Process optimization studies on xylanase production and bioethanol fermentation
Xylanase is one of the hydrolytic enzymes with a broad industrial application in several industries. Bioethanol can be synthesised from lignocellulosic biomass by using xylanase and other hydrolytic enzymes. A filamentous fungus, which is Aspergillus niger produces xylanase under submerged fermentat...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2025-01-01
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| Series: | E3S Web of Conferences |
| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2025/03/e3sconf_isgst2024_03005.pdf |
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| author | Chia Zhi Tan Thien Sze Chen Ivory Yi Peng Teoh Kok Chang Lee Balakrishnan Kunasundari Xian Ooi Zhong |
| author_facet | Chia Zhi Tan Thien Sze Chen Ivory Yi Peng Teoh Kok Chang Lee Balakrishnan Kunasundari Xian Ooi Zhong |
| author_sort | Chia Zhi Tan |
| collection | DOAJ |
| description | Xylanase is one of the hydrolytic enzymes with a broad industrial application in several industries. Bioethanol can be synthesised from lignocellulosic biomass by using xylanase and other hydrolytic enzymes. A filamentous fungus, which is Aspergillus niger produces xylanase under submerged fermentation when oil palm empty fruit brunches were used as carbon sources. This study aimed to optimize the operating conditions (medium pH and incubation temperature) of xylanase production process using the OFAT analysis technique. From the data obtained, the highest xylanase production was 0.508 U/mL at pH 5.0 and 0.524 U/mL at an incubation temperature of 32°C, respectively. S. cerevisiae yeast was added into the fermentation supernatant for bioethanol fermentation. The concentration of bioethanol produced by xylanase enzyme from A. niger at optimum operating condition was 15.54±0.47 g/L. This study proved that A. niger is one of the filamentous fungi which show the potential of hydrolysing lignocellulosic material to carbon sources and subsequently to bioethanol production. |
| format | Article |
| id | doaj-art-d80d2e003e10431fa63cbbfc1f4986a1 |
| institution | Kabale University |
| issn | 2267-1242 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj-art-d80d2e003e10431fa63cbbfc1f4986a12025-02-05T10:47:33ZengEDP SciencesE3S Web of Conferences2267-12422025-01-016030300510.1051/e3sconf/202560303005e3sconf_isgst2024_03005Process optimization studies on xylanase production and bioethanol fermentationChia Zhi Tan0Thien Sze Chen Ivory1Yi Peng Teoh2Kok Chang Lee3Balakrishnan Kunasundari4Xian Ooi Zhong5Department of Petrochemical Engineering, Faculty of Engineering and Green Technology, Universiti Tunku Abdul Rahman (UTAR), Kampar Campus, Jalan Universiti, Bandar BaratDepartment of Petrochemical Engineering, Faculty of Engineering and Green Technology, Universiti Tunku Abdul Rahman (UTAR), Kampar Campus, Jalan Universiti, Bandar BaratDepartment of Petrochemical Engineering, Faculty of Engineering and Green Technology, Universiti Tunku Abdul Rahman (UTAR), Kampar Campus, Jalan Universiti, Bandar BaratDepartment of Biological Science, Faculty of Science, Universiti Tunku Abdul Rahman (UTAR), Kampar Campus, Jalan Universiti, Bandar BaratDepartment of Chemical Engineering Technology, Faculty of Engineering Technology, Universiti Malaysia Perlis, Kampus UniCITI Alam, Sungai ChuchuhDepartment of Chemical Science, Faculty of Science, Universiti Tunku Abdul Rahman (UTAR), Kampar Campus, Jalan Universiti, Bandar BaratXylanase is one of the hydrolytic enzymes with a broad industrial application in several industries. Bioethanol can be synthesised from lignocellulosic biomass by using xylanase and other hydrolytic enzymes. A filamentous fungus, which is Aspergillus niger produces xylanase under submerged fermentation when oil palm empty fruit brunches were used as carbon sources. This study aimed to optimize the operating conditions (medium pH and incubation temperature) of xylanase production process using the OFAT analysis technique. From the data obtained, the highest xylanase production was 0.508 U/mL at pH 5.0 and 0.524 U/mL at an incubation temperature of 32°C, respectively. S. cerevisiae yeast was added into the fermentation supernatant for bioethanol fermentation. The concentration of bioethanol produced by xylanase enzyme from A. niger at optimum operating condition was 15.54±0.47 g/L. This study proved that A. niger is one of the filamentous fungi which show the potential of hydrolysing lignocellulosic material to carbon sources and subsequently to bioethanol production.https://www.e3s-conferences.org/articles/e3sconf/pdf/2025/03/e3sconf_isgst2024_03005.pdf |
| spellingShingle | Chia Zhi Tan Thien Sze Chen Ivory Yi Peng Teoh Kok Chang Lee Balakrishnan Kunasundari Xian Ooi Zhong Process optimization studies on xylanase production and bioethanol fermentation E3S Web of Conferences |
| title | Process optimization studies on xylanase production and bioethanol fermentation |
| title_full | Process optimization studies on xylanase production and bioethanol fermentation |
| title_fullStr | Process optimization studies on xylanase production and bioethanol fermentation |
| title_full_unstemmed | Process optimization studies on xylanase production and bioethanol fermentation |
| title_short | Process optimization studies on xylanase production and bioethanol fermentation |
| title_sort | process optimization studies on xylanase production and bioethanol fermentation |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2025/03/e3sconf_isgst2024_03005.pdf |
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