Isolation and Characterization of <i>Komagataeibacter piraceti</i> sp. nov. and <i>Novacetimonas labruscae</i> sp. nov.: Two Novel Microaerobic Cellulose-Producing Acetic Acid Bacteria from Vinegars
The genera <i>Komagataeibacter</i> and <i>Novacetimonas</i> comprise industrially important species that produce various foods, nanocellulose, acetan-like polysaccharides, enantioselective sugars, and other valuable products. Here, we describe two novel strains, Hr1 and Jurk4...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
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| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/2/456 |
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| Summary: | The genera <i>Komagataeibacter</i> and <i>Novacetimonas</i> comprise industrially important species that produce various foods, nanocellulose, acetan-like polysaccharides, enantioselective sugars, and other valuable products. Here, we describe two novel strains, Hr1 and Jurk4, isolated from pear and apple-grape organic vinegars that showed very high (≥99.39%) 16S rRNA gene sequence identities to species of the <i>Komagataeibacter</i> and <i>Novacetimonas</i> genera, respectively. However, analysis of the 16S-23S rRNA gene internal transcribed spacer (ITS) sequences revealed only 92.6% sequence identity between the Hr1 strain and its closest relative, <i>Komagataeibacter sucrofermentans</i> LMG 18788<sup>T</sup>, and 93.8% sequence identity between the Jurk4 strain and its closest relative, <i>Novacetimonas cocois</i> JCM 31140<sup>T</sup>. Further whole-genome analysis showed for both strains an average nucleotide identity (ANI) below 94% and an in silico DNA–DNA hybridization (dDDH) value of less than 70% to their closest species, supporting their distinction as novel species. The strain Hr1 can be phenotypically differentiated from its closest <i>Komagataeibacter</i> species based on its ability to utilize (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> as the sole nitrogen source in Asai medium with D-glucose and its inability to grow with 1-propanol as a sole carbon source. The strain Jurk4 can be differentiated from other <i>Novacetimonas</i> type strains based on its ability to produce 5-keto-D-gluconic acid, its growth in a medium with glycerol as the sole carbon source, and its inability to grow in an Asai medium with D-glucose. Both strains produce cellulose and possess clusters for acetane-like polysaccharide production, although of different types, which makes them industrially relevant. Based on these findings, we propose <i>Komagataeibacter piraceti</i> sp. nov. Hr1<sup>T</sup> (=ZIM B1167<sup>T</sup> = LMG 33628<sup>T</sup>) and <i>Novacetimonas labruscae</i> sp. nov. Jurk4<sup>T</sup> (=ZIM B1166<sup>T</sup> = LMG 33630<sup>T</sup>) as two novel members of the acetic acid bacteria group. |
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| ISSN: | 2076-2607 |