Can Gut Microbiota Analysis Reveal <i>Clostridioides difficile</i> Infection? Evidence from an Italian Cohort at Disease Onset
A diverse and well-functioning gut microbiota normally serves as a protective shield against the invasion of harmful bacteria or the proliferation of opportunistic pathogens. <i>Clostridioides difficile</i> infection (CDI) is predominantly associated with the overuse of antibiotics, resu...
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2024-12-01
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| author | Roberto Rosato Gianluca Quaranta Giulia Santarelli Giovanni Fancello Delia Mercedes Bianco Francesca Romana Monzo Stefano Bibbò Giovanni Cammarota Maurizio Sanguinetti Luca Masucci Flavio De Maio |
| author_facet | Roberto Rosato Gianluca Quaranta Giulia Santarelli Giovanni Fancello Delia Mercedes Bianco Francesca Romana Monzo Stefano Bibbò Giovanni Cammarota Maurizio Sanguinetti Luca Masucci Flavio De Maio |
| author_sort | Roberto Rosato |
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| description | A diverse and well-functioning gut microbiota normally serves as a protective shield against the invasion of harmful bacteria or the proliferation of opportunistic pathogens. <i>Clostridioides difficile</i> infection (CDI) is predominantly associated with the overuse of antibiotics, resulting in a significant alteration in the gut’s microbial balance. Unfortunately, the lack of global standardization does not allow for the identification of a set of biomarkers associated with the onset and progression of this disease. In this study, we examined the composition of the gut microbiota in patients at the time of the initial detection of CDI compared to a control group of CDI-negative individuals, with a focus on identifying potential CDI biomarkers for diagnosis. While no significant differences in the alpha and beta diversity between CDI-negative and CDI-positive individuals were found, we found that certain genera (such as <i>Clostridium</i> XIVa and <i>Clostridium</i> XVIII) showed different abundance patterns in the two groups, indicating potential differences in gut microbiota balance. In conclusion, am enrichment in <i>Clostridium</i> XI and a decrease in <i>Faecalibacterium</i> emerged in the CDI-positive patients and following antibiotic treatment, indicating that changes in the <i>Clostridium/Faecalibacterium</i> ratio may be a promising biomarker that warrants further investigation for CDI diagnosis. |
| format | Article |
| id | doaj-art-d6d38a766e3a4d248a2ffa97e377319e |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-d6d38a766e3a4d248a2ffa97e377319e2025-01-24T13:42:18ZengMDPI AGMicroorganisms2076-26072024-12-011311610.3390/microorganisms13010016Can Gut Microbiota Analysis Reveal <i>Clostridioides difficile</i> Infection? Evidence from an Italian Cohort at Disease OnsetRoberto Rosato0Gianluca Quaranta1Giulia Santarelli2Giovanni Fancello3Delia Mercedes Bianco4Francesca Romana Monzo5Stefano Bibbò6Giovanni Cammarota7Maurizio Sanguinetti8Luca Masucci9Flavio De Maio10Department of Basic Biotechnological Sciences, Intensivology and Perioperative Clinics, Section of Microbiology, Università Cattolica del Sacro Cuore, 00168 Rome, ItalyDepartment of Laboratory and Infectious Sciences, Fondazione Policlinico Universitario “A. Gemelli” IRCCS, 00168 Rome, ItalyDepartment of Basic Biotechnological Sciences, Intensivology and Perioperative Clinics, Section of Microbiology, Università Cattolica del Sacro Cuore, 00168 Rome, ItalyDepartment of Laboratory and Infectious Sciences, Fondazione Policlinico Universitario “A. Gemelli” IRCCS, 00168 Rome, ItalyHull University Teaching Hospitals NHS Trust, Hull HU3 2JZ, UKDepartment of Laboratory and Infectious Sciences, Fondazione Policlinico Universitario “A. Gemelli” IRCCS, 00168 Rome, ItalyDepartment of Translational Medicine and Surgery, Università Cattolica del Sacro Cuore, 00168 Rome, ItalyDepartment of Translational Medicine and Surgery, Università Cattolica del Sacro Cuore, 00168 Rome, ItalyDepartment of Basic Biotechnological Sciences, Intensivology and Perioperative Clinics, Section of Microbiology, Università Cattolica del Sacro Cuore, 00168 Rome, ItalyDepartment of Basic Biotechnological Sciences, Intensivology and Perioperative Clinics, Section of Microbiology, Università Cattolica del Sacro Cuore, 00168 Rome, ItalyDepartment of Laboratory and Infectious Sciences, Fondazione Policlinico Universitario “A. Gemelli” IRCCS, 00168 Rome, ItalyA diverse and well-functioning gut microbiota normally serves as a protective shield against the invasion of harmful bacteria or the proliferation of opportunistic pathogens. <i>Clostridioides difficile</i> infection (CDI) is predominantly associated with the overuse of antibiotics, resulting in a significant alteration in the gut’s microbial balance. Unfortunately, the lack of global standardization does not allow for the identification of a set of biomarkers associated with the onset and progression of this disease. In this study, we examined the composition of the gut microbiota in patients at the time of the initial detection of CDI compared to a control group of CDI-negative individuals, with a focus on identifying potential CDI biomarkers for diagnosis. While no significant differences in the alpha and beta diversity between CDI-negative and CDI-positive individuals were found, we found that certain genera (such as <i>Clostridium</i> XIVa and <i>Clostridium</i> XVIII) showed different abundance patterns in the two groups, indicating potential differences in gut microbiota balance. In conclusion, am enrichment in <i>Clostridium</i> XI and a decrease in <i>Faecalibacterium</i> emerged in the CDI-positive patients and following antibiotic treatment, indicating that changes in the <i>Clostridium/Faecalibacterium</i> ratio may be a promising biomarker that warrants further investigation for CDI diagnosis.https://www.mdpi.com/2076-2607/13/1/16<i>Clostridioides difficile</i>CDI infectiongut microbiota |
| spellingShingle | Roberto Rosato Gianluca Quaranta Giulia Santarelli Giovanni Fancello Delia Mercedes Bianco Francesca Romana Monzo Stefano Bibbò Giovanni Cammarota Maurizio Sanguinetti Luca Masucci Flavio De Maio Can Gut Microbiota Analysis Reveal <i>Clostridioides difficile</i> Infection? Evidence from an Italian Cohort at Disease Onset Microorganisms <i>Clostridioides difficile</i> CDI infection gut microbiota |
| title | Can Gut Microbiota Analysis Reveal <i>Clostridioides difficile</i> Infection? Evidence from an Italian Cohort at Disease Onset |
| title_full | Can Gut Microbiota Analysis Reveal <i>Clostridioides difficile</i> Infection? Evidence from an Italian Cohort at Disease Onset |
| title_fullStr | Can Gut Microbiota Analysis Reveal <i>Clostridioides difficile</i> Infection? Evidence from an Italian Cohort at Disease Onset |
| title_full_unstemmed | Can Gut Microbiota Analysis Reveal <i>Clostridioides difficile</i> Infection? Evidence from an Italian Cohort at Disease Onset |
| title_short | Can Gut Microbiota Analysis Reveal <i>Clostridioides difficile</i> Infection? Evidence from an Italian Cohort at Disease Onset |
| title_sort | can gut microbiota analysis reveal i clostridioides difficile i infection evidence from an italian cohort at disease onset |
| topic | <i>Clostridioides difficile</i> CDI infection gut microbiota |
| url | https://www.mdpi.com/2076-2607/13/1/16 |
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