Development and Validation of a Derivative Spectrophotometric Method for Simultaneous Determination of Simvastatin and Ezetimibe
A combination of simvastatin and ezetimibe with complementary mechanisms of action is used for treating high levels of cholesterol in the blood. The aim of this study was to develop a rapid and sensitive derivative spectrophotometric method for analysis of these drugs in combined dosage forms. A fir...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2010/423057 |
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| author | Effat Souri Masoud Amanlou |
| author_facet | Effat Souri Masoud Amanlou |
| author_sort | Effat Souri |
| collection | DOAJ |
| description | A combination of simvastatin and ezetimibe with complementary mechanisms of action is used for treating high levels of cholesterol in the blood. The aim of this study was to develop a rapid and sensitive derivative spectrophotometric method for analysis of these drugs in combined dosage forms. A first order derivative spectrophotometric method was developed for simultaneous determination of simvastatin and ezetimibe using zero-crossing technique. The measurements were carried out at 219 and 265 nm for simvastatin and ezetimibe respectively. The described method was found to be linear (r2>0.999) over the range of 2-40 μg/mL for simvastatin in the presence of 10 μg/mL ezetimibe at 219 nm and in the range of 1-20 μg/mL of ezetimibe in the presence of 20 μg/mL of simvastatin at 265 nm. The within-day and between-day precision values for both drugs were less than 3% (CV). Also, good recoveries were obtained with both synthetic mixtures and commercial tablets. The proposed method was successfully applied for simultaneous determination of simvastatin and ezetimibe in a pharmaceutical dosage form without any interference from excipients. |
| format | Article |
| id | doaj-art-d7d9430aa6b5482087a1917bc3e65568 |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-d7d9430aa6b5482087a1917bc3e655682025-02-03T01:27:51ZengWileyE-Journal of Chemistry0973-49452090-98102010-01-017S1S197S20210.1155/2010/423057Development and Validation of a Derivative Spectrophotometric Method for Simultaneous Determination of Simvastatin and EzetimibeEffat Souri0Masoud Amanlou1Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran (14155-6451), IranDepartment of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran (14155-6451), IranA combination of simvastatin and ezetimibe with complementary mechanisms of action is used for treating high levels of cholesterol in the blood. The aim of this study was to develop a rapid and sensitive derivative spectrophotometric method for analysis of these drugs in combined dosage forms. A first order derivative spectrophotometric method was developed for simultaneous determination of simvastatin and ezetimibe using zero-crossing technique. The measurements were carried out at 219 and 265 nm for simvastatin and ezetimibe respectively. The described method was found to be linear (r2>0.999) over the range of 2-40 μg/mL for simvastatin in the presence of 10 μg/mL ezetimibe at 219 nm and in the range of 1-20 μg/mL of ezetimibe in the presence of 20 μg/mL of simvastatin at 265 nm. The within-day and between-day precision values for both drugs were less than 3% (CV). Also, good recoveries were obtained with both synthetic mixtures and commercial tablets. The proposed method was successfully applied for simultaneous determination of simvastatin and ezetimibe in a pharmaceutical dosage form without any interference from excipients.http://dx.doi.org/10.1155/2010/423057 |
| spellingShingle | Effat Souri Masoud Amanlou Development and Validation of a Derivative Spectrophotometric Method for Simultaneous Determination of Simvastatin and Ezetimibe E-Journal of Chemistry |
| title | Development and Validation of a Derivative Spectrophotometric Method for Simultaneous Determination of Simvastatin and Ezetimibe |
| title_full | Development and Validation of a Derivative Spectrophotometric Method for Simultaneous Determination of Simvastatin and Ezetimibe |
| title_fullStr | Development and Validation of a Derivative Spectrophotometric Method for Simultaneous Determination of Simvastatin and Ezetimibe |
| title_full_unstemmed | Development and Validation of a Derivative Spectrophotometric Method for Simultaneous Determination of Simvastatin and Ezetimibe |
| title_short | Development and Validation of a Derivative Spectrophotometric Method for Simultaneous Determination of Simvastatin and Ezetimibe |
| title_sort | development and validation of a derivative spectrophotometric method for simultaneous determination of simvastatin and ezetimibe |
| url | http://dx.doi.org/10.1155/2010/423057 |
| work_keys_str_mv | AT effatsouri developmentandvalidationofaderivativespectrophotometricmethodforsimultaneousdeterminationofsimvastatinandezetimibe AT masoudamanlou developmentandvalidationofaderivativespectrophotometricmethodforsimultaneousdeterminationofsimvastatinandezetimibe |