Blood Glucose Lowering Efficacy of Strawberry Extracts Rich in Ellagitannins with Different Degree of Polymerization in Rats
In two experiments on rats it was analyzed whether the lowering rate of postprandial glycaemia depends on the degree of polymerization of strawberry ellagitannins (ET). Two types of highly-concentrated polyphenolic extracts were used, one rich in monomeric ET (ETM; 94.9% polyphenols and 67% ETM in i...
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Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2016-06-01
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| Series: | Polish Journal of Food and Nutrition Sciences |
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| Online Access: | http://www.degruyter.com/view/j/pjfns.2016.66.issue-2/pjfns-2016-0001/pjfns-2016-0001.xml?format=INT |
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| author | Juśkiewicz Jerzy Jurgonski Adam Kołodziejczyk Krzysztof Kosmala Monika Milala Joanna Zduńczyk Zenon Fotschki Bartosz Żary-Sikorska Ewa |
| author_facet | Juśkiewicz Jerzy Jurgonski Adam Kołodziejczyk Krzysztof Kosmala Monika Milala Joanna Zduńczyk Zenon Fotschki Bartosz Żary-Sikorska Ewa |
| author_sort | Juśkiewicz Jerzy |
| collection | DOAJ |
| description | In two experiments on rats it was analyzed whether the lowering rate of postprandial glycaemia depends on the degree of polymerization of strawberry ellagitannins (ET). Two types of highly-concentrated polyphenolic extracts were used, one rich in monomeric ET (ETM; 94.9% polyphenols and 67% ETM in it), the second in dimeric ET (ETD; 95.9% polyphenols, and 78.0% ETD in it). The experiment 1 comprised three oral sugar tolerance tests, GTT (with glucose; to examine monosaccharide transport across epithelium), SuTT (with sucrose; to examine the activity of mucosal disaccharidase) and StTT (with starch; to examine the action of salivary alpha-amylase, and then of pancreatic amylase in the small intestine as well as all the aforementioned mechanisms), while in the experiment 2 the healthy and pre-diabetic rats were subjected to 1-h small intestine perfusion with fluids containing glucose or sucrose. Considering the shape of blood glucose concentration curve following GTT and SuTT, as well as changes in glycaemia and mucosal disaccharidases activity observed within and after small intestine perfusion, the ETM (vs. ETD) extract more positively mitigated sugar-induced post-prandial glycaemic load. Contrary to expectations, the effect of extracts on pre-diabetic rats was negligible. The extract ETD, in turn, potently mitigated starch-induced postprandial glycaemic load during test StTT. The results clearly showed that the separate mechanisms (glucose transport across the intestine, intestinal mucosal disaccharidases inhibition activity, pancreatic α-amylase inhibitory activity) leading to decreased postprandial glycaemia upon dietary administration of strawberry ellagitannins largely depended on their chemical structure, i.e. degree of polymerization. |
| format | Article |
| id | doaj-art-d6f3a955e8fa4eafb5ff876fac2b5b11 |
| institution | Kabale University |
| issn | 2083-6007 |
| language | English |
| publishDate | 2016-06-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
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| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj-art-d6f3a955e8fa4eafb5ff876fac2b5b112025-02-02T15:26:06ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072016-06-0166210911810.1515/pjfns-2016-0001pjfns-2016-0001Blood Glucose Lowering Efficacy of Strawberry Extracts Rich in Ellagitannins with Different Degree of Polymerization in RatsJuśkiewicz Jerzy0Jurgonski Adam1Kołodziejczyk Krzysztof2Kosmala Monika3Milala Joanna4Zduńczyk Zenon5Fotschki Bartosz6Żary-Sikorska Ewa7Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Division of Food Science, Tuwima 10, 10–748 Olsztyn, PolandInstitute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Division of Food Science, Tuwima 10, 10–748 Olsztyn, PolandInstitute of Food Technology and Analysis, Łódź