HAVCR1 Affects the MEK/ERK Pathway in Gastric Adenocarcinomas and Influences Tumor Progression and Patient Outcome
The hepatitis A virus cellular receptor 1 (HAVCR1) gene as a sensitive and specific biomarker has been reported in various diseases. Especially, HAVCR1 overexpression promotes the development and progression of several human cancers. Hence, we aimed to detect the effects of HAVCR1 on gastric adenoca...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2019/6746970 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832563807278333952 |
|---|---|
| author | Ji Xue Ying Li Jianfeng Yi Hong Jiang |
| author_facet | Ji Xue Ying Li Jianfeng Yi Hong Jiang |
| author_sort | Ji Xue |
| collection | DOAJ |
| description | The hepatitis A virus cellular receptor 1 (HAVCR1) gene as a sensitive and specific biomarker has been reported in various diseases. Especially, HAVCR1 overexpression promotes the development and progression of several human cancers. Hence, we aimed to detect the effects of HAVCR1 on gastric adenocarcinoma (GAC). We first determined the expression of HAVCR1 in GAC tissues compared with normal gastric tissues based on the Cancer Genome Atlas (TCGA) database using bioinformatics analysis methods. Then, we assessed the biological function of HAVCR1 in GAC cells using quantitative real-time reverse transcription-PCR (qRT-PCR), western blot, cell counting kit-8- (CCK-) 8, colony formation assay, wound healing assay, and transwell assay. Our results showed that HAVCR1 expression was upregulated in GAC tissues and positively associated with poor survival. Loss-of-function analyses indicated that knockdown of HAVCR1 inhibited the proliferation, colony formation, migration, and invasion of GAC cells. Furthermore, reduction of HAVCR1 in GAC cells can decrease the expression of phosphorylated MEK/ERK. These findings suggested that HAVCR1 may represent a potential biomarker for GAC prognosis, as well as a novel therapeutic target for GAC treatment. |
| format | Article |
| id | doaj-art-d7eb9d4664a04b67a5253836324908a7 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-d7eb9d4664a04b67a5253836324908a72025-02-03T01:12:29ZengWileyGastroenterology Research and Practice1687-61211687-630X2019-01-01201910.1155/2019/67469706746970HAVCR1 Affects the MEK/ERK Pathway in Gastric Adenocarcinomas and Influences Tumor Progression and Patient OutcomeJi Xue0Ying Li1Jianfeng Yi2Hong Jiang3Department of Traditional Chinese Medicine, The Second Hospital of Jilin University, No. 218 Nanguan District, Changchun, Jilin 130041, ChinaBeijing Super Link Medical Research Institute, Beokomg, Fengtai District, Caihuying No. 58, Room 1102, Beijing 100000, ChinaDepartment of Surgery, School of Medicine, Gansu University of Chinese Medicine, No. 35 Dingxi East Road, Lanzhou, Gansu 730000, ChinaDepartment of Surgery, Xi’an Hospital of Traditional Chinese Medicine, No. 69 Fengcheng, 8th Road, Xi’an, Shanxi 710021, ChinaThe hepatitis A virus cellular receptor 1 (HAVCR1) gene as a sensitive and specific biomarker has been reported in various diseases. Especially, HAVCR1 overexpression promotes the development and progression of several human cancers. Hence, we aimed to detect the effects of HAVCR1 on gastric adenocarcinoma (GAC). We first determined the expression of HAVCR1 in GAC tissues compared with normal gastric tissues based on the Cancer Genome Atlas (TCGA) database using bioinformatics analysis methods. Then, we assessed the biological function of HAVCR1 in GAC cells using quantitative real-time reverse transcription-PCR (qRT-PCR), western blot, cell counting kit-8- (CCK-) 8, colony formation assay, wound healing assay, and transwell assay. Our results showed that HAVCR1 expression was upregulated in GAC tissues and positively associated with poor survival. Loss-of-function analyses indicated that knockdown of HAVCR1 inhibited the proliferation, colony formation, migration, and invasion of GAC cells. Furthermore, reduction of HAVCR1 in GAC cells can decrease the expression of phosphorylated MEK/ERK. These findings suggested that HAVCR1 may represent a potential biomarker for GAC prognosis, as well as a novel therapeutic target for GAC treatment.http://dx.doi.org/10.1155/2019/6746970 |
| spellingShingle | Ji Xue Ying Li Jianfeng Yi Hong Jiang HAVCR1 Affects the MEK/ERK Pathway in Gastric Adenocarcinomas and Influences Tumor Progression and Patient Outcome Gastroenterology Research and Practice |
| title | HAVCR1 Affects the MEK/ERK Pathway in Gastric Adenocarcinomas and Influences Tumor Progression and Patient Outcome |
| title_full | HAVCR1 Affects the MEK/ERK Pathway in Gastric Adenocarcinomas and Influences Tumor Progression and Patient Outcome |
| title_fullStr | HAVCR1 Affects the MEK/ERK Pathway in Gastric Adenocarcinomas and Influences Tumor Progression and Patient Outcome |
| title_full_unstemmed | HAVCR1 Affects the MEK/ERK Pathway in Gastric Adenocarcinomas and Influences Tumor Progression and Patient Outcome |
| title_short | HAVCR1 Affects the MEK/ERK Pathway in Gastric Adenocarcinomas and Influences Tumor Progression and Patient Outcome |
| title_sort | havcr1 affects the mek erk pathway in gastric adenocarcinomas and influences tumor progression and patient outcome |
| url | http://dx.doi.org/10.1155/2019/6746970 |
| work_keys_str_mv | AT jixue havcr1affectsthemekerkpathwayingastricadenocarcinomasandinfluencestumorprogressionandpatientoutcome AT yingli havcr1affectsthemekerkpathwayingastricadenocarcinomasandinfluencestumorprogressionandpatientoutcome AT jianfengyi havcr1affectsthemekerkpathwayingastricadenocarcinomasandinfluencestumorprogressionandpatientoutcome AT hongjiang havcr1affectsthemekerkpathwayingastricadenocarcinomasandinfluencestumorprogressionandpatientoutcome |