A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular Tools
Foot-and-mouth disease (FMD) is one of the highly contagious diseases of domestic animals. Effective control of this disease needs sensitive, specific, and quick diagnostic tools at each tier of control strategy. In this paper we have outlined various diagnostic approaches from old to new generation...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.4061/2011/905768 |
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| author | Neeta Longjam Rajib Deb A. K. Sarmah Tilling Tayo V. B. Awachat V. K. Saxena |
| author_facet | Neeta Longjam Rajib Deb A. K. Sarmah Tilling Tayo V. B. Awachat V. K. Saxena |
| author_sort | Neeta Longjam |
| collection | DOAJ |
| description | Foot-and-mouth disease (FMD) is one of the highly contagious diseases of domestic animals. Effective control of this disease needs sensitive, specific, and quick diagnostic tools at each tier of control strategy. In this paper we have outlined various diagnostic approaches from old to new generation in a nutshell. Presently FMD diagnosis is being carried out using techniques such as Virus Isolation (VI), Sandwich-ELISA (S-ELISA), Liquid-Phase Blocking ELISA (LPBE), Multiplex-PCR (m-PCR), and indirect ELISA (DIVA), and real time-PCR can be used for detection of antibody against nonstructural proteins. Nucleotide sequencing for serotyping, microarray as well as recombinant antigen-based detection, biosensor, phage display, and nucleic-acid-based diagnostic are on the way for rapid and specific detection of FMDV. Various pen side tests, namely, lateral flow, RT-LAMP, Immunostrip tests, and so forth. are also developed for detection of the virus in field condition. |
| format | Article |
| id | doaj-art-d82749d3bc214b5f897a313ac8af0e79 |
| institution | Kabale University |
| issn | 2042-0048 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-d82749d3bc214b5f897a313ac8af0e792025-02-03T01:26:48ZengWileyVeterinary Medicine International2042-00482011-01-01201110.4061/2011/905768905768A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular ToolsNeeta Longjam0Rajib Deb1A. K. Sarmah2Tilling Tayo3V. B. Awachat4V. K. Saxena5Department of Veterinary Microbiology, College of Veterinary Science, Assam Agricultural University, Guwahati 781022, IndiaDivision of Animal Biotechnology, Indian Veterinary Research Institute (IVRI), Izatnagar 243122, IndiaDepartment of Veterinary Microbiology, College of Veterinary Science, Assam Agricultural University, Guwahati 781022, IndiaDivision of Animal Nutrition, Indian Veterinary Research Institute (IVRI), Izatnagar 243122, IndiaDivision of Poultry Science, Central Avian Research Institute (CARI), Izatnagar 243122, IndiaDivision of Veterinary Biochemistry and Physiology, Central Sheep and Wool Research Institute (CSWRI), Avikanagar, IndiaFoot-and-mouth disease (FMD) is one of the highly contagious diseases of domestic animals. Effective control of this disease needs sensitive, specific, and quick diagnostic tools at each tier of control strategy. In this paper we have outlined various diagnostic approaches from old to new generation in a nutshell. Presently FMD diagnosis is being carried out using techniques such as Virus Isolation (VI), Sandwich-ELISA (S-ELISA), Liquid-Phase Blocking ELISA (LPBE), Multiplex-PCR (m-PCR), and indirect ELISA (DIVA), and real time-PCR can be used for detection of antibody against nonstructural proteins. Nucleotide sequencing for serotyping, microarray as well as recombinant antigen-based detection, biosensor, phage display, and nucleic-acid-based diagnostic are on the way for rapid and specific detection of FMDV. Various pen side tests, namely, lateral flow, RT-LAMP, Immunostrip tests, and so forth. are also developed for detection of the virus in field condition.http://dx.doi.org/10.4061/2011/905768 |
| spellingShingle | Neeta Longjam Rajib Deb A. K. Sarmah Tilling Tayo V. B. Awachat V. K. Saxena A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular Tools Veterinary Medicine International |
| title | A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular Tools |
| title_full | A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular Tools |
| title_fullStr | A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular Tools |
| title_full_unstemmed | A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular Tools |
| title_short | A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular Tools |
| title_sort | brief review on diagnosis of foot and mouth disease of livestock conventional to molecular tools |
| url | http://dx.doi.org/10.4061/2011/905768 |
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