Dysregulated T Cell Activation and Aberrant Cytokine Expression Profile in Systemic Lupus Erythematosus
Accumulating evidence indicates a critical role for T cells and relevant cytokines in the pathogenesis of systemic lupus erythematosus (SLE). However, the specific contribution of T cells together with the related circulating cytokines in disease pathogenesis and organ involvement is still not clear...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2019/8450947 |
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| author | Haiyan Zhou Bojiang Li Jing Li Tongqian Wu Xiaoqian Jin Rui Yuan Ping Shi Yan Zhou Long Li Fang Yu |
| author_facet | Haiyan Zhou Bojiang Li Jing Li Tongqian Wu Xiaoqian Jin Rui Yuan Ping Shi Yan Zhou Long Li Fang Yu |
| author_sort | Haiyan Zhou |
| collection | DOAJ |
| description | Accumulating evidence indicates a critical role for T cells and relevant cytokines in the pathogenesis of systemic lupus erythematosus (SLE). However, the specific contribution of T cells together with the related circulating cytokines in disease pathogenesis and organ involvement is still not clear. In the current study, we investigated relevant molecule expressions and cytokine levels in blood samples from 49 SLE patients and 22 healthy control subjects. The expression of HLA-DR and costimulatory molecules on T cells was evaluated by flow cytometry. Concentrations of serum C-reactive protein, erythrocyte sedimentation rate, anti-double-stranded DNA (anti-dsDNA) antibody, total lgG, complement 3, and complement 4 were measured. Serum cytokines and chemokines were measured by a cytometric bead array assay. Elevated frequencies of HLA-DR+ T cells and ICOS+ T cells were observed in SLE patients with positive anti-dsDNA antibodies compared with those in healthy controls (P<0.001). The expression of HLA-DR+ T cells was positively correlated with SLEDAI (r=0.15, P<0.01). Furthermore, levels of serum IL-6, MCP-1, TNFRI, IL-10, IL-12, and CCL20 were higher in SLE patients compared with healthy controls. In addition, patients with hematologic manifestations displayed elevated frequencies of HLA-DR+ T cells and ICOS+ T cells. Patients with renal manifestations had a decreased frequency of TIGIT+ T cells. These results suggested a dysregulated T cell activity and cytokine expression profiles in SLE subjects. We also developed a chemokine and cytokine profiling strategy to predict the activity of SLE, which has clinical implication for better monitoring the flares and remission during the course of SLE and for assessing therapeutic interventions. |
| format | Article |
| id | doaj-art-d6dbcea076074ab9b8892620f3354fa1 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-d6dbcea076074ab9b8892620f3354fa12025-02-03T01:29:16ZengWileyMediators of Inflammation0962-93511466-18612019-01-01201910.1155/2019/84509478450947Dysregulated T Cell Activation and Aberrant Cytokine Expression Profile in Systemic Lupus ErythematosusHaiyan Zhou0Bojiang Li1Jing Li2Tongqian Wu3Xiaoqian Jin4Rui Yuan5Ping Shi6Yan Zhou7Long Li8Fang Yu9Clinical Research Center, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, ChinaDepartment of Immunology and Rheumatology, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, ChinaClinical Laboratory Center, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, ChinaClinical Research Center, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, ChinaGuizhou Medical University, Guiyang 550004, ChinaClinical Laboratory Center, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, ChinaClinical Laboratory Center, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, ChinaClinical Laboratory Center, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, ChinaDepartment of Immunology and Rheumatology, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, ChinaClinical Research Center, The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, ChinaAccumulating evidence indicates a critical role for T cells and relevant cytokines in the pathogenesis of systemic lupus erythematosus (SLE). However, the specific contribution of T cells together with the related circulating cytokines in disease pathogenesis and organ involvement is still not clear. In the current study, we investigated relevant molecule expressions and cytokine levels in blood samples from 49 SLE patients and 22 healthy control subjects. The expression of HLA-DR and costimulatory molecules on T cells was evaluated by flow cytometry. Concentrations of serum C-reactive protein, erythrocyte sedimentation rate, anti-double-stranded DNA (anti-dsDNA) antibody, total lgG, complement 3, and complement 4 were measured. Serum cytokines and chemokines were measured by a cytometric bead array assay. Elevated frequencies of HLA-DR+ T cells and ICOS+ T cells were observed in SLE patients with positive anti-dsDNA antibodies compared with those in healthy controls (P<0.001). The expression of HLA-DR+ T cells was positively correlated with SLEDAI (r=0.15, P<0.01). Furthermore, levels of serum IL-6, MCP-1, TNFRI, IL-10, IL-12, and CCL20 were higher in SLE patients compared with healthy controls. In addition, patients with hematologic manifestations displayed elevated frequencies of HLA-DR+ T cells and ICOS+ T cells. Patients with renal manifestations had a decreased frequency of TIGIT+ T cells. These results suggested a dysregulated T cell activity and cytokine expression profiles in SLE subjects. We also developed a chemokine and cytokine profiling strategy to predict the activity of SLE, which has clinical implication for better monitoring the flares and remission during the course of SLE and for assessing therapeutic interventions.http://dx.doi.org/10.1155/2019/8450947 |
| spellingShingle | Haiyan Zhou Bojiang Li Jing Li Tongqian Wu Xiaoqian Jin Rui Yuan Ping Shi Yan Zhou Long Li Fang Yu Dysregulated T Cell Activation and Aberrant Cytokine Expression Profile in Systemic Lupus Erythematosus Mediators of Inflammation |
| title | Dysregulated T Cell Activation and Aberrant Cytokine Expression Profile in Systemic Lupus Erythematosus |
| title_full | Dysregulated T Cell Activation and Aberrant Cytokine Expression Profile in Systemic Lupus Erythematosus |
| title_fullStr | Dysregulated T Cell Activation and Aberrant Cytokine Expression Profile in Systemic Lupus Erythematosus |
| title_full_unstemmed | Dysregulated T Cell Activation and Aberrant Cytokine Expression Profile in Systemic Lupus Erythematosus |
| title_short | Dysregulated T Cell Activation and Aberrant Cytokine Expression Profile in Systemic Lupus Erythematosus |
| title_sort | dysregulated t cell activation and aberrant cytokine expression profile in systemic lupus erythematosus |
| url | http://dx.doi.org/10.1155/2019/8450947 |
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