Extension of Chronological Lifespan by Hexokinase Mutation in Kluyveromyces lactis Involves Increased Level of the Mitochondrial Chaperonin Hsp60
Oxidative damage, mitochondrial dysfunction, genomic instability, and telomere shortening represent all molecular processes proposed as causal factors in aging. Lifespan can be increased by metabolism through an influence on such processes. Glucose reduction extends chronological lifespan (CLS) of S...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Aging Research |
| Online Access: | http://dx.doi.org/10.1155/2012/946586 |
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| author | Lisa Rizzetto Elena Zanni Daniela Uccelletti Ileana Ferrero Paola Goffrini |
| author_facet | Lisa Rizzetto Elena Zanni Daniela Uccelletti Ileana Ferrero Paola Goffrini |
| author_sort | Lisa Rizzetto |
| collection | DOAJ |
| description | Oxidative damage, mitochondrial dysfunction, genomic instability, and telomere shortening represent all molecular processes proposed as causal factors in aging. Lifespan can be increased by metabolism through an influence on such processes. Glucose reduction extends chronological lifespan (CLS) of Saccharomyces cerevisiae through metabolic adaptation to respiration. To answer the question if the reduced CLS could be ascribed to glucose per se or to glucose repression of respiratory enzymes, we used the Kluyveromyces lactis yeast, where glucose repression does not affect the respiratory function. We identified the unique hexokinase, encoded by RAG5 gene, as an important player in influencing yeast lifespan by modulating mitochondrial functionality and the level of the mitochondrial chaperonin Hsp60. In this context, this hexokinase might have a regulatory role in the influence of CLS, shedding new light on the complex regulation played by hexokinases. |
| format | Article |
| id | doaj-art-d7d0733eeeaf448dbca9e1f9b8f80b32 |
| institution | Kabale University |
| issn | 2090-2204 2090-2212 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Aging Research |
| spelling | doaj-art-d7d0733eeeaf448dbca9e1f9b8f80b322025-02-03T01:25:27ZengWileyJournal of Aging Research2090-22042090-22122012-01-01201210.1155/2012/946586946586Extension of Chronological Lifespan by Hexokinase Mutation in Kluyveromyces lactis Involves Increased Level of the Mitochondrial Chaperonin Hsp60Lisa Rizzetto0Elena Zanni1Daniela Uccelletti2Ileana Ferrero3Paola Goffrini4Department of Genetics, University of Parma, Parco Area delle Scienze 11/A, 43100 Parma, ItalyDepartment of Biology and Biotechnology “Charles Darwin”, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, ItalyDepartment of Biology and Biotechnology “Charles Darwin”, Sapienza University of Rome, P.le A. Moro 5, 00185 Rome, ItalyDepartment of Genetics, University of Parma, Parco Area delle Scienze 11/A, 43100 Parma, ItalyDepartment of Genetics, University of Parma, Parco Area delle Scienze 11/A, 43100 Parma, ItalyOxidative damage, mitochondrial dysfunction, genomic instability, and telomere shortening represent all molecular processes proposed as causal factors in aging. Lifespan can be increased by metabolism through an influence on such processes. Glucose reduction extends chronological lifespan (CLS) of Saccharomyces cerevisiae through metabolic adaptation to respiration. To answer the question if the reduced CLS could be ascribed to glucose per se or to glucose repression of respiratory enzymes, we used the Kluyveromyces lactis yeast, where glucose repression does not affect the respiratory function. We identified the unique hexokinase, encoded by RAG5 gene, as an important player in influencing yeast lifespan by modulating mitochondrial functionality and the level of the mitochondrial chaperonin Hsp60. In this context, this hexokinase might have a regulatory role in the influence of CLS, shedding new light on the complex regulation played by hexokinases.http://dx.doi.org/10.1155/2012/946586 |
| spellingShingle | Lisa Rizzetto Elena Zanni Daniela Uccelletti Ileana Ferrero Paola Goffrini Extension of Chronological Lifespan by Hexokinase Mutation in Kluyveromyces lactis Involves Increased Level of the Mitochondrial Chaperonin Hsp60 Journal of Aging Research |
| title | Extension of Chronological Lifespan by Hexokinase Mutation in Kluyveromyces lactis Involves Increased Level of the Mitochondrial Chaperonin Hsp60 |
| title_full | Extension of Chronological Lifespan by Hexokinase Mutation in Kluyveromyces lactis Involves Increased Level of the Mitochondrial Chaperonin Hsp60 |
| title_fullStr | Extension of Chronological Lifespan by Hexokinase Mutation in Kluyveromyces lactis Involves Increased Level of the Mitochondrial Chaperonin Hsp60 |
| title_full_unstemmed | Extension of Chronological Lifespan by Hexokinase Mutation in Kluyveromyces lactis Involves Increased Level of the Mitochondrial Chaperonin Hsp60 |
| title_short | Extension of Chronological Lifespan by Hexokinase Mutation in Kluyveromyces lactis Involves Increased Level of the Mitochondrial Chaperonin Hsp60 |
| title_sort | extension of chronological lifespan by hexokinase mutation in kluyveromyces lactis involves increased level of the mitochondrial chaperonin hsp60 |
| url | http://dx.doi.org/10.1155/2012/946586 |
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