EFFECT OF PLANT-GROWTH REGULATORS ON THE FORMATION OF Begonia bataiensis CALLUS
Leaves and young petiole of Begonia bataiensis were sterilized with 10% calcium hypochlorite (Ca(OCl)2) and indurations of 0, 4, 6, 8, 10, and 12 minutes. Then they were plated on media consisting of a half dish of MS supplemented by 30 g/l sucrose and 7.5 g/l agar. After 21 days, the culturing rate...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Dalat University
2018-09-01
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| Series: | Tạp chí Khoa học Đại học Đà Lạt |
| Subjects: | |
| Online Access: | http://tckh.dlu.edu.vn/index.php/tckhdhdl/article/view/438 |
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| Summary: | Leaves and young petiole of Begonia bataiensis were sterilized with 10% calcium hypochlorite (Ca(OCl)2) and indurations of 0, 4, 6, 8, 10, and 12 minutes. Then they were plated on media consisting of a half dish of MS supplemented by 30 g/l sucrose and 7.5 g/l agar. After 21 days, the culturing rate of clean samples was 81.7%. Leaves and stems of the transplanted plants in vitro were cultured on a half dish of MS media supplemented with 30g/l sucrose, 7.5g/l agar and plant growth-regulators: 2.4-Dichlorophenoxyacetic acid (2.4D) and thidiazuron (TDZ). Various concentration of TDZ (0; 0.1; 0.3; 0.5; 1 mg/l) were used. After 21 days, results were obtained for 96% of the callus on the medium supplemented with 0.3 mg/l TDZ. The callus had a fresh weight of 2,642 mg/sample and a dry weight of 271 mg/sample. The calluses were transferred onto a medium for forming shoots and roots to complete the plant in vitro. |
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| ISSN: | 0866-787X 0866-787X |