Pre-Conditioning with CDP-Choline Attenuates Oxidative Stress-Induced Cardiac Myocyte Death in a Hypoxia/Reperfusion Model
Background. CDP-choline is a key intermediate in the biosynthesis of phosphatidylcholine, which is an essential component of cellular membranes, and a cell signalling mediator. CDP-choline has been used for the treatment of cerebral ischaemia, showing beneficial effects. However, its potential benef...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/187071 |
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| author | Héctor González-Pacheco Aurelio Méndez-Domínguez Salomón Hernández Rebeca López-Marure Maria J. Vazquez-Mellado Cecilia Aguilar Leticia Rocha-Zavaleta |
| author_facet | Héctor González-Pacheco Aurelio Méndez-Domínguez Salomón Hernández Rebeca López-Marure Maria J. Vazquez-Mellado Cecilia Aguilar Leticia Rocha-Zavaleta |
| author_sort | Héctor González-Pacheco |
| collection | DOAJ |
| description | Background. CDP-choline is a key intermediate in the biosynthesis of phosphatidylcholine, which is an essential component of cellular membranes, and a cell signalling mediator. CDP-choline has been used for the treatment of cerebral ischaemia, showing beneficial effects. However, its potential benefit for the treatment of myocardial ischaemia has not been explored yet. Aim. In the present work, we aimed to evaluate the potential use of CDP-choline as a cardioprotector in an in vitro model of ischaemia/reperfusion injury. Methods. Neonatal rat cardiac myocytes were isolated and subjected to hypoxia/reperfusion using the coverslip hypoxia model. To evaluate the effect of CDP-choline on oxidative stress-induced reperfusion injury, the cells were incubated with H2O2 during reperfusion. The effect of CDP-choline pre- and postconditioning was evaluated using the cell viability MTT assay, and the proportion of apoptotic and necrotic cells was analyzed using the Annexin V determination by flow cytometry. Results. Pre- and postconditioning with 50 mg/mL of CDP-choline induced a significant reduction of cells undergoing apoptosis after hypoxia/reperfusion. Preconditioning with CDP-choline attenuated postreperfusion cell death induced by oxidative stress. Conclusion. CDP-choline administration reduces cell apoptosis induced by oxidative stress after hypoxia/reperfusion of cardiac myocytes. Thus, it has a potential as cardioprotector in ischaemia/reperfusion-injured cardiomyocytes. |
| format | Article |
| id | doaj-art-d6c3edd0ffc4426583f73d51611ce8ba |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-d6c3edd0ffc4426583f73d51611ce8ba2025-02-03T01:28:05ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/187071187071Pre-Conditioning with CDP-Choline Attenuates Oxidative Stress-Induced Cardiac Myocyte Death in a Hypoxia/Reperfusion ModelHéctor González-Pacheco0Aurelio Méndez-Domínguez1Salomón Hernández2Rebeca López-Marure3Maria J. Vazquez-Mellado4Cecilia Aguilar5Leticia Rocha-Zavaleta6Departamento de Urgencias y Unidad Coronaria, Instituto Nacional de Cardiología “Ignacio Chavez”, Juan Badiano No. 1, Colonia Seccion 16, 14080 Tlalpan, DF, MexicoDepartamento de Neurologia, Instituto Nacional de Cardiología “Ignacio Chavez”, Juan Badiano No. 1, Colonia Seccion 16, 14080 Tlalpan, DF, MexicoFacultad de Medicina, Universidad Panamericana, Augusto Rodin 498, 03920 Insurgentes Mixcoac, DF, MexicoDepartamento de Biologia Celular, Instituto Nacional de Cardiología “Ignacio Chavez”, Juan Badiano No. 1, Colonia Seccion 16, 14080 Tlalpan, DF, MexicoDepartamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, UNAM, Circuito Escolar s/n, Ciudad Universitaria, 04510 Coyoacan, DF, MexicoDepartamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, UNAM, Circuito Escolar s/n, Ciudad Universitaria, 04510 Coyoacan, DF, MexicoDepartamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, UNAM, Circuito Escolar s/n, Ciudad Universitaria, 04510 Coyoacan, DF, MexicoBackground. CDP-choline is a key intermediate in the biosynthesis of phosphatidylcholine, which is an essential component of cellular membranes, and a cell signalling mediator. CDP-choline has been used for the treatment of cerebral ischaemia, showing beneficial effects. However, its potential benefit for the treatment of myocardial ischaemia has not been explored yet. Aim. In the present work, we aimed to evaluate the potential use of CDP-choline as a cardioprotector in an in vitro model of ischaemia/reperfusion injury. Methods. Neonatal rat cardiac myocytes were isolated and subjected to hypoxia/reperfusion using the coverslip hypoxia model. To evaluate the effect of CDP-choline on oxidative stress-induced reperfusion injury, the cells were incubated with H2O2 during reperfusion. The effect of CDP-choline pre- and postconditioning was evaluated using the cell viability MTT assay, and the proportion of apoptotic and necrotic cells was analyzed using the Annexin V determination by flow cytometry. Results. Pre- and postconditioning with 50 mg/mL of CDP-choline induced a significant reduction of cells undergoing apoptosis after hypoxia/reperfusion. Preconditioning with CDP-choline attenuated postreperfusion cell death induced by oxidative stress. Conclusion. CDP-choline administration reduces cell apoptosis induced by oxidative stress after hypoxia/reperfusion of cardiac myocytes. Thus, it has a potential as cardioprotector in ischaemia/reperfusion-injured cardiomyocytes.http://dx.doi.org/10.1155/2014/187071 |
| spellingShingle | Héctor González-Pacheco Aurelio Méndez-Domínguez Salomón Hernández Rebeca López-Marure Maria J. Vazquez-Mellado Cecilia Aguilar Leticia Rocha-Zavaleta Pre-Conditioning with CDP-Choline Attenuates Oxidative Stress-Induced Cardiac Myocyte Death in a Hypoxia/Reperfusion Model The Scientific World Journal |
| title | Pre-Conditioning with CDP-Choline Attenuates Oxidative Stress-Induced Cardiac Myocyte Death in a Hypoxia/Reperfusion Model |
| title_full | Pre-Conditioning with CDP-Choline Attenuates Oxidative Stress-Induced Cardiac Myocyte Death in a Hypoxia/Reperfusion Model |
| title_fullStr | Pre-Conditioning with CDP-Choline Attenuates Oxidative Stress-Induced Cardiac Myocyte Death in a Hypoxia/Reperfusion Model |
| title_full_unstemmed | Pre-Conditioning with CDP-Choline Attenuates Oxidative Stress-Induced Cardiac Myocyte Death in a Hypoxia/Reperfusion Model |
| title_short | Pre-Conditioning with CDP-Choline Attenuates Oxidative Stress-Induced Cardiac Myocyte Death in a Hypoxia/Reperfusion Model |
| title_sort | pre conditioning with cdp choline attenuates oxidative stress induced cardiac myocyte death in a hypoxia reperfusion model |
| url | http://dx.doi.org/10.1155/2014/187071 |
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