Fluorescent Silica Nanoparticles in the Detection and Control of the Growth of Pathogen

In this present study the bioconjugated fluorescent silica nanoparticles give an efficient fluorescent-based immunoassay for the detection of pathogen. The synthesized silica nanoparticles were polydispersed and the size of the silica nanoparticles was in the range of 114–164 nm. The energy dispersi...

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Main Authors: Kethirabalan Chitra, Gurusamy Annadurai
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:Journal of Nanotechnology
Online Access:http://dx.doi.org/10.1155/2013/509628
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author Kethirabalan Chitra
Gurusamy Annadurai
author_facet Kethirabalan Chitra
Gurusamy Annadurai
author_sort Kethirabalan Chitra
collection DOAJ
description In this present study the bioconjugated fluorescent silica nanoparticles give an efficient fluorescent-based immunoassay for the detection of pathogen. The synthesized silica nanoparticles were polydispersed and the size of the silica nanoparticles was in the range of 114–164 nm. The energy dispersive X-ray spectrophotometer showed the presence of silica at 1.8 kev and the selected area diffractometer showed amorphous nature of silica nanoparticles. The FTIR spectrum confirmed the attachment of dye and carboxyl group onto the silica nanoparticles surface. The fluorescent silica nanoparticles showed highly efficient fluorescence and the fluorescent emission of silica nanoparticles occurred at 536 nm. The SEM image showed the aggregation of nanoparticles and bacteria. The growth of the pathogenic E. coli was controlled using silica nanoparticles; therefore silica nanoparticles could be used in food packaging material, biomedical material, and so forth. This work provides a rapid, simple, and accurate method for the detection of pathogen using fluorescent-based immunoassay.
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spelling doaj-art-d733e4c28bb34c40a01b3a094b23d1572025-02-03T01:28:37ZengWileyJournal of Nanotechnology1687-95031687-95112013-01-01201310.1155/2013/509628509628Fluorescent Silica Nanoparticles in the Detection and Control of the Growth of PathogenKethirabalan Chitra0Gurusamy Annadurai1Environmental Nanotechnology Division, Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi, Tamil Nadu 627412, IndiaEnvironmental Nanotechnology Division, Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi, Tamil Nadu 627412, IndiaIn this present study the bioconjugated fluorescent silica nanoparticles give an efficient fluorescent-based immunoassay for the detection of pathogen. The synthesized silica nanoparticles were polydispersed and the size of the silica nanoparticles was in the range of 114–164 nm. The energy dispersive X-ray spectrophotometer showed the presence of silica at 1.8 kev and the selected area diffractometer showed amorphous nature of silica nanoparticles. The FTIR spectrum confirmed the attachment of dye and carboxyl group onto the silica nanoparticles surface. The fluorescent silica nanoparticles showed highly efficient fluorescence and the fluorescent emission of silica nanoparticles occurred at 536 nm. The SEM image showed the aggregation of nanoparticles and bacteria. The growth of the pathogenic E. coli was controlled using silica nanoparticles; therefore silica nanoparticles could be used in food packaging material, biomedical material, and so forth. This work provides a rapid, simple, and accurate method for the detection of pathogen using fluorescent-based immunoassay.http://dx.doi.org/10.1155/2013/509628
spellingShingle Kethirabalan Chitra
Gurusamy Annadurai
Fluorescent Silica Nanoparticles in the Detection and Control of the Growth of Pathogen
Journal of Nanotechnology
title Fluorescent Silica Nanoparticles in the Detection and Control of the Growth of Pathogen
title_full Fluorescent Silica Nanoparticles in the Detection and Control of the Growth of Pathogen
title_fullStr Fluorescent Silica Nanoparticles in the Detection and Control of the Growth of Pathogen
title_full_unstemmed Fluorescent Silica Nanoparticles in the Detection and Control of the Growth of Pathogen
title_short Fluorescent Silica Nanoparticles in the Detection and Control of the Growth of Pathogen
title_sort fluorescent silica nanoparticles in the detection and control of the growth of pathogen
url http://dx.doi.org/10.1155/2013/509628
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