University of Technology, Stefanowskiego 4/10, 90–924 Łódź, PolandInstitute of Food Technology and Analysis, Łódź University of Technology, Stefanowskiego 4/10, 90–924 Łódź, PolandInstitute of Food Technology and Analysis, Łódź University of Technology, Stefanowskiego 4/10, 90–924 Łódź, PolandInstitute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Division of Food Science, Tuwima 10, 10–748 Olsztyn, PolandInstitute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Division of Food Science, Tuwima 10, 10–748 Olsztyn, PolandUniversity of Science and Technology, Bernardyńska 6/8, Bydgoszcz, PolandIn two experiments on rats it was analyzed whether the lowering rate of postprandial glycaemia depends on the degree of polymerization of strawberry ellagitannins (ET). Two types of highly-concentrated polyphenolic extracts were used, one rich in monomeric ET (ETM; 94.9% polyphenols and 67% ETM in it), the second in dimeric ET (ETD; 95.9% polyphenols, and 78.0% ETD in it). The experiment 1 comprised three oral sugar tolerance tests, GTT (with glucose; to examine monosaccharide transport across epithelium), SuTT (with sucrose; to examine the activity of mucosal disaccharidase) and StTT (with starch; to examine the action of salivary alpha-amylase, and then of pancreatic amylase in the small intestine as well as all the aforementioned mechanisms), while in the experiment 2 the healthy and pre-diabetic rats were subjected to 1-h small intestine perfusion with fluids containing glucose or sucrose. Considering the shape of blood glucose concentration curve following GTT and SuTT, as well as changes in glycaemia and mucosal disaccharidases activity observed within and after small intestine perfusion, the ETM (vs. ETD) extract more positively mitigated sugar-induced post-prandial glycaemic load. Contrary to expectations, the effect of extracts on pre-diabetic rats was negligible. The extract ETD, in turn, potently mitigated starch-induced postprandial glycaemic load during test StTT. The results clearly showed that the separate mechanisms (glucose transport across the intestine, intestinal mucosal disaccharidases inhibition activity, pancreatic α-amylase inhibitory activity) leading to decreased postprandial glycaemia upon dietary administration of strawberry ellagitannins largely depended on their chemical structure, i.e. degree of polymerization.http://www.degruyter.com/view/j/pjfns.2016.66.issue-2/pjfns-2016-0001/pjfns-2016-0001.xml?format=INTstrawberryellagitanninsdegree of polymerizationpostprandial glycaemiarat |
| spellingShingle | Juśkiewicz Jerzy Jurgonski Adam Kołodziejczyk Krzysztof Kosmala Monika Milala Joanna Zduńczyk Zenon Fotschki Bartosz Żary-Sikorska Ewa Blood Glucose Lowering Efficacy of Strawberry Extracts Rich in Ellagitannins with Different Degree of Polymerization in Rats Polish Journal of Food and Nutrition Sciences strawberry ellagitannins degree of polymerization postprandial glycaemia rat |
| title | Blood Glucose Lowering Efficacy of Strawberry Extracts Rich in Ellagitannins with Different Degree of Polymerization in Rats |
| title_full | Blood Glucose Lowering Efficacy of Strawberry Extracts Rich in Ellagitannins with Different Degree of Polymerization in Rats |
| title_fullStr | Blood Glucose Lowering Efficacy of Strawberry Extracts Rich in Ellagitannins with Different Degree of Polymerization in Rats |
| title_full_unstemmed | Blood Glucose Lowering Efficacy of Strawberry Extracts Rich in Ellagitannins with Different Degree of Polymerization in Rats |
| title_short | Blood Glucose Lowering Efficacy of Strawberry Extracts Rich in Ellagitannins with Different Degree of Polymerization in Rats |
| title_sort | blood glucose lowering efficacy of strawberry extracts rich in ellagitannins with different degree of polymerization in rats |
| topic | strawberry ellagitannins degree of polymerization postprandial glycaemia rat |
| url | http://www.degruyter.com/view/j/pjfns.2016.66.issue-2/pjfns-2016-0001/pjfns-2016-0001.xml?format=INT |
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