecimensarethepoolofmultidrugresistantpseudomonasaeruginosaharbouringoprlandtoxavirulencegenesfindingsfromatertiaryhospitalofnepal AT dhrubakumarkhadka clinicalspecimensarethepoolofmultidrugresistantpseudomonasaeruginosaharbouringoprlandtoxavirulencegenesfindingsfromatertiaryhospitalofnepal AT omprakashpanta clinicalspecimensarethepoolofmultidrugresistantpseudomonasaeruginosaharbouringoprlandtoxavirulencegenesfindingsfromatertiaryhospitalofnepal AT dipendrashrestha clinicalspecimensarethepoolofmultidrugresistantpseudomonasaeruginosaharbouringoprlandtoxavirulencegenesfindingsfromatertiaryhospitalofnepal AT pramodpoudel clinicalspecimensarethepoolofmultidrugresistantpseudomonasaeruginosaharbouringoprlandtoxavirulencegenesfindingsfromatertiaryhospitalofnepal |