In vitro assessment of the nematicidal potential of Streptomyces violascens strain AS2 against root-knot nematodes (Meloidogyne sp.)
Root-knot nematodes (RKNs, Meloidogyne spp.) present a major challenge to sustainable production. The detrimental environmental impacts and increasing resistance associated with synthetic nematicides have increased the need for biocontrol alternatives. Here, we report for the first time the nematici...
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University of Belgrade, University of Novi Sad
2024-01-01
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| Series: | Archives of Biological Sciences |
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| Online Access: | https://doiserbia.nb.rs/img/doi/0354-4664/2024/0354-46642400026A.pdf |
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| author | Azlay Latifa Oubassou Ez-Zahra Berr Alexandre Mayad El Hassan Barakate Mustapha |
| author_facet | Azlay Latifa Oubassou Ez-Zahra Berr Alexandre Mayad El Hassan Barakate Mustapha |
| author_sort | Azlay Latifa |
| collection | DOAJ |
| description | Root-knot nematodes (RKNs, Meloidogyne spp.) present a major challenge to sustainable production. The detrimental environmental impacts and increasing resistance associated with synthetic nematicides have increased the need for biocontrol alternatives. Here, we report for the first time the nematicidal properties of Streptomyces violascens and its viability as a safe and effective solution against nematode infestation. In vitro assays showed that aqueous and ethyl acetate extracts of S. violascens significantly impeded Meloidogyne sp’s reproductive and locomotive functions. The aqueous extract displayed remarkable efficacy, completely inhibiting egg hatching at a concentration of 600 μg/mL, with an LC50 of 168.38 μg/mL, and causing 99% immobility of second-stage juveniles (J2s) at 800 μg/mL within 72 h. Conversely, the organic extract exhibited a 29.3% hatching rate (LC50 of 849.26 μg/mL) and 79% immobility of J2s under comparable conditions. These findings point to the robust nematicidal potential of S. violascens extracts. Enzymatic profiles supported these results, with the selected isolate producing chitinase and protease enzymes capable of degrading nematode eggshells. Our results show that the S. violascens strain can be integrated into a sustainable nematode management practice as an important agent of RKN biocontrol. |
| format | Article |
| id | doaj-art-d79dcbd8792d4e5394259839850673f6 |
| institution | Kabale University |
| issn | 0354-4664 1821-4339 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
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| series | Archives of Biological Sciences |
| spelling | doaj-art-d79dcbd8792d4e5394259839850673f62025-01-30T06:38:33ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392024-01-0176334535810.2298/ABS240711026A0354-46642400026AIn vitro assessment of the nematicidal potential of Streptomyces violascens strain AS2 against root-knot nematodes (Meloidogyne sp.)Azlay Latifa0Oubassou Ez-Zahra1Berr Alexandre2Mayad El Hassan3Barakate Mustapha4Laboratory of Microbial Biotechnology, Agrosciences and, Environment, CNRST Labeled Research Unit No.4, Department of Biology, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco + Laboratory of Biotechnologies and Valorization of Natural Resources, Department of Biology, Faculty of Sciences Agadir, Ibn Zohr University, Agadir, MoroccoLaboratory of Microbial Biotechnology, Agrosciences and, Environment, CNRST Labeled Research Unit No.4, Department of Biology, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco + Institut de Biologie Moléculaire des Plantes (IBMP), CNRS, Université de Strasbourg, , rue du Général Zimmer, Strasbourg, FranceInstitut de Biologie Moléculaire des Plantes (IBMP), CNRS, Université de Strasbourg, Strasbourg, FranceLaboratory of Biotechnologies and Valorization of Natural Resources, Department of Biology, Faculty of Sciences Agadir, Ibn Zohr University, Agadir, MoroccoLaboratory of Microbial Biotechnology, Agrosciences and, Environment, CNRST Labeled Research Unit No.4, Department of Biology, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco + Biodiversity and Plant Sciences Program, AgroBioScience Department, College of Agriculture and Environmental Sciences, Mohammed VI Polytechnic University (UMP), Benguerir, MoroccoRoot-knot nematodes (RKNs, Meloidogyne spp.) present a major challenge to sustainable production. The detrimental environmental impacts and increasing resistance associated with synthetic nematicides have increased the need for biocontrol alternatives. Here, we report for the first time the nematicidal properties of Streptomyces violascens and its viability as a safe and effective solution against nematode infestation. In vitro assays showed that aqueous and ethyl acetate extracts of S. violascens significantly impeded Meloidogyne sp’s reproductive and locomotive functions. The aqueous extract displayed remarkable efficacy, completely inhibiting egg hatching at a concentration of 600 μg/mL, with an LC50 of 168.38 μg/mL, and causing 99% immobility of second-stage juveniles (J2s) at 800 μg/mL within 72 h. Conversely, the organic extract exhibited a 29.3% hatching rate (LC50 of 849.26 μg/mL) and 79% immobility of J2s under comparable conditions. These findings point to the robust nematicidal potential of S. violascens extracts. Enzymatic profiles supported these results, with the selected isolate producing chitinase and protease enzymes capable of degrading nematode eggshells. Our results show that the S. violascens strain can be integrated into a sustainable nematode management practice as an important agent of RKN biocontrol.https://doiserbia.nb.rs/img/doi/0354-4664/2024/0354-46642400026A.pdfactinomycetotameloidogyne sp.nematocidal activityegg hatch inhibitionsecond-stage juvenile mortality |
| spellingShingle | Azlay Latifa Oubassou Ez-Zahra Berr Alexandre Mayad El Hassan Barakate Mustapha In vitro assessment of the nematicidal potential of Streptomyces violascens strain AS2 against root-knot nematodes (Meloidogyne sp.) Archives of Biological Sciences actinomycetota meloidogyne sp. nematocidal activity egg hatch inhibition second-stage juvenile mortality |
| title | In vitro assessment of the nematicidal potential of Streptomyces violascens strain AS2 against root-knot nematodes (Meloidogyne sp.) |
| title_full | In vitro assessment of the nematicidal potential of Streptomyces violascens strain AS2 against root-knot nematodes (Meloidogyne sp.) |
| title_fullStr | In vitro assessment of the nematicidal potential of Streptomyces violascens strain AS2 against root-knot nematodes (Meloidogyne sp.) |
| title_full_unstemmed | In vitro assessment of the nematicidal potential of Streptomyces violascens strain AS2 against root-knot nematodes (Meloidogyne sp.) |
| title_short | In vitro assessment of the nematicidal potential of Streptomyces violascens strain AS2 against root-knot nematodes (Meloidogyne sp.) |
| title_sort | in vitro assessment of the nematicidal potential of streptomyces violascens strain as2 against root knot nematodes meloidogyne sp |
| topic | actinomycetota meloidogyne sp. nematocidal activity egg hatch inhibition second-stage juvenile mortality |
| url | https://doiserbia.nb.rs/img/doi/0354-4664/2024/0354-46642400026A.pdf |
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