Comparative analysis of AAV serotypes for transduction of olfactory sensory neurons
Olfactory sensory neurons within the nasal epithelium detect volatile odorants and relay odor information to the central nervous system. Unlike other sensory inputs, olfactory sensory neurons interface with the external environment and project their axons directly into the central nervous system. Th...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Neuroscience |
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| author | Benjamin D. W. Belfort Benjamin D. W. Belfort Benjamin D. W. Belfort Benjamin D. W. Belfort Benjamin D. W. Belfort Johnathan D. Jia Johnathan D. Jia Johnathan D. Jia Alexandra R. Garza Alexandra R. Garza Alexandra R. Garza Alexandra R. Garza Anthony M. Insalaco Anthony M. Insalaco J. P. McGinnis J. P. McGinnis Brandon T. Pekarek Brandon T. Pekarek Brandon T. Pekarek Joshua Ortiz-Guzman Burak Tepe Burak Tepe Hu Chen Hu Chen Hu Chen aSCENT-PD Investigators Zhandong Liu Zhandong Liu Zhandong Liu Benjamin R. Arenkiel Benjamin R. Arenkiel Benjamin R. Arenkiel Benjamin R. Arenkiel Benjamin Arenkiel Zhandong Liu Brit Mollenhauer Josef Penninger Maxime Rousseaux Armen Saghatelyan Natalina Salmaso Christine Stadelmann Michael G. Schlossmacher Julianna J. Tomlinson John M. Woulfe Ying Wang Zihong Chen Mira Jeong |
| author_facet | Benjamin D. W. Belfort Benjamin D. W. Belfort Benjamin D. W. Belfort Benjamin D. W. Belfort Benjamin D. W. Belfort Johnathan D. Jia Johnathan D. Jia Johnathan D. Jia Alexandra R. Garza Alexandra R. Garza Alexandra R. Garza Alexandra R. Garza Anthony M. Insalaco Anthony M. Insalaco J. P. McGinnis J. P. McGinnis Brandon T. Pekarek Brandon T. Pekarek Brandon T. Pekarek Joshua Ortiz-Guzman Burak Tepe Burak Tepe Hu Chen Hu Chen Hu Chen aSCENT-PD Investigators Zhandong Liu Zhandong Liu Zhandong Liu Benjamin R. Arenkiel Benjamin R. Arenkiel Benjamin R. Arenkiel Benjamin R. Arenkiel Benjamin Arenkiel Zhandong Liu Brit Mollenhauer Josef Penninger Maxime Rousseaux Armen Saghatelyan Natalina Salmaso Christine Stadelmann Michael G. Schlossmacher Julianna J. Tomlinson John M. Woulfe Ying Wang Zihong Chen Mira Jeong |
| author_sort | Benjamin D. W. Belfort |
| collection | DOAJ |
| description | Olfactory sensory neurons within the nasal epithelium detect volatile odorants and relay odor information to the central nervous system. Unlike other sensory inputs, olfactory sensory neurons interface with the external environment and project their axons directly into the central nervous system. The use of adeno-associated viruses to target these neurons has garnered interest for applications in gene therapy, probing olfactory sensory neuron biology, and modeling disease. To date, there is no consensus on the optimal AAV serotype for efficient and selective transduction of olfactory sensory neurons in vivo. Here we utilized serial confocal imaging and single-nucleus RNA sequencing to evaluate the efficacy of 11 different AAV serotypes in transducing murine olfactory sensory neurons via non-invasive nasal inoculation. Our results reveal that AAV1, while highly effective, exhibited broad tropism, whereas AAV-DJ/8 showed the greatest specificity for olfactory sensory neurons. |
| format | Article |
| id | doaj-art-d7b642777bcc4587b81b8a3d8e3a95eb |
| institution | DOAJ |
| issn | 1662-453X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj-art-d7b642777bcc4587b81b8a3d8e3a95eb2025-08-20T03:11:33ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2025-02-011910.3389/fnins.2025.15311221531122Comparative analysis of AAV serotypes for transduction of olfactory sensory neuronsBenjamin D. W. Belfort0Benjamin D. W. Belfort1Benjamin D. W. Belfort2Benjamin D. W. Belfort3Benjamin D. W. Belfort4Johnathan D. Jia5Johnathan D. Jia6Johnathan D. Jia7Alexandra R. Garza8Alexandra R. Garza9Alexandra R. Garza10Alexandra R. Garza11Anthony M. Insalaco12Anthony M. Insalaco13J. P. McGinnis14J. P. McGinnis15Brandon T. Pekarek16Brandon T. Pekarek17Brandon T. Pekarek18Joshua Ortiz-Guzman19Burak Tepe20Burak Tepe21Hu Chen22Hu Chen23Hu Chen24aSCENT-PD Investigators25Zhandong Liu26Zhandong Liu27Zhandong Liu28Benjamin R. Arenkiel29Benjamin R. Arenkiel30Benjamin R. Arenkiel31Benjamin R. Arenkiel32Benjamin ArenkielZhandong LiuBrit MollenhauerJosef PenningerMaxime RousseauxArmen SaghatelyanNatalina SalmasoChristine StadelmannMichael G. SchlossmacherJulianna J. TomlinsonJohn M. WoulfeYing WangZihong ChenMira JeongGenetics and Genomics Graduate Program, Baylor College of Medicine, Houston, TX, United StatesDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, United StatesJan and Dan Duncan Neurological Research Institute, Texas Children’s Hospital, Houston, TX, United StatesMedical Scientist Training Program, Baylor College of Medicine, Houston, TX, United StatesAligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD, United StatesJan and Dan Duncan Neurological Research Institute, Texas Children’s Hospital, Houston, TX, United StatesAligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD, United StatesSection of Neurology, Department of Pediatrics, Baylor College of Medicine, Houston, TX, United