An Exploration of the Cellular Microenvironment of the Female Pig Urethra: Translational Insights for Urological Research
Urinary incontinence is a widespread issue, particularly among women, with effective treatments remaining elusive. The pig, and especially the female pig, stands as a promising animal model for the study of this condition, due to its anatomical similarities to humans. The aim of this study was to ex...
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2025-01-01
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| author | Agustín Cartes Caroll Stoore María Soledad Baquedano Christian Hidalgo Felipe Lillo Eduardo Landerer Galia Ramírez-Toloza Rodolfo Paredes |
| author_facet | Agustín Cartes Caroll Stoore María Soledad Baquedano Christian Hidalgo Felipe Lillo Eduardo Landerer Galia Ramírez-Toloza Rodolfo Paredes |
| author_sort | Agustín Cartes |
| collection | DOAJ |
| description | Urinary incontinence is a widespread issue, particularly among women, with effective treatments remaining elusive. The pig, and especially the female pig, stands as a promising animal model for the study of this condition, due to its anatomical similarities to humans. The aim of this study was to explore the largely uncharted muscular structure of the female pig urethra, linking urethral muscle dysfunction to incontinence. We examined histological sections from the urethras of six sows using Hematoxylin-Eosin and Masson’s trichrome staining for morphometric analysis. The statistical significance of cellular disposition was determined through analysis of variance (ANOVA), followed by a Tukey post hoc test to elucidate specific inter-group differences. Our analysis revealed segment-specific epithelial differences, including variations in cell layers, sparse acinar glands, rich vasculature, and distinct muscle fibers with diverse regional distributions. Notably, significant differences in muscular area and tissue distribution were identified between the proximal, middle, and distal segments of the urethra (<i>p</i> < 0.001). The observed anatomical variations, along with the cellular similarities between pigs and humans, establish the female pig as a crucial translational model for advancing urological research. Specifically, these findings provide a foundation for the development of innovative therapeutic strategies and surgical techniques that can be directly applied to improve outcomes in human urological conditions. |
| format | Article |
| id | doaj-art-d75e9101be71473984ea16e04d0dabc3 |
| institution | Kabale University |
| issn | 2079-7737 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-d75e9101be71473984ea16e04d0dabc32025-01-24T13:23:21ZengMDPI AGBiology2079-77372025-01-011413110.3390/biology14010031An Exploration of the Cellular Microenvironment of the Female Pig Urethra: Translational Insights for Urological ResearchAgustín Cartes0Caroll Stoore1María Soledad Baquedano2Christian Hidalgo3Felipe Lillo4Eduardo Landerer5Galia Ramírez-Toloza6Rodolfo Paredes7Escuela de Medicina Veterinaria, Facultad de Ciencias de la Vida, Universidad Andres Bello, República 239 3° Piso, Santiago 8370146, ChileEscuela de Medicina Veterinaria, Facultad de Ciencias de la Vida, Universidad Andres Bello, República 239 3° Piso, Santiago 8370146, ChileEscuela de Medicina Veterinaria, Facultad de Ciencias de la Vida, Universidad Andres Bello, República 239 3° Piso, Santiago 8370146, ChileNúcleo de Investigación en One Health (NIOH), Facultad de Medicina Veterinaria y Agronomía, Universidad de las Américas, Santiago 8370065, ChileEscuela de Medicina Veterinaria, Facultad de Ciencias de la Vida, Universidad Andres Bello, República 239 3° Piso, Santiago 8370146, ChileUnidad Urología, Centro de Cirugía Robótica, Clínica Indisa, Santa María 1810, Providencia, Santiago 7520440, ChileDepartamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santa Rosa 11735, La Pintana, Santiago 8820808, ChileEscuela de Medicina Veterinaria, Facultad de Ciencias de la Vida, Universidad Andres Bello, República 239 3° Piso, Santiago 8370146, ChileUrinary incontinence is a widespread issue, particularly among women, with effective treatments remaining elusive. The pig, and especially the female pig, stands as a promising animal model for the study of this condition, due to its anatomical similarities to humans. The aim of this study was to explore the largely uncharted muscular structure of the female pig urethra, linking urethral muscle dysfunction to incontinence. We examined histological sections from the urethras of six sows using Hematoxylin-Eosin and Masson’s trichrome staining for morphometric analysis. The statistical significance of cellular disposition was determined through analysis of variance (ANOVA), followed by a Tukey post hoc test to elucidate specific inter-group differences. Our analysis revealed segment-specific epithelial differences, including variations in cell layers, sparse acinar glands, rich vasculature, and distinct muscle fibers with diverse regional distributions. Notably, significant differences in muscular area and tissue distribution were identified between the proximal, middle, and distal segments of the urethra (<i>p</i> < 0.001). The observed anatomical variations, along with the cellular similarities between pigs and humans, establish the female pig as a crucial translational model for advancing urological research. Specifically, these findings provide a foundation for the development of innovative therapeutic strategies and surgical techniques that can be directly applied to improve outcomes in human urological conditions.https://www.mdpi.com/2079-7737/14/1/31pig urethraurethral anatomyurological modeltranslational research |
| spellingShingle | Agustín Cartes Caroll Stoore María Soledad Baquedano Christian Hidalgo Felipe Lillo Eduardo Landerer Galia Ramírez-Toloza Rodolfo Paredes An Exploration of the Cellular Microenvironment of the Female Pig Urethra: Translational Insights for Urological Research Biology pig urethra urethral anatomy urological model translational research |
| title | An Exploration of the Cellular Microenvironment of the Female Pig Urethra: Translational Insights for Urological Research |
| title_full | An Exploration of the Cellular Microenvironment of the Female Pig Urethra: Translational Insights for Urological Research |
| title_fullStr | An Exploration of the Cellular Microenvironment of the Female Pig Urethra: Translational Insights for Urological Research |
| title_full_unstemmed | An Exploration of the Cellular Microenvironment of the Female Pig Urethra: Translational Insights for Urological Research |
| title_short | An Exploration of the Cellular Microenvironment of the Female Pig Urethra: Translational Insights for Urological Research |
| title_sort | exploration of the cellular microenvironment of the female pig urethra translational insights for urological research |
| topic | pig urethra urethral anatomy urological model translational research |
| url | https://www.mdpi.com/2079-7737/14/1/31 |
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