Radiotherapy Suppresses Bone Cancer Pain through Inhibiting Activation of cAMP Signaling in Rat Dorsal Root Ganglion and Spinal Cord
Radiotherapy is one of the major clinical approaches for treatment of bone cancer pain. Activation of cAMP-PKA signaling pathway plays important roles in bone cancer pain. Here, we examined the effects of radiotherapy on bone cancer pain and accompanying abnormal activation of cAMP-PKA signaling. Fe...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/5093095 |
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| author | Guiqin Zhu Yanbin Dong Xueming He Ping Zhao Aixing Yang Rubing Zhou Jianhua Ma Zhong Xie Xue-Jun Song |
| author_facet | Guiqin Zhu Yanbin Dong Xueming He Ping Zhao Aixing Yang Rubing Zhou Jianhua Ma Zhong Xie Xue-Jun Song |
| author_sort | Guiqin Zhu |
| collection | DOAJ |
| description | Radiotherapy is one of the major clinical approaches for treatment of bone cancer pain. Activation of cAMP-PKA signaling pathway plays important roles in bone cancer pain. Here, we examined the effects of radiotherapy on bone cancer pain and accompanying abnormal activation of cAMP-PKA signaling. Female Sprague-Dawley rats were used and received tumor cell implantation (TCI) in rat tibia (TCI cancer pain model). Some of the rats that previously received TCI treatment were treated with X-ray radiation (radiotherapy). Thermal hyperalgesia and mechanical allodynia were measured and used for evaluating level of pain caused by TCI treatment. PKA mRNA expression in dorsal root ganglion (DRG) was detected by RT-PCR. Concentrations of cAMP, IL-1β, and TNF-α as well as PKA activity in DRG and the spinal cord were measured by ELISA. The results showed that radiotherapy significantly suppressed TCI-induced thermal hyperalgesia and mechanical allodynia. The level of PKA mRNA in DRG, cAMP concentration and PKA activity in DRG and in the spinal cord, and concentrations of IL-1β and TNF-α in the spinal cord were significantly reduced by radiotherapy. In addition, radiotherapy also reduced TCI-induced bone loss. These findings suggest that radiotherapy may suppress bone cancer pain through inhibition of activation of cAMP-PKA signaling pathway in DRG and the spinal cord. |
| format | Article |
| id | doaj-art-d7acd8552ac049c3991ef8a7bec921f8 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-d7acd8552ac049c3991ef8a7bec921f82025-02-03T01:11:45ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/50930955093095Radiotherapy Suppresses Bone Cancer Pain through Inhibiting Activation of cAMP Signaling in Rat Dorsal Root Ganglion and Spinal CordGuiqin Zhu0Yanbin Dong1Xueming He2Ping Zhao3Aixing Yang4Rubing Zhou5Jianhua Ma6Zhong Xie7Xue-Jun Song8Center for Clinical Research and Translational Medicine, Lianyungang Oriental Hospital, Lianyungang, Jiangsu 222042, ChinaCenter for Clinical Research and Translational Medicine, Lianyungang Oriental Hospital, Lianyungang, Jiangsu 222042, ChinaCenter for Clinical Research and Translational Medicine, Lianyungang Oriental Hospital, Lianyungang, Jiangsu 222042, ChinaCenter for Clinical Research and Translational Medicine, Lianyungang Oriental Hospital, Lianyungang, Jiangsu 222042, ChinaCenter for Clinical Research and Translational Medicine, Lianyungang Oriental Hospital, Lianyungang, Jiangsu 222042, ChinaCenter for Clinical Research and Translational Medicine, Lianyungang Oriental Hospital, Lianyungang, Jiangsu 222042, ChinaCenter for Clinical Research and Translational Medicine, Lianyungang Oriental Hospital, Lianyungang, Jiangsu 222042, ChinaCenter for Clinical Research and Translational Medicine, Lianyungang Oriental Hospital, Lianyungang, Jiangsu 222042, ChinaCenter for Clinical Research and Translational Medicine, Lianyungang Oriental Hospital, Lianyungang, Jiangsu 222042, ChinaRadiotherapy is one of the major clinical approaches for treatment of bone cancer pain. Activation of cAMP-PKA signaling pathway plays important roles in bone cancer pain. Here, we examined the effects of radiotherapy on bone cancer pain and accompanying abnormal activation of cAMP-PKA signaling. Female Sprague-Dawley rats were used and received tumor cell implantation (TCI) in rat tibia (TCI cancer pain model). Some of the rats that previously received TCI treatment were treated with X-ray radiation (radiotherapy). Thermal hyperalgesia and mechanical allodynia were measured and used for evaluating level of pain caused by TCI treatment. PKA mRNA expression in dorsal root ganglion (DRG) was detected by RT-PCR. Concentrations of cAMP, IL-1β, and TNF-α as well as PKA activity in DRG and the spinal cord were measured by ELISA. The results showed that radiotherapy significantly suppressed TCI-induced thermal hyperalgesia and mechanical allodynia. The level of PKA mRNA in DRG, cAMP concentration and PKA activity in DRG and in the spinal cord, and concentrations of IL-1β and TNF-α in the spinal cord were significantly reduced by radiotherapy. In addition, radiotherapy also reduced TCI-induced bone loss. These findings suggest that radiotherapy may suppress bone cancer pain through inhibition of activation of cAMP-PKA signaling pathway in DRG and the spinal cord.http://dx.doi.org/10.1155/2016/5093095 |
| spellingShingle | Guiqin Zhu Yanbin Dong Xueming He Ping Zhao Aixing Yang Rubing Zhou Jianhua Ma Zhong Xie Xue-Jun Song Radiotherapy Suppresses Bone Cancer Pain through Inhibiting Activation of cAMP Signaling in Rat Dorsal Root Ganglion and Spinal Cord Mediators of Inflammation |
| title | Radiotherapy Suppresses Bone Cancer Pain through Inhibiting Activation of cAMP Signaling in Rat Dorsal Root Ganglion and Spinal Cord |
| title_full | Radiotherapy Suppresses Bone Cancer Pain through Inhibiting Activation of cAMP Signaling in Rat Dorsal Root Ganglion and Spinal Cord |
| title_fullStr | Radiotherapy Suppresses Bone Cancer Pain through Inhibiting Activation of cAMP Signaling in Rat Dorsal Root Ganglion and Spinal Cord |
| title_full_unstemmed | Radiotherapy Suppresses Bone Cancer Pain through Inhibiting Activation of cAMP Signaling in Rat Dorsal Root Ganglion and Spinal Cord |
| title_short | Radiotherapy Suppresses Bone Cancer Pain through Inhibiting Activation of cAMP Signaling in Rat Dorsal Root Ganglion and Spinal Cord |
| title_sort | radiotherapy suppresses bone cancer pain through inhibiting activation of camp signaling in rat dorsal root ganglion and spinal cord |
| url | http://dx.doi.org/10.1155/2016/5093095 |
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