An Optimized Anti-adherence and Anti-biofilm Assay: Case Study of Zinc Oxide Nanoparticles versus MRSA Biofilm
Biofilms form protective layers over bacteria that are associated with a majority of the hospital infections contributing to antibiotic resistance development in susceptible strains. Nowadays, there is a pressing need for developing effective anti-biofilm agents to help address the growing problem o...
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| Format: | Article |
| Language: | English |
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HH Publisher
2020-06-01
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| Series: | Progress in Microbes and Molecular Biology |
| Online Access: | https://journals.hh-publisher.com/index.php/pmmb/article/view/284 |
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| author | Hefa Mangzira Kemung Loh Teng-Hern Tan Kooi Yeong Khaw Yong Sze Ong Chim Kei Chan Darren Yi Sern Low Siah Ying Tang Bey-Hing Goh |
| author_facet | Hefa Mangzira Kemung Loh Teng-Hern Tan Kooi Yeong Khaw Yong Sze Ong Chim Kei Chan Darren Yi Sern Low Siah Ying Tang Bey-Hing Goh |
| author_sort | Hefa Mangzira Kemung |
| collection | DOAJ |
| description | Biofilms form protective layers over bacteria that are associated with a majority of the hospital infections contributing to antibiotic resistance development in susceptible strains. Nowadays, there is a pressing need for developing effective anti-biofilm agents to help address the growing problem of biofilm-producing bacteria associated with antibiotic resistance. In recent years, zinc oxide nanoparticles (ZnO-NPs) has emerged as a prospective candidate for new anti-biofilm agents. The present method paper described an optimized anti-adherence and anti-biofilm assay using ZnO-NPs. The antibiotic-resistant bacteria Methicillin-resistant Staphylococcus aureus (MRSA ATCC4330) and vancomycin were used as the growth control and positive control, respectively. The result showed concentration-dependent anti-adherence and antibiofilm activity. The ZnO-NPs effectively prevented attachment of bacterial cells onto walls of wells with 51.69 ± 2.55% at the highest concentration tested (65.4 µg/mL). ZnO-NPs was also able to break-up 50% pre-formed MRSA biofilm at the lowest concentration of 13.5 µg/mL. Interestingly, ZnO-NPs at lower concentrations demonstrated significantly stronger antibiofilm activity than that of the positive control vancomycin, demonstrating that ZnO-NPs is a promising anti-biofilm agent. This method could be used as a preliminary screening of transition metal oxide nanoparticles as potential anti-adherence and anti-biofilm agents followed by other specific anti-biofilm assays. |
| format | Article |
| id | doaj-art-d737dd9c754447bfa48f5ee3f18bc238 |
| institution | Kabale University |
| issn | 2637-1049 |
| language | English |
| publishDate | 2020-06-01 |
| publisher | HH Publisher |
| record_format | Article |
| series | Progress in Microbes and Molecular Biology |
| spelling | doaj-art-d737dd9c754447bfa48f5ee3f18bc2382025-02-04T08:37:03ZengHH PublisherProgress in Microbes and Molecular Biology2637-10492020-06-013110.36877/pmmb.a0000091An Optimized Anti-adherence and Anti-biofilm Assay: Case Study of Zinc Oxide Nanoparticles versus MRSA BiofilmHefa Mangzira KemungLoh Teng-Hern TanKooi Yeong KhawYong Sze OngChim Kei ChanDarren Yi Sern LowSiah Ying TangBey-Hing GohBiofilms form protective layers over bacteria that are associated with a majority of the hospital infections contributing to antibiotic resistance development in susceptible strains. Nowadays, there is a pressing need for developing effective anti-biofilm agents to help address the growing problem of biofilm-producing bacteria associated with antibiotic resistance. In recent years, zinc oxide nanoparticles (ZnO-NPs) has emerged as a prospective candidate for new anti-biofilm agents. The present method paper described an optimized anti-adherence and anti-biofilm assay using ZnO-NPs. The antibiotic-resistant bacteria Methicillin-resistant Staphylococcus aureus (MRSA ATCC4330) and vancomycin were used as the growth control and positive control, respectively. The result showed concentration-dependent anti-adherence and antibiofilm activity. The ZnO-NPs effectively prevented attachment of bacterial cells onto walls of wells with 51.69 ± 2.55% at the highest concentration tested (65.4 µg/mL). ZnO-NPs was also able to break-up 50% pre-formed MRSA biofilm at the lowest concentration of 13.5 µg/mL. Interestingly, ZnO-NPs at lower concentrations demonstrated significantly stronger antibiofilm activity than that of the positive control vancomycin, demonstrating that ZnO-NPs is a promising anti-biofilm agent. This method could be used as a preliminary screening of transition metal oxide nanoparticles as potential anti-adherence and anti-biofilm agents followed by other specific anti-biofilm assays.https://journals.hh-publisher.com/index.php/pmmb/article/view/284 |
| spellingShingle | Hefa Mangzira Kemung Loh Teng-Hern Tan Kooi Yeong Khaw Yong Sze Ong Chim Kei Chan Darren Yi Sern Low Siah Ying Tang Bey-Hing Goh An Optimized Anti-adherence and Anti-biofilm Assay: Case Study of Zinc Oxide Nanoparticles versus MRSA Biofilm Progress in Microbes and Molecular Biology |
| title | An Optimized Anti-adherence and Anti-biofilm Assay: Case Study of Zinc Oxide Nanoparticles versus MRSA Biofilm |
| title_full | An Optimized Anti-adherence and Anti-biofilm Assay: Case Study of Zinc Oxide Nanoparticles versus MRSA Biofilm |
| title_fullStr | An Optimized Anti-adherence and Anti-biofilm Assay: Case Study of Zinc Oxide Nanoparticles versus MRSA Biofilm |
| title_full_unstemmed | An Optimized Anti-adherence and Anti-biofilm Assay: Case Study of Zinc Oxide Nanoparticles versus MRSA Biofilm |
| title_short | An Optimized Anti-adherence and Anti-biofilm Assay: Case Study of Zinc Oxide Nanoparticles versus MRSA Biofilm |
| title_sort | optimized anti adherence and anti biofilm assay case study of zinc oxide nanoparticles versus mrsa biofilm |
| url | https://journals.hh-publisher.com/index.php/pmmb/article/view/284 |
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