EFFECT OF PLANT-GROWTH REGULATORS ON THE FORMATION OF Begonia bataiensis CALLUS
Leaves and young petiole of Begonia bataiensis were sterilized with 10% calcium hypochlorite (Ca(OCl)2) and indurations of 0, 4, 6, 8, 10, and 12 minutes. Then they were plated on media consisting of a half dish of MS supplemented by 30 g/l sucrose and 7.5 g/l agar. After 21 days, the culturing rate...
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Dalat University
2018-09-01
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| Series: | Tạp chí Khoa học Đại học Đà Lạt |
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| Online Access: | http://tckh.dlu.edu.vn/index.php/tckhdhdl/article/view/438 |
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| author | Đinh Văn Khiêm Nguyễn Thị Thu Hậu |
| author_facet | Đinh Văn Khiêm Nguyễn Thị Thu Hậu |
| author_sort | Đinh Văn Khiêm |
| collection | DOAJ |
| description | Leaves and young petiole of Begonia bataiensis were sterilized with 10% calcium hypochlorite (Ca(OCl)2) and indurations of 0, 4, 6, 8, 10, and 12 minutes. Then they were plated on media consisting of a half dish of MS supplemented by 30 g/l sucrose and 7.5 g/l agar. After 21 days, the culturing rate of clean samples was 81.7%. Leaves and stems of the transplanted plants in vitro were cultured on a half dish of MS media supplemented with 30g/l sucrose, 7.5g/l agar and plant growth-regulators: 2.4-Dichlorophenoxyacetic acid (2.4D) and thidiazuron (TDZ). Various concentration of TDZ (0; 0.1; 0.3; 0.5; 1 mg/l) were used. After 21 days, results were obtained for 96% of the callus on the medium supplemented with 0.3 mg/l TDZ. The callus had a fresh weight of 2,642 mg/sample and a dry weight of 271 mg/sample. The calluses were transferred onto a medium for forming shoots and roots to complete the plant in vitro. |
| format | Article |
| id | doaj-art-d802458fdb2343c3a7c3333f5bbf007e |
| institution | Kabale University |
| issn | 0866-787X 0866-787X |
| language | English |
| publishDate | 2018-09-01 |
| publisher | Dalat University |
| record_format | Article |
| series | Tạp chí Khoa học Đại học Đà Lạt |
| spelling | doaj-art-d802458fdb2343c3a7c3333f5bbf007e2025-02-02T05:41:26ZengDalat UniversityTạp chí Khoa học Đại học Đà Lạt0866-787X0866-787X2018-09-0183697610.37569/DalatUniversity.8.3.438(2018)258EFFECT OF PLANT-GROWTH REGULATORS ON THE FORMATION OF Begonia bataiensis CALLUSĐinh Văn Khiêm0Nguyễn Thị Thu Hậu1Viện Nghiên cứu Khoa học Tây NguyênKhoa Nông nghiệp và Phát triển nông thôn, Trường Đại học Kiên GiangLeaves and young petiole of Begonia bataiensis were sterilized with 10% calcium hypochlorite (Ca(OCl)2) and indurations of 0, 4, 6, 8, 10, and 12 minutes. Then they were plated on media consisting of a half dish of MS supplemented by 30 g/l sucrose and 7.5 g/l agar. After 21 days, the culturing rate of clean samples was 81.7%. Leaves and stems of the transplanted plants in vitro were cultured on a half dish of MS media supplemented with 30g/l sucrose, 7.5g/l agar and plant growth-regulators: 2.4-Dichlorophenoxyacetic acid (2.4D) and thidiazuron (TDZ). Various concentration of TDZ (0; 0.1; 0.3; 0.5; 1 mg/l) were used. After 21 days, results were obtained for 96% of the callus on the medium supplemented with 0.3 mg/l TDZ. The callus had a fresh weight of 2,642 mg/sample and a dry weight of 271 mg/sample. The calluses were transferred onto a medium for forming shoots and roots to complete the plant in vitro.http://tckh.dlu.edu.vn/index.php/tckhdhdl/article/view/438cây thu hải đường bataiensis (begonia bataiensis)chất kích thích sinh trưởng thực vậtin vitromô sẹo. |
| spellingShingle | Đinh Văn Khiêm Nguyễn Thị Thu Hậu EFFECT OF PLANT-GROWTH REGULATORS ON THE FORMATION OF Begonia bataiensis CALLUS Tạp chí Khoa học Đại học Đà Lạt cây thu hải đường bataiensis (begonia bataiensis) chất kích thích sinh trưởng thực vật in vitro mô sẹo. |
| title | EFFECT OF PLANT-GROWTH REGULATORS ON THE FORMATION OF Begonia bataiensis CALLUS |
| title_full | EFFECT OF PLANT-GROWTH REGULATORS ON THE FORMATION OF Begonia bataiensis CALLUS |
| title_fullStr | EFFECT OF PLANT-GROWTH REGULATORS ON THE FORMATION OF Begonia bataiensis CALLUS |
| title_full_unstemmed | EFFECT OF PLANT-GROWTH REGULATORS ON THE FORMATION OF Begonia bataiensis CALLUS |
| title_short | EFFECT OF PLANT-GROWTH REGULATORS ON THE FORMATION OF Begonia bataiensis CALLUS |
| title_sort | effect of plant growth regulators on the formation of begonia bataiensis callus |
| topic | cây thu hải đường bataiensis (begonia bataiensis) chất kích thích sinh trưởng thực vật in vitro mô sẹo. |
| url | http://tckh.dlu.edu.vn/index.php/tckhdhdl/article/view/438 |
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