StatesGenetics and Genomics Graduate Program, Baylor College of Medicine, Houston, TX, United StatesDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, United StatesJan and Dan Duncan Neurological Research Institute, Texas Children’s Hospital, Houston, TX, United StatesAligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD, United StatesDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, United StatesJan and Dan Duncan Neurological Research Institute, Texas Children’s Hospital, Houston, TX, United StatesJan and Dan Duncan Neurological Research Institute, Texas Children’s Hospital, Houston, TX, United StatesDepartment of Neurosurgery, Baylor College of Medicine, Houston, TX, United StatesGenetics and Genomics Graduate Program, Baylor College of Medicine, Houston, TX, United StatesDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, United StatesJan and Dan Duncan Neurological Research Institute, Texas Children’s Hospital, Houston, TX, United StatesJan and Dan Duncan Neurological Research Institute, Texas Children’s Hospital, Houston, TX, United StatesDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, United StatesJan and Dan Duncan Neurological Research Institute, Texas Children’s Hospital, Houston, TX, United StatesJan and Dan Duncan Neurological Research Institute, Texas Children’s Hospital, Houston, TX, United StatesAligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD, United StatesSection of Neurology, Department of Pediatrics, Baylor College of Medicine, Houston, TX, United StatesAligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD, United StatesJan and Dan Duncan Neurological Research Institute, Texas Children’s Hospital, Houston, TX, United StatesAligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD, United StatesSection of Neurology, Department of Pediatrics, Baylor College of Medicine, Houston, TX, United StatesDepartment of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, United StatesJan and Dan Duncan Neurological Research Institute, Texas Children’s Hospital, Houston, TX, United StatesAligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD, United StatesDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, United StatesOlfactory sensory neurons within the nasal epithelium detect volatile odorants and relay odor information to the central nervous system. Unlike other sensory inputs, olfactory sensory neurons interface with the external environment and project their axons directly into the central nervous system. The use of adeno-associated viruses to target these neurons has garnered interest for applications in gene therapy, probing olfactory sensory neuron biology, and modeling disease. To date, there is no consensus on the optimal AAV serotype for efficient and selective transduction of olfactory sensory neurons in vivo. Here we utilized serial confocal imaging and single-nucleus RNA sequencing to evaluate the efficacy of 11 different AAV serotypes in transducing murine olfactory sensory neurons via non-invasive nasal inoculation. Our results reveal that AAV1, while highly effective, exhibited broad tropism, whereas AAV-DJ/8 showed the greatest specificity for olfactory sensory neurons.https://www.frontiersin.org/articles/10.3389/fnins.2025.1531122/fullAAVsnRNAseqgene therapyolfactionolfactory sensory neurons |
| spellingShingle | Benjamin D. W. Belfort Benjamin D. W. Belfort Benjamin D. W. Belfort Benjamin D. W. Belfort Benjamin D. W. Belfort Johnathan D. Jia Johnathan D. Jia Johnathan D. Jia Alexandra R. Garza Alexandra R. Garza Alexandra R. Garza Alexandra R. Garza Anthony M. Insalaco Anthony M. Insalaco J. P. McGinnis J. P. McGinnis Brandon T. Pekarek Brandon T. Pekarek Brandon T. Pekarek Joshua Ortiz-Guzman Burak Tepe Burak Tepe Hu Chen Hu Chen Hu Chen aSCENT-PD Investigators Zhandong Liu Zhandong Liu Zhandong Liu Benjamin R. Arenkiel Benjamin R. Arenkiel Benjamin R. Arenkiel Benjamin R. Arenkiel Benjamin Arenkiel Zhandong Liu Brit Mollenhauer Josef Penninger Maxime Rousseaux Armen Saghatelyan Natalina Salmaso Christine Stadelmann Michael G. Schlossmacher Julianna J. Tomlinson John M. Woulfe Ying Wang Zihong Chen Mira Jeong Comparative analysis of AAV serotypes for transduction of olfactory sensory neurons Frontiers in Neuroscience AAV snRNAseq gene therapy olfaction olfactory sensory neurons |
| title | Comparative analysis of AAV serotypes for transduction of olfactory sensory neurons |
| title_full | Comparative analysis of AAV serotypes for transduction of olfactory sensory neurons |
| title_fullStr | Comparative analysis of AAV serotypes for transduction of olfactory sensory neurons |
| title_full_unstemmed | Comparative analysis of AAV serotypes for transduction of olfactory sensory neurons |
| title_short | Comparative analysis of AAV serotypes for transduction of olfactory sensory neurons |
| title_sort | comparative analysis of aav serotypes for transduction of olfactory sensory neurons |
| topic | AAV snRNAseq gene therapy olfaction olfactory sensory neurons |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2025.1531122/full |
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