NKG2D Signaling Leads to NK Cell Mediated Lysis of Childhood AML
Natural killer cells have been shown to be relevant in the recognition and lysis of acute myeloid leukemia. In childhood acute lymphoblastic leukemia, it was shown that HLA I expression and KIR receptor-ligand mismatch significantly impact ALL cytolysis. We characterized 14 different primary childho...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/473175 |
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| _version_ | 1832545816491851776 |
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| author | Patrick Schlegel Kerstin Ditthard Peter Lang Markus Mezger Sebastian Michaelis Rupert Handgretinger Matthias Pfeiffer |
| author_facet | Patrick Schlegel Kerstin Ditthard Peter Lang Markus Mezger Sebastian Michaelis Rupert Handgretinger Matthias Pfeiffer |
| author_sort | Patrick Schlegel |
| collection | DOAJ |
| description | Natural killer cells have been shown to be relevant in the recognition and lysis of acute myeloid leukemia. In childhood acute lymphoblastic leukemia, it was shown that HLA I expression and KIR receptor-ligand mismatch significantly impact ALL cytolysis. We characterized 14 different primary childhood AML blasts by flow cytometry including NKG2D ligands. Further HLA I typing of blasts was performed and HLA I on the AML blasts was quantified. In two healthy volunteer NK cell donors HLA I typing and KIR genotyping were done. Blasts with high NKG2D ligand expression had significantly higher lysis by isolated NK cells. Grouping the blasts by NKG2D ligand expression led to a significant inverse correlation of HLA I expression and cytolysis in NKG2D low blasts. Furthermore, a significant positive correlation of NKG2D ligand expression and blast cytolysis was shown. No impact of KIR ligand-ligand mismatch was found but a significantly increased lysis of homozygous C2 blasts by KIR2DL1 negative NK cells (donor B) was revealed. In conclusion, NKG2D signaling leads to NK cell mediated lysis of childhood AML despite high HLA I expression. |
| format | Article |
| id | doaj-art-d6f6007238ea4bf884ce03e9fd6db61e |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-d6f6007238ea4bf884ce03e9fd6db61e2025-02-03T07:24:38ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/473175473175NKG2D Signaling Leads to NK Cell Mediated Lysis of Childhood AMLPatrick Schlegel0Kerstin Ditthard1Peter Lang2Markus Mezger3Sebastian Michaelis4Rupert Handgretinger5Matthias Pfeiffer6Department of Hematology and Oncology, University Children’s Hospital Tübingen, University of Tübingen, Hoppe-Seyler-Straße 1, 72076 Tübingen, GermanyDepartment of Hematology and Oncology, University Children’s Hospital Tübingen, University of Tübingen, Hoppe-Seyler-Straße 1, 72076 Tübingen, GermanyDepartment of Hematology and Oncology, University Children’s Hospital Tübingen, University of Tübingen, Hoppe-Seyler-Straße 1, 72076 Tübingen, GermanyDepartment of Hematology and Oncology, University Children’s Hospital Tübingen, University of Tübingen, Hoppe-Seyler-Straße 1, 72076 Tübingen, GermanyDepartment of Hematology and Oncology, University Children’s Hospital Tübingen, University of Tübingen, Hoppe-Seyler-Straße 1, 72076 Tübingen, GermanyDepartment of Hematology and Oncology, University Children’s Hospital Tübingen, University of Tübingen, Hoppe-Seyler-Straße 1, 72076 Tübingen, GermanyDepartment of Hematology and Oncology, University Children’s Hospital Tübingen, University of Tübingen, Hoppe-Seyler-Straße 1, 72076 Tübingen, GermanyNatural killer cells have been shown to be relevant in the recognition and lysis of acute myeloid leukemia. In childhood acute lymphoblastic leukemia, it was shown that HLA I expression and KIR receptor-ligand mismatch significantly impact ALL cytolysis. We characterized 14 different primary childhood AML blasts by flow cytometry including NKG2D ligands. Further HLA I typing of blasts was performed and HLA I on the AML blasts was quantified. In two healthy volunteer NK cell donors HLA I typing and KIR genotyping were done. Blasts with high NKG2D ligand expression had significantly higher lysis by isolated NK cells. Grouping the blasts by NKG2D ligand expression led to a significant inverse correlation of HLA I expression and cytolysis in NKG2D low blasts. Furthermore, a significant positive correlation of NKG2D ligand expression and blast cytolysis was shown. No impact of KIR ligand-ligand mismatch was found but a significantly increased lysis of homozygous C2 blasts by KIR2DL1 negative NK cells (donor B) was revealed. In conclusion, NKG2D signaling leads to NK cell mediated lysis of childhood AML despite high HLA I expression.http://dx.doi.org/10.1155/2015/473175 |
| spellingShingle | Patrick Schlegel Kerstin Ditthard Peter Lang Markus Mezger Sebastian Michaelis Rupert Handgretinger Matthias Pfeiffer NKG2D Signaling Leads to NK Cell Mediated Lysis of Childhood AML Journal of Immunology Research |
| title | NKG2D Signaling Leads to NK Cell Mediated Lysis of Childhood AML |
| title_full | NKG2D Signaling Leads to NK Cell Mediated Lysis of Childhood AML |
| title_fullStr | NKG2D Signaling Leads to NK Cell Mediated Lysis of Childhood AML |
| title_full_unstemmed | NKG2D Signaling Leads to NK Cell Mediated Lysis of Childhood AML |
| title_short | NKG2D Signaling Leads to NK Cell Mediated Lysis of Childhood AML |
| title_sort | nkg2d signaling leads to nk cell mediated lysis of childhood aml |
| url | http://dx.doi.org/10.1155/2015/473175 |
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