Identification of RNA Editing Sites Reveals Functional Modifications with the Addition of Methionine to the Daily Rations of Yaks
Methionine is an amino acid necessary for the growth and development of all animals. Glutathione produced during methionine metabolism can reduce damage to cells caused by oxidative stress. Supplementing restricted amino acids in animals by scientific means will be beneficial to protein synthesis, w...
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2025-01-01
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| author | Shiyu Wu Xinrui Liu Yaxin Liu Shikai Wang Wei Peng Ming Zhang Binglin Yue Hui Wang Jikun Wang Jincheng Zhong Fang Sun Yixi Kangzhu Jiabo Wang |
| author_facet | Shiyu Wu Xinrui Liu Yaxin Liu Shikai Wang Wei Peng Ming Zhang Binglin Yue Hui Wang Jikun Wang Jincheng Zhong Fang Sun Yixi Kangzhu Jiabo Wang |
| author_sort | Shiyu Wu |
| collection | DOAJ |
| description | Methionine is an amino acid necessary for the growth and development of all animals. Glutathione produced during methionine metabolism can reduce damage to cells caused by oxidative stress. Supplementing restricted amino acids in animals by scientific means will be beneficial to protein synthesis, which will affect the growth and development of animals and will bring huge economic benefits when applied to actual production and life. In this study, we collected three muscle tissues from 24 male Maiwa yaks, which were fattened for three months with different methionine concentrations in their diet. RNA-seq was performed to obtain expression reads. A total of 1116 editing sites were identified by at least two software; the editing site types were mainly T-to-C and A-to-G mutations. We found two significant RNA editing sites presenting high-risk editing types. One was located on the <i>MSRA</i> gene that regulates the reduction of methionine, and the other can make changes to the properties of encoded proteins. This provides further understanding of the mechanism of yak muscle tissue and regulation of gene expression after the addition of methionine to daily rations. |
| format | Article |
| id | doaj-art-d7dd8e31aac24b9a87078a895aab9021 |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-d7dd8e31aac24b9a87078a895aab90212025-01-24T13:17:53ZengMDPI AGAnimals2076-26152025-01-0115217110.3390/ani15020171Identification of RNA Editing Sites Reveals Functional Modifications with the Addition of Methionine to the Daily Rations of YaksShiyu Wu0Xinrui Liu1Yaxin Liu2Shikai Wang3Wei Peng4Ming Zhang5Binglin Yue6Hui Wang7Jikun Wang8Jincheng Zhong9Fang Sun10Yixi Kangzhu11Jiabo Wang12Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610225, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610225, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610225, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610225, ChinaQinghai Academy of Animal Science and Veterinary Science, Qinghai University, Xining 810016, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610225, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610225, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610225, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610225, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610225, ChinaKey Laboratory of Combining Farming and Animal Husbandry, Ministry of Agriculture, Institute of Animal-Husbandry, Heilongjiang Academy of Agricultural Sciences, Harbin 150028, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610225, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610225, ChinaMethionine is an amino acid necessary for the growth and development of all animals. Glutathione produced during methionine metabolism can reduce damage to cells caused by oxidative stress. Supplementing restricted amino acids in animals by scientific means will be beneficial to protein synthesis, which will affect the growth and development of animals and will bring huge economic benefits when applied to actual production and life. In this study, we collected three muscle tissues from 24 male Maiwa yaks, which were fattened for three months with different methionine concentrations in their diet. RNA-seq was performed to obtain expression reads. A total of 1116 editing sites were identified by at least two software; the editing site types were mainly T-to-C and A-to-G mutations. We found two significant RNA editing sites presenting high-risk editing types. One was located on the <i>MSRA</i> gene that regulates the reduction of methionine, and the other can make changes to the properties of encoded proteins. This provides further understanding of the mechanism of yak muscle tissue and regulation of gene expression after the addition of methionine to daily rations.https://www.mdpi.com/2076-2615/15/2/171<i>Bos grunniens</i>RNA-seqfattenJACUSASPRINTREDItools |
| spellingShingle | Shiyu Wu Xinrui Liu Yaxin Liu Shikai Wang Wei Peng Ming Zhang Binglin Yue Hui Wang Jikun Wang Jincheng Zhong Fang Sun Yixi Kangzhu Jiabo Wang Identification of RNA Editing Sites Reveals Functional Modifications with the Addition of Methionine to the Daily Rations of Yaks Animals <i>Bos grunniens</i> RNA-seq fatten JACUSA SPRINT REDItools |
| title | Identification of RNA Editing Sites Reveals Functional Modifications with the Addition of Methionine to the Daily Rations of Yaks |
| title_full | Identification of RNA Editing Sites Reveals Functional Modifications with the Addition of Methionine to the Daily Rations of Yaks |
| title_fullStr | Identification of RNA Editing Sites Reveals Functional Modifications with the Addition of Methionine to the Daily Rations of Yaks |
| title_full_unstemmed | Identification of RNA Editing Sites Reveals Functional Modifications with the Addition of Methionine to the Daily Rations of Yaks |
| title_short | Identification of RNA Editing Sites Reveals Functional Modifications with the Addition of Methionine to the Daily Rations of Yaks |
| title_sort | identification of rna editing sites reveals functional modifications with the addition of methionine to the daily rations of yaks |
| topic | <i>Bos grunniens</i> RNA-seq fatten JACUSA SPRINT REDItools |
| url | https://www.mdpi.com/2076-2615/15/2/171 |
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