Effectiveness of ultra-/very-high-frequency oscillations combined with helium–oxygen gas mixture in a rabbit model
Abstract High-frequency oscillatory ventilation (HFOV) at frequencies of approximately 15 Hz is associated with optimal CO2 excretion. Higher frequencies using a nitrogen–oxygen gas mixture worsen CO2 excretion. An in vitro experiment using HFOV and a helium–oxygen gas mixture showed a significant i...
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Nature Portfolio
2024-10-01
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| Online Access: | https://doi.org/10.1038/s41598-024-77703-0 |
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| author | Louis Akiyama Shinobu Tatsunami Mieko Akita Naoki Shimizu |
| author_facet | Louis Akiyama Shinobu Tatsunami Mieko Akita Naoki Shimizu |
| author_sort | Louis Akiyama |
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| description | Abstract High-frequency oscillatory ventilation (HFOV) at frequencies of approximately 15 Hz is associated with optimal CO2 excretion. Higher frequencies using a nitrogen–oxygen gas mixture worsen CO2 excretion. An in vitro experiment using HFOV and a helium–oxygen gas mixture showed a significant increase in CO2 transport, which increased with increases in ventilation frequency. We hypothesised that in HFOV, the change in the arterial partial pressure of CO2 (PaCO2) would be greater at frequencies above 15 Hz when combined with helium–oxygen gas mixture administration. We tested this hypothesis in a hypoventilated healthy rabbit model by administering a helium–oxygen gas mixture at 15, 25, 35, and 45 Hz frequencies. One-way repeated measures ANOVA showed a significant decrease in PaCO2 among the four ventilation frequency groups. Post-hoc analysis showed significant differences between 15 and 35 Hz frequencies and between 15 and 45 Hz frequencies. The mean (standard error) decrease of PaCO2 was 10.8 (2.2), 14.1 (2.3), 21.3 (3.3), and 23.1 (2.5) mmHg at 15, 25, 35, and 45 Hz, respectively. Combination therapy of helium–oxygen gas mixture and high-frequency oscillation using ultra/very high frequencies (35–45 Hz) was associated with a greater PaCO2 decrease than that using the standard frequency (15 Hz). |
| format | Article |
| id | doaj-art-d70e6524eb4a4787b2863b78695d95d3 |
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| issn | 2045-2322 |
| language | English |
| publishDate | 2024-10-01 |
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| spelling | doaj-art-d70e6524eb4a4787b2863b78695d95d32025-08-20T02:18:24ZengNature PortfolioScientific Reports2045-23222024-10-0114111010.1038/s41598-024-77703-0Effectiveness of ultra-/very-high-frequency oscillations combined with helium–oxygen gas mixture in a rabbit modelLouis Akiyama0Shinobu Tatsunami1Mieko Akita2Naoki Shimizu3Department of Paediatrics, St. Marianna University School of MedicineDepartment of Paediatrics, St. Marianna University School of MedicineDepartment of Paediatrics, St. Marianna University School of MedicineDepartment of Paediatrics, St. Marianna University School of MedicineAbstract High-frequency oscillatory ventilation (HFOV) at frequencies of approximately 15 Hz is associated with optimal CO2 excretion. Higher frequencies using a nitrogen–oxygen gas mixture worsen CO2 excretion. An in vitro experiment using HFOV and a helium–oxygen gas mixture showed a significant increase in CO2 transport, which increased with increases in ventilation frequency. We hypothesised that in HFOV, the change in the arterial partial pressure of CO2 (PaCO2) would be greater at frequencies above 15 Hz when combined with helium–oxygen gas mixture administration. We tested this hypothesis in a hypoventilated healthy rabbit model by administering a helium–oxygen gas mixture at 15, 25, 35, and 45 Hz frequencies. One-way repeated measures ANOVA showed a significant decrease in PaCO2 among the four ventilation frequency groups. Post-hoc analysis showed significant differences between 15 and 35 Hz frequencies and between 15 and 45 Hz frequencies. The mean (standard error) decrease of PaCO2 was 10.8 (2.2), 14.1 (2.3), 21.3 (3.3), and 23.1 (2.5) mmHg at 15, 25, 35, and 45 Hz, respectively. Combination therapy of helium–oxygen gas mixture and high-frequency oscillation using ultra/very high frequencies (35–45 Hz) was associated with a greater PaCO2 decrease than that using the standard frequency (15 Hz).https://doi.org/10.1038/s41598-024-77703-0High frequency oscillatory ventilationHeliumUltra-high frequencyCO2 excretionOscillationAnimal experiment |
| spellingShingle | Louis Akiyama Shinobu Tatsunami Mieko Akita Naoki Shimizu Effectiveness of ultra-/very-high-frequency oscillations combined with helium–oxygen gas mixture in a rabbit model Scientific Reports High frequency oscillatory ventilation Helium Ultra-high frequency CO2 excretion Oscillation Animal experiment |
| title | Effectiveness of ultra-/very-high-frequency oscillations combined with helium–oxygen gas mixture in a rabbit model |
| title_full | Effectiveness of ultra-/very-high-frequency oscillations combined with helium–oxygen gas mixture in a rabbit model |
| title_fullStr | Effectiveness of ultra-/very-high-frequency oscillations combined with helium–oxygen gas mixture in a rabbit model |
| title_full_unstemmed | Effectiveness of ultra-/very-high-frequency oscillations combined with helium–oxygen gas mixture in a rabbit model |
| title_short | Effectiveness of ultra-/very-high-frequency oscillations combined with helium–oxygen gas mixture in a rabbit model |
| title_sort | effectiveness of ultra very high frequency oscillations combined with helium oxygen gas mixture in a rabbit model |
| topic | High frequency oscillatory ventilation Helium Ultra-high frequency CO2 excretion Oscillation Animal experiment |
| url | https://doi.org/10.1038/s41598-024-77703-0 |
| work_keys_str_mv | AT louisakiyama effectivenessofultraveryhighfrequencyoscillationscombinedwithheliumoxygengasmixtureinarabbitmodel AT shinobutatsunami effectivenessofultraveryhighfrequencyoscillationscombinedwithheliumoxygengasmixtureinarabbitmodel AT miekoakita effectivenessofultraveryhighfrequencyoscillationscombinedwithheliumoxygengasmixtureinarabbitmodel AT naokishimizu effectivenessofultraveryhighfrequencyoscillationscombinedwithheliumoxygengasmixtureinarabbitmodel |