Effect of fructooligosaccharides on the colonization of Lactobacillus rhamnosus AS 1.2466T in the gut of mice
Lactobacillus rhamnosus and fructooligosaccharides (FOS) have been widely studied so far. However, the effects of L. rhamnosus on the intestinal microecological environment at the species level and the effect of different proportions of FOS on L. rhamnosus colonization in different parts of mice int...
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| Format: | Article |
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Tsinghua University Press
2023-03-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022001756 |
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| author | Zhihua Niu Meijuan Zou Tingting Bei Na Zhang Dongyao Li Miaoshu Wang Chen Li Hongtao Tian |
| author_facet | Zhihua Niu Meijuan Zou Tingting Bei Na Zhang Dongyao Li Miaoshu Wang Chen Li Hongtao Tian |
| author_sort | Zhihua Niu |
| collection | DOAJ |
| description | Lactobacillus rhamnosus and fructooligosaccharides (FOS) have been widely studied so far. However, the effects of L. rhamnosus on the intestinal microecological environment at the species level and the effect of different proportions of FOS on L. rhamnosus colonization in different parts of mice intestine are still unclear. The study results indicated that the specific bands of enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) in the L. rhamnosus (LR) group significantly increased at 7 days. Although the number of bands was similar to the natural recovery (NR) group, the brightness of few bands significantly enhanced in the later stage of recovery. Besides, Southern-blot maps showed strong signals, indicating that the ERIC-PCR fingerprint could accurately reflect the changes in the mouse gut microbiota diversity. Further, the high-throughput results confirmed that the Lactobacillus and Akkermansia had different changes at different periods, but all of them showed an upward trend, while the Klebsiella were inhibited, thereby maintaining the intestinal microecology balance. Moreover, FOS exerted a positive effect on L. rhamnosus colonization in the gut. |
| format | Article |
| id | doaj-art-d77b4fe92a1e45fc80e34120ae176b96 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-d77b4fe92a1e45fc80e34120ae176b962025-02-03T04:06:19ZengTsinghua University PressFood Science and Human Wellness2213-45302023-03-01122607613Effect of fructooligosaccharides on the colonization of Lactobacillus rhamnosus AS 1.2466T in the gut of miceZhihua Niu0Meijuan Zou1Tingting Bei2Na Zhang3Dongyao Li4Miaoshu Wang5Chen Li6Hongtao Tian7College of Food Science and Technology, Hebei Agricultural University, Baoding 071000, ChinaCollege of Food Science and Technology, Hebei Agricultural University, Baoding 071000, ChinaCollege of Food Science and Technology, Hebei Agricultural University, Baoding 071000, ChinaCollege of Food Science and Technology, Hebei Agricultural University, Baoding 071000, China; College of Biochemistry and Environmental Engineering, Baoding University, Baoding 071000, ChinaCollege of Food Science and Technology, Hebei Agricultural University, Baoding 071000, ChinaNew Hope Tensun (Hebei) Dairy Co., Ltd, Baoding 071000, China; Hebei Technology Innovation Center of Probiotic Functional Dairy Product, Baoding 071000, ChinaCollege of Food Science and Technology, Hebei Agricultural University, Baoding 071000, China; Hebei Technology Innovation Center of Probiotic Functional Dairy Product, Baoding 071000, China; Corresponding authors at: College of Food Science and Technology, Hebei Agricultural University, Baoding 071000, ChinaCollege of Food Science and Technology, Hebei Agricultural University, Baoding 071000, China; Hebei Technology Innovation Center of Probiotic Functional Dairy Product, Baoding 071000, China; National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding 071000, China; Corresponding authors at: College of Food Science and Technology, Hebei Agricultural University, Baoding 071000, ChinaLactobacillus rhamnosus and fructooligosaccharides (FOS) have been widely studied so far. However, the effects of L. rhamnosus on the intestinal microecological environment at the species level and the effect of different proportions of FOS on L. rhamnosus colonization in different parts of mice intestine are still unclear. The study results indicated that the specific bands of enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) in the L. rhamnosus (LR) group significantly increased at 7 days. Although the number of bands was similar to the natural recovery (NR) group, the brightness of few bands significantly enhanced in the later stage of recovery. Besides, Southern-blot maps showed strong signals, indicating that the ERIC-PCR fingerprint could accurately reflect the changes in the mouse gut microbiota diversity. Further, the high-throughput results confirmed that the Lactobacillus and Akkermansia had different changes at different periods, but all of them showed an upward trend, while the Klebsiella were inhibited, thereby maintaining the intestinal microecology balance. Moreover, FOS exerted a positive effect on L. rhamnosus colonization in the gut.http://www.sciencedirect.com/science/article/pii/S2213453022001756Intestinal microbiotaSynbioticsFructooligosaccharidesLactobacillus rhamnosus |
| spellingShingle | Zhihua Niu Meijuan Zou Tingting Bei Na Zhang Dongyao Li Miaoshu Wang Chen Li Hongtao Tian Effect of fructooligosaccharides on the colonization of Lactobacillus rhamnosus AS 1.2466T in the gut of mice Food Science and Human Wellness Intestinal microbiota Synbiotics Fructooligosaccharides Lactobacillus rhamnosus |
| title | Effect of fructooligosaccharides on the colonization of Lactobacillus rhamnosus AS 1.2466T in the gut of mice |
| title_full | Effect of fructooligosaccharides on the colonization of Lactobacillus rhamnosus AS 1.2466T in the gut of mice |
| title_fullStr | Effect of fructooligosaccharides on the colonization of Lactobacillus rhamnosus AS 1.2466T in the gut of mice |
| title_full_unstemmed | Effect of fructooligosaccharides on the colonization of Lactobacillus rhamnosus AS 1.2466T in the gut of mice |
| title_short | Effect of fructooligosaccharides on the colonization of Lactobacillus rhamnosus AS 1.2466T in the gut of mice |
| title_sort | effect of fructooligosaccharides on the colonization of lactobacillus rhamnosus as 1 2466t in the gut of mice |
| topic | Intestinal microbiota Synbiotics Fructooligosaccharides Lactobacillus rhamnosus |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022001756 |
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