Morphological and Molecular Characterization of a New Isospora (Apicomplexa: Eimeriidae) Species From a Singing Honeyeater (Gavicalis virescens Vieillot, 1817) (Passeriformes: Meliphagidae) in Western Australia
ABSTRACT We describe and characterize a new Isospora species from the Singing honeyeater (Gavicalis virescens) in Western Australia, both morphologically and molecularly. Microscopic analysis of a fecal sample identified 25 ellipsoidal oocysts, measuring 21–25 × 18–20 μm (mean 23.4 × 18.7 μm), with...
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Wiley
2025-01-01
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| Series: | Ecology and Evolution |
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| Online Access: | https://doi.org/10.1002/ece3.70801 |
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| author | Yinhua Chen Belinda Brice Bruno P. Berto Rongchang Yang |
| author_facet | Yinhua Chen Belinda Brice Bruno P. Berto Rongchang Yang |
| author_sort | Yinhua Chen |
| collection | DOAJ |
| description | ABSTRACT We describe and characterize a new Isospora species from the Singing honeyeater (Gavicalis virescens) in Western Australia, both morphologically and molecularly. Microscopic analysis of a fecal sample identified 25 ellipsoidal oocysts, measuring 21–25 × 18–20 μm (mean 23.4 × 18.7 μm), with a length/width (L/W) ratio of 1.2–1.3 (mean 1.25). The oocyst wall is bilayered and ~1.0 μm thick, with a smooth outer layer making up about two‐thirds of the thickness. A micropyle cap, measuring ~2.0 × 4.5 μm, is present as a curved protrusion on the outer layer. The micropyle itself is ~4.5 μm wide. The oocysts lack a residuum but contain 1–2 polar granules. The 25 ellipsoidal sporocysts measured 14–15 × 8–9 μm (mean 14.1 × 8.7 μm) with an L/W ratio of 1.6–1.7 (mean 1.62). The Stieda body is flattened (~0.5 × 1.5 μm), the sub‐Stieda body is rounded (~1.5 × 2.5 μm), and the para‐Stieda body is absent. The sporocyst residuum, composed of clustered spherules, is ~3.5 × 3.5 μm. Sporozoites contain anterior and posterior refractile bodies and a nucleus. Molecular analysis of the 18S rRNA, 28S rRNA, and COI gene loci showed a 99.5% genetic similarity to Isospora neochmiae at the 18S rRNA locus and 99.2% and 97.7% similarities to Isospora manorinae at the 28S rRNA and COI loci, respectively. Phylogenetic analysis confirmed that the new species is closely related to I. manorinae. Based on these data, we propose this isolate as a new species, Isospora virescensae n. sp. |
| format | Article |
| id | doaj-art-d7f279a5c25c450dbc2ee1ce677971c2 |
| institution | Kabale University |
| issn | 2045-7758 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
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| series | Ecology and Evolution |
| spelling | doaj-art-d7f279a5c25c450dbc2ee1ce677971c22025-01-29T05:08:42ZengWileyEcology and Evolution2045-77582025-01-01151n/an/a10.1002/ece3.70801Morphological and Molecular Characterization of a New Isospora (Apicomplexa: Eimeriidae) Species From a Singing Honeyeater (Gavicalis virescens Vieillot, 1817) (Passeriformes: Meliphagidae) in Western AustraliaYinhua Chen0Belinda Brice1Bruno P. Berto2Rongchang Yang3Department of Resources and Environmental Sciences Maotai College Renhuai Guizhou ChinaKanyana Wildlife Rehabilitation Centre Lesmurdie Western Australia AustraliaDepartamento de Biologia Animal, Instituto de Ciências Biológicas e da Saúde Universidade Federal Rural do Rio de Janeiro Seropédica Rio de Janeiro BrazilSchool of Agricultural