Correlation between selenium levels and selenoproteins expression in idiopathic generalized epilepsy: a study from Karachi
Abstract Background Oxidative damage has been implicated in multiple neurodegenerative diseases, including epilepsy. Selenium, in the form of selenoproteins is an integral part of the human antioxidant defense system. Though a relationship between the altered selenium levels and epilepsy has been re...
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BMC
2025-01-01
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| Series: | BMC Neurology |
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| Online Access: | https://doi.org/10.1186/s12883-024-03993-6 |
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| author | Hareem Nisar Rafat Amin Sadaf Khan Tehseen Fatima Qamar-Un-Nisa Jawwad-Us-Salam |
| author_facet | Hareem Nisar Rafat Amin Sadaf Khan Tehseen Fatima Qamar-Un-Nisa Jawwad-Us-Salam |
| author_sort | Hareem Nisar |
| collection | DOAJ |
| description | Abstract Background Oxidative damage has been implicated in multiple neurodegenerative diseases, including epilepsy. Selenium, in the form of selenoproteins is an integral part of the human antioxidant defense system. Though a relationship between the altered selenium levels and epilepsy has been reported, limited evidence is available about the expression pattern of selenoproteins in epileptic patients. Objective This study aimed to determine the serum selenium levels in idiopathic epileptic and healthy individuals. Expression profiling of selenoproteins (GPx1, TRxR1 and SEPW1) both at mRNA and protein levels was also evaluated. Methods Serum selenium levels of 30 patients with idiopathic generalized epilepsy and their age and gender matched 30 healthy controls were measured. Protein levels of Serum Glutathione Peroxidase 1 (GPx1), Thioredoxin Reductase 1 (TRxR1) and Selenoprotein W (SEPW1) were estimated using ELISA. mRNA expression of GPx1, TRxR1 and SEPW1 were determined using qRT-PCR. Results The mean values for serum selenium levels in cases and controls were 37.6 ± 2.0 µmol/ml and 38.9 ± 2.7 µmol/ml, respectively. Selenium levels in cases were significantly lower as compared to controls (p = 0.031). No statistically significant differences were observed between the serum levels of selenoproteins GPx1, TRxR1 and SEPW1 in epileptic patients and the healthy group. GPx1 and TRxR1 expression was found to be down regulated (0.34 and 0.13 folds respectively) whereas SEPW 1 was found to be 0.04 folds up regulated in epileptic patients compared to the healthy subjects. Conclusion Selenium deficiency observed in epileptic patients suggests the association between serum selenium levels and epilepsy. This study provides the information about the selenium status in Pakistani population and helps in understanding the role of selenium in the prevention of epilepsy. Graphical Abstract |
| format | Article |
| id | doaj-art-d6d664313d974c42a2fdc8a3b9378dbf |
| institution | Kabale University |
| issn | 1471-2377 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Neurology |
| spelling | doaj-art-d6d664313d974c42a2fdc8a3b9378dbf2025-01-26T12:39:24ZengBMCBMC Neurology1471-23772025-01-0125111610.1186/s12883-024-03993-6Correlation between selenium levels and selenoproteins expression in idiopathic generalized epilepsy: a study from KarachiHareem Nisar0Rafat Amin1Sadaf Khan2Tehseen Fatima3Qamar-Un-Nisa4Jawwad-Us-Salam5Sindh Institute of Medical Sciences, Sindh Institute Of Urology And TransplantationDow College of Biotechnology, Dow University of Health SciencesDow College of Biotechnology, Dow University of Health SciencesDow College of Biotechnology, Dow University of Health SciencesDepartment of Neurology, Dr. Ruth K. M. Pfau Civil Hospital, Dow University of Health SciencesDepartment of Neurology, Dow University Hospital, Dow University of health sciencesAbstract Background Oxidative damage has been implicated in multiple neurodegenerative diseases, including epilepsy. Selenium, in the form of selenoproteins is an integral part of the human antioxidant defense system. Though a relationship between the altered selenium levels and epilepsy has been reported, limited evidence is available about the expression pattern of selenoproteins in epileptic patients. Objective This study aimed to determine the serum selenium levels in idiopathic epileptic and healthy individuals. Expression profiling of selenoproteins (GPx1, TRxR1 and SEPW1) both at mRNA and protein levels was also evaluated. Methods Serum selenium levels of 30 patients with idiopathic generalized epilepsy and their age and gender matched 30 healthy controls were measured. Protein levels of Serum Glutathione Peroxidase 1 (GPx1), Thioredoxin Reductase 1 (TRxR1) and Selenoprotein W (SEPW1) were estimated using ELISA. mRNA expression of GPx1, TRxR1 and SEPW1 were determined using qRT-PCR. Results The mean values for serum selenium levels in cases and controls were 37.6 ± 2.0 µmol/ml and 38.9 ± 2.7 µmol/ml, respectively. Selenium levels in cases were significantly lower as compared to controls (p = 0.031). No statistically significant differences were observed between the serum levels of selenoproteins GPx1, TRxR1 and SEPW1 in epileptic patients and the healthy group. GPx1 and TRxR1 expression was found to be down regulated (0.34 and 0.13 folds respectively) whereas SEPW 1 was found to be 0.04 folds up regulated in epileptic patients compared to the healthy subjects. Conclusion Selenium deficiency observed in epileptic patients suggests the association between serum selenium levels and epilepsy. This study provides the information about the selenium status in Pakistani population and helps in understanding the role of selenium in the prevention of epilepsy. Graphical Abstracthttps://doi.org/10.1186/s12883-024-03993-6SeleniumSelenoproteinsGPx1TRxR1SEPW1QRT-PCR |
| spellingShingle | Hareem Nisar Rafat Amin Sadaf Khan Tehseen Fatima Qamar-Un-Nisa Jawwad-Us-Salam Correlation between selenium levels and selenoproteins expression in idiopathic generalized epilepsy: a study from Karachi BMC Neurology Selenium Selenoproteins GPx1 TRxR1 SEPW1 QRT-PCR |
| title | Correlation between selenium levels and selenoproteins expression in idiopathic generalized epilepsy: a study from Karachi |
| title_full | Correlation between selenium levels and selenoproteins expression in idiopathic generalized epilepsy: a study from Karachi |
| title_fullStr | Correlation between selenium levels and selenoproteins expression in idiopathic generalized epilepsy: a study from Karachi |
| title_full_unstemmed | Correlation between selenium levels and selenoproteins expression in idiopathic generalized epilepsy: a study from Karachi |
| title_short | Correlation between selenium levels and selenoproteins expression in idiopathic generalized epilepsy: a study from Karachi |
| title_sort | correlation between selenium levels and selenoproteins expression in idiopathic generalized epilepsy a study from karachi |
| topic | Selenium Selenoproteins GPx1 TRxR1 SEPW1 QRT-PCR |
| url | https://doi.org/10.1186/s12883-024-03993-6 |
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