Analysis of the ASMT Gene Family in Pepper (Capsicum annuum L.): Identification, Phylogeny, and Expression Profiles
Acetylserotonin methyltransferase (ASMT) in plant species, one of the most important enzymes in melatonin biosynthesis, plays a rate-limiting role in the melatonin production. In this study, based on the whole genome sequence, we performed a systematic analysis for the ASMT gene family in pepper (Ca...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2019/7241096 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832561875964919808 |
|---|---|
| author | Luzhao Pan Jiaqiu Zheng Jia Liu Jun Guo Fawan Liu Lecheng Liu Hongjian Wan |
| author_facet | Luzhao Pan Jiaqiu Zheng Jia Liu Jun Guo Fawan Liu Lecheng Liu Hongjian Wan |
| author_sort | Luzhao Pan |
| collection | DOAJ |
| description | Acetylserotonin methyltransferase (ASMT) in plant species, one of the most important enzymes in melatonin biosynthesis, plays a rate-limiting role in the melatonin production. In this study, based on the whole genome sequence, we performed a systematic analysis for the ASMT gene family in pepper (Capsicum annuum L.) and analyzed their expression profiles during growth and development, as well as abiotic stresses. The results showed that at least 16 CaASMT genes were identified in the pepper genome. Phylogenetic analyses of all the CaASMTs were divided into three groups (group I, group II, and group III) with a high bootstrap value. Through the online MEME tool, six distinct motifs (motif 1 to motif 6) were identified. Chromosome location found that most CaASMT genes were mapped in the distal ends of the pepper chromosomes. In addition, RNA-seq analysis revealed that, during the vegetative and reproductive development, the difference in abundance and distinct expression patterns of these CaASMT genes suggests different functions. The qRT-PCR analysis showed that high abundance of CaASMT03, CaASMT04, and CaASMT06 occurred in mature green fruit and mature red fruit. Finally, using RNA-seq and qRT-PCR technology, we also found that several CaASMT genes were induced under abiotic stress conditions. The results will not only contribute to elucidate the evolutionary relationship of ASMT genes but also ascertain the biological function in pepper plant response to abiotic stresses. |
| format | Article |
| id | doaj-art-d7a3b677ad224e39a836d8bfc2eb7e93 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-d7a3b677ad224e39a836d8bfc2eb7e932025-02-03T01:24:03ZengWileyInternational Journal of Genomics2314-436X2314-43782019-01-01201910.1155/2019/72410967241096Analysis of the ASMT Gene Family in Pepper (Capsicum annuum L.): Identification, Phylogeny, and Expression ProfilesLuzhao Pan0Jiaqiu Zheng1Jia Liu2Jun Guo3Fawan Liu4Lecheng Liu5Hongjian Wan6College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, ChinaJiangsu Coastal Area Institute of Agricultural Sciences, Yancheng 224002, ChinaWulanchabu Academy of Agricultural Sciences, Wulanchabu 012000, ChinaJiangsu Coastal Area Institute of Agricultural Sciences, Yancheng 224002, ChinaHorticultural Research Institute, Yunnan Academy of Agricultural Science, Kunming 650231, ChinaCollege of Horticulture and Gardening, Yangtze University, Jingzhou 434025, ChinaState Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaAcetylserotonin methyltransferase (ASMT) in plant species, one of the most important enzymes in melatonin biosynthesis, plays a rate-limiting role in the melatonin production. In this study, based on the whole genome sequence, we performed a systematic analysis for the ASMT gene family in pepper (Capsicum annuum L.) and analyzed their expression profiles during growth and development, as well as abiotic stresses. The results showed that at least 16 CaASMT genes were identified in the pepper genome. Phylogenetic analyses of all the CaASMTs were divided into three groups (group I, group II, and group III) with a high bootstrap value. Through the online MEME tool, six distinct motifs (motif 1 to motif 6) were identified. Chromosome location found that most CaASMT genes were mapped in the distal ends of the pepper chromosomes. In addition, RNA-seq analysis revealed that, during the vegetative and reproductive development, the difference in abundance and distinct expression patterns of these CaASMT genes suggests different functions. The qRT-PCR analysis showed that high abundance of CaASMT03, CaASMT04, and CaASMT06 occurred in mature green fruit and mature red fruit. Finally, using RNA-seq and qRT-PCR technology, we also found that several CaASMT genes were induced under abiotic stress conditions. The results will not only contribute to elucidate the evolutionary relationship of ASMT genes but also ascertain the biological function in pepper plant response to abiotic stresses.http://dx.doi.org/10.1155/2019/7241096 |
| spellingShingle | Luzhao Pan Jiaqiu Zheng Jia Liu Jun Guo Fawan Liu Lecheng Liu Hongjian Wan Analysis of the ASMT Gene Family in Pepper (Capsicum annuum L.): Identification, Phylogeny, and Expression Profiles International Journal of Genomics |
| title | Analysis of the ASMT Gene Family in Pepper (Capsicum annuum L.): Identification, Phylogeny, and Expression Profiles |
| title_full | Analysis of the ASMT Gene Family in Pepper (Capsicum annuum L.): Identification, Phylogeny, and Expression Profiles |
| title_fullStr | Analysis of the ASMT Gene Family in Pepper (Capsicum annuum L.): Identification, Phylogeny, and Expression Profiles |
| title_full_unstemmed | Analysis of the ASMT Gene Family in Pepper (Capsicum annuum L.): Identification, Phylogeny, and Expression Profiles |
| title_short | Analysis of the ASMT Gene Family in Pepper (Capsicum annuum L.): Identification, Phylogeny, and Expression Profiles |
| title_sort | analysis of the asmt gene family in pepper capsicum annuum l identification phylogeny and expression profiles |
| url | http://dx.doi.org/10.1155/2019/7241096 |
| work_keys_str_mv | AT luzhaopan analysisoftheasmtgenefamilyinpeppercapsicumannuumlidentificationphylogenyandexpressionprofiles AT jiaqiuzheng analysisoftheasmtgenefamilyinpeppercapsicumannuumlidentificationphylogenyandexpressionprofiles AT jialiu analysisoftheasmtgenefamilyinpeppercapsicumannuumlidentificationphylogenyandexpressionprofiles AT junguo analysisoftheasmtgenefamilyinpeppercapsicumannuumlidentificationphylogenyandexpressionprofiles AT fawanliu analysisoftheasmtgenefamilyinpeppercapsicumannuumlidentificationphylogenyandexpressionprofiles AT lechengliu analysisoftheasmtgenefamilyinpeppercapsicumannuumlidentificationphylogenyandexpressionprofiles AT hongjianwan analysisoftheasmtgenefamilyinpeppercapsicumannuumlidentificationphylogenyandexpressionprofiles |