The Assessment of Cholinesterase from the Liver of Puntius Javanicus as Detection of Metal Ions
Crude extract of ChE from the liver of Puntius javanicus was purified using procainamide-sepharyl 6B. S-Butyrylthiocholine iodide (BTC) was selected as the specific synthetic substrate for this assay with the highest maximal velocity and lowest biomolecular constant at 53.49 µmole/min/mg and 0.23 mM...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/571094 |
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| author | Mohd Khalizan Sabullah Mohd Rosni Sulaiman Mohd Yunus Abd Shukor Mohd Arif Syed Nor Aripin Shamaan Ariff Khalid Siti Aqlima Ahmad |
| author_facet | Mohd Khalizan Sabullah Mohd Rosni Sulaiman Mohd Yunus Abd Shukor Mohd Arif Syed Nor Aripin Shamaan Ariff Khalid Siti Aqlima Ahmad |
| author_sort | Mohd Khalizan Sabullah |
| collection | DOAJ |
| description | Crude extract of ChE from the liver of Puntius javanicus was purified using procainamide-sepharyl 6B. S-Butyrylthiocholine iodide (BTC) was selected as the specific synthetic substrate for this assay with the highest maximal velocity and lowest biomolecular constant at 53.49 µmole/min/mg and 0.23 mM, respectively, with catalytic efficiency ratio of 0.23. The optimum parameter was obtained at pH 7.5 and optimal temperature in the range of 25 to 30°C. The effect of different storage condition was assessed where ChE activity was significantly decreased after 9 days of storage at room temperature. However, ChE activity showed no significant difference when stored at 4.0, 0, and −25°C for 15 days. Screening of heavy metals shows that chromium, copper, and mercury strongly inhibited P. javanicus ChE by lowering the activity below 50%, while several pairwise combination of metal ions exhibited synergistic inhibiting effects on the enzyme which is greater than single exposure especially chromium, copper, and mercury. The results showed that P. javanicus ChE has the potential to be used as a biosensor for the detection of metal ions. |
| format | Article |
| id | doaj-art-d79d15f72d3b4add9d1ce8bb213416f6 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-d79d15f72d3b4add9d1ce8bb213416f62025-02-03T01:25:32ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/571094571094The Assessment of Cholinesterase from the Liver of Puntius Javanicus as Detection of Metal IonsMohd Khalizan Sabullah0Mohd Rosni Sulaiman1Mohd Yunus Abd Shukor2Mohd Arif Syed3Nor Aripin Shamaan4Ariff Khalid5Siti Aqlima Ahmad6Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, MalaysiaFaculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, MalaysiaDepartment of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, MalaysiaDepartment of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, MalaysiaFaculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia, 13th Floor, Menara B, Persiaran MPAJ, Jalan Pandan Utama, Pandan Indah, 55100 Kuala Lumpur, MalaysiaBiomedical Science Program, Faculty of Biomedicine and Health, Asia Metropolitan University, 43200 Cheras, Selangor, MalaysiaDepartment of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, MalaysiaCrude extract of ChE from the liver of Puntius javanicus was purified using procainamide-sepharyl 6B. S-Butyrylthiocholine iodide (BTC) was selected as the specific synthetic substrate for this assay with the highest maximal velocity and lowest biomolecular constant at 53.49 µmole/min/mg and 0.23 mM, respectively, with catalytic efficiency ratio of 0.23. The optimum parameter was obtained at pH 7.5 and optimal temperature in the range of 25 to 30°C. The effect of different storage condition was assessed where ChE activity was significantly decreased after 9 days of storage at room temperature. However, ChE activity showed no significant difference when stored at 4.0, 0, and −25°C for 15 days. Screening of heavy metals shows that chromium, copper, and mercury strongly inhibited P. javanicus ChE by lowering the activity below 50%, while several pairwise combination of metal ions exhibited synergistic inhibiting effects on the enzyme which is greater than single exposure especially chromium, copper, and mercury. The results showed that P. javanicus ChE has the potential to be used as a biosensor for the detection of metal ions.http://dx.doi.org/10.1155/2014/571094 |
| spellingShingle | Mohd Khalizan Sabullah Mohd Rosni Sulaiman Mohd Yunus Abd Shukor Mohd Arif Syed Nor Aripin Shamaan Ariff Khalid Siti Aqlima Ahmad The Assessment of Cholinesterase from the Liver of Puntius Javanicus as Detection of Metal Ions The Scientific World Journal |
| title | The Assessment of Cholinesterase from the Liver of Puntius Javanicus as Detection of Metal Ions |
| title_full | The Assessment of Cholinesterase from the Liver of Puntius Javanicus as Detection of Metal Ions |
| title_fullStr | The Assessment of Cholinesterase from the Liver of Puntius Javanicus as Detection of Metal Ions |
| title_full_unstemmed | The Assessment of Cholinesterase from the Liver of Puntius Javanicus as Detection of Metal Ions |
| title_short | The Assessment of Cholinesterase from the Liver of Puntius Javanicus as Detection of Metal Ions |
| title_sort | assessment of cholinesterase from the liver of puntius javanicus as detection of metal ions |
| url | http://dx.doi.org/10.1155/2014/571094 |
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