Entamoeba histolytica: Gene Expression Analysis of Cells Invading Tissues
Entamoeba histolytica is a protozoan parasite that presents a risk to the health of millions of people worldwide. Due to the existence of different clinical forms caused by the parasite and also different virulence levels presented by one strain, one would expect differences in the profile of gene t...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/364264 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832546225141841920 |
|---|---|
| author | Helen C. Fernandes Ana F. Costa Michelle A. R. Freitas Almir S. Martins Jorge L. Pesquero Élida M. Rabelo Maria A. Gomes |
| author_facet | Helen C. Fernandes Ana F. Costa Michelle A. R. Freitas Almir S. Martins Jorge L. Pesquero Élida M. Rabelo Maria A. Gomes |
| author_sort | Helen C. Fernandes |
| collection | DOAJ |
| description | Entamoeba histolytica is a protozoan parasite that presents a risk to the health of millions of people worldwide. Due to the existence of different clinical forms caused by the parasite and also different virulence levels presented by one strain, one would expect differences in the profile of gene transcripts between virulent and nonvirulent cultures. In this study we used the differential display to select gene segments related to invasiveness of amoeba. One Brazilian strain of E. histolytica in two conditions, able or not to cause lesions in experimental animals, was used. RNA from this strain, was used to study the differential expression of genes. 29 specific gene fragments differentially expressed in the virulent strain were selected. By real-time PCR, six of these genes had confirmed their differential expression in the virulent culture. These genes may have important roles in triggering invasive amoebiasis and may be related to adaptation of trophozoites to difficulties encountered during colonization of the intestinal epithelium and liver tissue. Future studies with these genes may elucidate its actual role in tissue invasion by E. histolytica generating new pathways for diagnosis and treatment of amoebiasis. |
| format | Article |
| id | doaj-art-d710d0e5418a4b5f89a888a3fb2f340a |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-d710d0e5418a4b5f89a888a3fb2f340a2025-02-03T07:23:35ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/364264364264Entamoeba histolytica: Gene Expression Analysis of Cells Invading TissuesHelen C. Fernandes0Ana F. Costa1Michelle A. R. Freitas2Almir S. Martins3Jorge L. Pesquero4Élida M. Rabelo5Maria A. Gomes6Department of Parasitology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, BrazilDepartment of Parasitology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, BrazilLaboratory of Parasitology, Institute of Biomedical Sciences, Universidade Federal de Uberlândia, 38400-902 Uberlândia, MG, BrazilDepartment of Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, BrazilDepartment of Physiology and Biophysics, Institute of Biological Sciences, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, BrazilDepartment of Parasitology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, BrazilDepartment of Parasitology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, BrazilEntamoeba histolytica is a protozoan parasite that presents a risk to the health of millions of people worldwide. Due to the existence of different clinical forms caused by the parasite and also different virulence levels presented by one strain, one would expect differences in the profile of gene transcripts between virulent and nonvirulent cultures. In this study we used the differential display to select gene segments related to invasiveness of amoeba. One Brazilian strain of E. histolytica in two conditions, able or not to cause lesions in experimental animals, was used. RNA from this strain, was used to study the differential expression of genes. 29 specific gene fragments differentially expressed in the virulent strain were selected. By real-time PCR, six of these genes had confirmed their differential expression in the virulent culture. These genes may have important roles in triggering invasive amoebiasis and may be related to adaptation of trophozoites to difficulties encountered during colonization of the intestinal epithelium and liver tissue. Future studies with these genes may elucidate its actual role in tissue invasion by E. histolytica generating new pathways for diagnosis and treatment of amoebiasis.http://dx.doi.org/10.1155/2014/364264 |
| spellingShingle | Helen C. Fernandes Ana F. Costa Michelle A. R. Freitas Almir S. Martins Jorge L. Pesquero Élida M. Rabelo Maria A. Gomes Entamoeba histolytica: Gene Expression Analysis of Cells Invading Tissues The Scientific World Journal |
| title | Entamoeba histolytica: Gene Expression Analysis of Cells Invading Tissues |
| title_full | Entamoeba histolytica: Gene Expression Analysis of Cells Invading Tissues |
| title_fullStr | Entamoeba histolytica: Gene Expression Analysis of Cells Invading Tissues |
| title_full_unstemmed | Entamoeba histolytica: Gene Expression Analysis of Cells Invading Tissues |
| title_short | Entamoeba histolytica: Gene Expression Analysis of Cells Invading Tissues |
| title_sort | entamoeba histolytica gene expression analysis of cells invading tissues |
| url | http://dx.doi.org/10.1155/2014/364264 |
| work_keys_str_mv | AT helencfernandes entamoebahistolyticageneexpressionanalysisofcellsinvadingtissues AT anafcosta entamoebahistolyticageneexpressionanalysisofcellsinvadingtissues AT michellearfreitas entamoebahistolyticageneexpressionanalysisofcellsinvadingtissues AT almirsmartins entamoebahistolyticageneexpressionanalysisofcellsinvadingtissues AT jorgelpesquero entamoebahistolyticageneexpressionanalysisofcellsinvadingtissues AT elidamrabelo entamoebahistolyticageneexpressionanalysisofcellsinvadingtissues AT mariaagomes entamoebahistolyticageneexpressionanalysisofcellsinvadingtissues |