Prevention of Cyclophosphamide-Induced Immunosuppression in Mice with the Antimicrobial Peptide Sublancin
Sublancin is a glycosylated antimicrobial peptide produced by Bacillus subtilis 168 with combined antibacterial and immunomodulatory activities. The purpose of this study was to evaluate the protective effects of sublancin on immunosuppression in cyclophosphamide-treated mice. In normal mice, the ph...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2018/4353580 |
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| author | Shuai Wang Shuo Huang Qianhong Ye Xiangfang Zeng Haitao Yu Desheng Qi Shiyan Qiao |
| author_facet | Shuai Wang Shuo Huang Qianhong Ye Xiangfang Zeng Haitao Yu Desheng Qi Shiyan Qiao |
| author_sort | Shuai Wang |
| collection | DOAJ |
| description | Sublancin is a glycosylated antimicrobial peptide produced by Bacillus subtilis 168 with combined antibacterial and immunomodulatory activities. The purpose of this study was to evaluate the protective effects of sublancin on immunosuppression in cyclophosphamide-treated mice. In normal mice, the phagocytic activity of mouse peritoneal macrophages was significantly enhanced by oral administration of sublancin (1.0 mg/kg body weight) to BALB/c mice for 7 days (P<0.01). In addition, the mRNA expression of IL-1β, IL-6, and TNF-α in peritoneal macrophages from sublancin- (1.0 mg/kg body weight) administered mice was significantly increased (P<0.05). In cyclophosphamide-treated mice, oral sublancin administration accelerated the recovery of peripheral white blood cells, red blood cells, hemoglobins, and platelets and enhanced the macrophage phagocytic activity. Furthermore, sublancin restored the mRNA levels of IL-2, IL-4, and IL-6 in the spleen. Finally, the intestinal absorption of sublancin was poor as detected in the Caco-2 transwell system. Taken together, these findings suggest that sublancin plays a crucial role in the protection against immunosuppression in cyclophosphamide-treated mice and could be a potential candidate for use in immune therapy regimens. |
| format | Article |
| id | doaj-art-d774ac60b5844174bb56afbb9da227ad |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-d774ac60b5844174bb56afbb9da227ad2025-02-03T01:11:27ZengWileyJournal of Immunology Research2314-88612314-71562018-01-01201810.1155/2018/43535804353580Prevention of Cyclophosphamide-Induced Immunosuppression in Mice with the Antimicrobial Peptide SublancinShuai Wang0Shuo Huang1Qianhong Ye2Xiangfang Zeng3Haitao Yu4Desheng Qi5Shiyan Qiao6State Key Laboratory of Animal Nutrition, Beijing Key Laboratory of Biofeed Additives, Ministry of Agriculture Feed Industry Centre, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Beijing Key Laboratory of Biofeed Additives, Ministry of Agriculture Feed Industry Centre, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Beijing Key Laboratory of Biofeed Additives, Ministry of Agriculture Feed Industry Centre, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Beijing Key Laboratory of Biofeed Additives, Ministry of Agriculture Feed Industry Centre, China Agricultural University, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Beijing Key Laboratory of Biofeed Additives, Ministry of Agriculture Feed Industry Centre, China Agricultural University, Beijing 100193, ChinaDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, ChinaState Key Laboratory of Animal Nutrition, Beijing Key Laboratory of Biofeed Additives, Ministry of Agriculture Feed Industry Centre, China Agricultural University, Beijing 100193, ChinaSublancin is a glycosylated antimicrobial peptide produced by Bacillus subtilis 168 with combined antibacterial and immunomodulatory activities. The purpose of this study was to evaluate the protective effects of sublancin on immunosuppression in cyclophosphamide-treated mice. In normal mice, the phagocytic activity of mouse peritoneal macrophages was significantly enhanced by oral administration of sublancin (1.0 mg/kg body weight) to BALB/c mice for 7 days (P<0.01). In addition, the mRNA expression of IL-1β, IL-6, and TNF-α in peritoneal macrophages from sublancin- (1.0 mg/kg body weight) administered mice was significantly increased (P<0.05). In cyclophosphamide-treated mice, oral sublancin administration accelerated the recovery of peripheral white blood cells, red blood cells, hemoglobins, and platelets and enhanced the macrophage phagocytic activity. Furthermore, sublancin restored the mRNA levels of IL-2, IL-4, and IL-6 in the spleen. Finally, the intestinal absorption of sublancin was poor as detected in the Caco-2 transwell system. Taken together, these findings suggest that sublancin plays a crucial role in the protection against immunosuppression in cyclophosphamide-treated mice and could be a potential candidate for use in immune therapy regimens.http://dx.doi.org/10.1155/2018/4353580 |
| spellingShingle | Shuai Wang Shuo Huang Qianhong Ye Xiangfang Zeng Haitao Yu Desheng Qi Shiyan Qiao Prevention of Cyclophosphamide-Induced Immunosuppression in Mice with the Antimicrobial Peptide Sublancin Journal of Immunology Research |
| title | Prevention of Cyclophosphamide-Induced Immunosuppression in Mice with the Antimicrobial Peptide Sublancin |
| title_full | Prevention of Cyclophosphamide-Induced Immunosuppression in Mice with the Antimicrobial Peptide Sublancin |
| title_fullStr | Prevention of Cyclophosphamide-Induced Immunosuppression in Mice with the Antimicrobial Peptide Sublancin |
| title_full_unstemmed | Prevention of Cyclophosphamide-Induced Immunosuppression in Mice with the Antimicrobial Peptide Sublancin |
| title_short | Prevention of Cyclophosphamide-Induced Immunosuppression in Mice with the Antimicrobial Peptide Sublancin |
| title_sort | prevention of cyclophosphamide induced immunosuppression in mice with the antimicrobial peptide sublancin |
| url | http://dx.doi.org/10.1155/2018/4353580 |
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