Sciences Murdoch University Murdoch Western Australia AustraliaABSTRACT We describe and characterize a new Isospora species from the Singing honeyeater (Gavicalis virescens) in Western Australia, both morphologically and molecularly. Microscopic analysis of a fecal sample identified 25 ellipsoidal oocysts, measuring 21–25 × 18–20 μm (mean 23.4 × 18.7 μm), with a length/width (L/W) ratio of 1.2–1.3 (mean 1.25). The oocyst wall is bilayered and ~1.0 μm thick, with a smooth outer layer making up about two‐thirds of the thickness. A micropyle cap, measuring ~2.0 × 4.5 μm, is present as a curved protrusion on the outer layer. The micropyle itself is ~4.5 μm wide. The oocysts lack a residuum but contain 1–2 polar granules. The 25 ellipsoidal sporocysts measured 14–15 × 8–9 μm (mean 14.1 × 8.7 μm) with an L/W ratio of 1.6–1.7 (mean 1.62). The Stieda body is flattened (~0.5 × 1.5 μm), the sub‐Stieda body is rounded (~1.5 × 2.5 μm), and the para‐Stieda body is absent. The sporocyst residuum, composed of clustered spherules, is ~3.5 × 3.5 μm. Sporozoites contain anterior and posterior refractile bodies and a nucleus. Molecular analysis of the 18S rRNA, 28S rRNA, and COI gene loci showed a 99.5% genetic similarity to Isospora neochmiae at the 18S rRNA locus and 99.2% and 97.7% similarities to Isospora manorinae at the 28S rRNA and COI loci, respectively. Phylogenetic analysis confirmed that the new species is closely related to I. manorinae. Based on these data, we propose this isolate as a new species, Isospora virescensae n. sp.https://doi.org/10.1002/ece3.7080118S rRNA28S rRNACoccidiaCOI geneIsosporaSinging honeyeater |
| spellingShingle | Yinhua Chen Belinda Brice Bruno P. Berto Rongchang Yang Morphological and Molecular Characterization of a New Isospora (Apicomplexa: Eimeriidae) Species From a Singing Honeyeater (Gavicalis virescens Vieillot, 1817) (Passeriformes: Meliphagidae) in Western Australia Ecology and Evolution 18S rRNA 28S rRNA Coccidia COI gene Isospora Singing honeyeater |
| title | Morphological and Molecular Characterization of a New Isospora (Apicomplexa: Eimeriidae) Species From a Singing Honeyeater (Gavicalis virescens Vieillot, 1817) (Passeriformes: Meliphagidae) in Western Australia |
| title_full | Morphological and Molecular Characterization of a New Isospora (Apicomplexa: Eimeriidae) Species From a Singing Honeyeater (Gavicalis virescens Vieillot, 1817) (Passeriformes: Meliphagidae) in Western Australia |
| title_fullStr | Morphological and Molecular Characterization of a New Isospora (Apicomplexa: Eimeriidae) Species From a Singing Honeyeater (Gavicalis virescens Vieillot, 1817) (Passeriformes: Meliphagidae) in Western Australia |
| title_full_unstemmed | Morphological and Molecular Characterization of a New Isospora (Apicomplexa: Eimeriidae) Species From a Singing Honeyeater (Gavicalis virescens Vieillot, 1817) (Passeriformes: Meliphagidae) in Western Australia |
| title_short | Morphological and Molecular Characterization of a New Isospora (Apicomplexa: Eimeriidae) Species From a Singing Honeyeater (Gavicalis virescens Vieillot, 1817) (Passeriformes: Meliphagidae) in Western Australia |
| title_sort | morphological and molecular characterization of a new isospora apicomplexa eimeriidae species from a singing honeyeater gavicalis virescens vieillot 1817 passeriformes meliphagidae in western australia |
| topic | 18S rRNA 28S rRNA Coccidia COI gene Isospora Singing honeyeater |
| url | https://doi.org/10.1002/ece3.70801 |
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