A Tentative Mechanism of Solubilization of Neuropathy Target Esterase from Chicken Embryo Brain by Phospholipase A2
The neuropathy target esterase is a membrane-bound enzyme linked to organophosphate-induced distal neuropathy. Here we report a tentative mechanism of its solubilization from chicken embryo brains by using phospholipase A2. The enzyme was released from brain membranes after degradation of their stru...
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| Format: | Article |
| Language: | English |
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Wiley
2008-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2008.51 |
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| _version_ | 1832556440669126656 |
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| author | Josef Seifert |
| author_facet | Josef Seifert |
| author_sort | Josef Seifert |
| collection | DOAJ |
| description | The neuropathy target esterase is a membrane-bound enzyme linked to organophosphate-induced distal neuropathy. Here we report a tentative mechanism of its solubilization from chicken embryo brains by using phospholipase A2. The enzyme was released from brain membranes after degradation of their structural phospholipids initiated by phospholipase A2. L-α-lysophosphatidylcholine, tested as a representative product of phospholipid hydrolysis, was identified as a new efficient detergent for solubilization of the neuropathy target esterase. |
| format | Article |
| id | doaj-art-d71e5f9eddb54bbea703d5e79b050bab |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2008-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-d71e5f9eddb54bbea703d5e79b050bab2025-02-03T05:45:27ZengWileyThe Scientific World Journal1537-744X2008-01-01834634910.1100/tsw.2008.51A Tentative Mechanism of Solubilization of Neuropathy Target Esterase from Chicken Embryo Brain by Phospholipase A2Josef Seifert0Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI 96822, USAThe neuropathy target esterase is a membrane-bound enzyme linked to organophosphate-induced distal neuropathy. Here we report a tentative mechanism of its solubilization from chicken embryo brains by using phospholipase A2. The enzyme was released from brain membranes after degradation of their structural phospholipids initiated by phospholipase A2. L-α-lysophosphatidylcholine, tested as a representative product of phospholipid hydrolysis, was identified as a new efficient detergent for solubilization of the neuropathy target esterase.http://dx.doi.org/10.1100/tsw.2008.51 |
| spellingShingle | Josef Seifert A Tentative Mechanism of Solubilization of Neuropathy Target Esterase from Chicken Embryo Brain by Phospholipase A2 The Scientific World Journal |
| title | A Tentative Mechanism of Solubilization of Neuropathy Target Esterase from Chicken Embryo Brain by Phospholipase A2 |
| title_full | A Tentative Mechanism of Solubilization of Neuropathy Target Esterase from Chicken Embryo Brain by Phospholipase A2 |
| title_fullStr | A Tentative Mechanism of Solubilization of Neuropathy Target Esterase from Chicken Embryo Brain by Phospholipase A2 |
| title_full_unstemmed | A Tentative Mechanism of Solubilization of Neuropathy Target Esterase from Chicken Embryo Brain by Phospholipase A2 |
| title_short | A Tentative Mechanism of Solubilization of Neuropathy Target Esterase from Chicken Embryo Brain by Phospholipase A2 |
| title_sort | tentative mechanism of solubilization of neuropathy target esterase from chicken embryo brain by phospholipase a2 |
| url | http://dx.doi.org/10.1100/tsw.2008.51 |
| work_keys_str_mv | AT josefseifert atentativemechanismofsolubilizationofneuropathytargetesterasefromchickenembryobrainbyphospholipasea2 AT josefseifert tentativemechanismofsolubilizationofneuropathytargetesterasefromchickenembryobrainbyphospholipasea2 |