Biological Control of <i>Streptomyces</i> sp. PR69 Against <i>Phytophthora capsici</i> and Its Growth-Promoting Effects on Plants
Actinomycetes are a group of bacteria that have been reported as potential controllers of several plant pathogens and plant growth-promoting agents. This study focused on the isolation of 60 actinobacterial strains from the Cuatro Cienegas Basin, Coahuila, Mexico, with an emphasis on evaluating thei...
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2024-12-01
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| author | Perla Karina López-Reyes Susana De la Torre-Zavala María Mercedes Cortés-González Luis Jesús Galán-Wong Hamlet Avilés-Arnaut |
| author_facet | Perla Karina López-Reyes Susana De la Torre-Zavala María Mercedes Cortés-González Luis Jesús Galán-Wong Hamlet Avilés-Arnaut |
| author_sort | Perla Karina López-Reyes |
| collection | DOAJ |
| description | Actinomycetes are a group of bacteria that have been reported as potential controllers of several plant pathogens and plant growth-promoting agents. This study focused on the isolation of 60 actinobacterial strains from the Cuatro Cienegas Basin, Coahuila, Mexico, with an emphasis on evaluating their potential as biocontrol agents against plant pathogens. Among the strains tested, the isolate PR69 exhibited significant in vitro antagonistic activity against eight plant pathogens, with inhibition rates ranging from 44% to 73%, including <i>Phytophthora capsici</i>. The genome of PR69 was sequenced and assembled. It was identified as <i>Streptomyces</i> by phylogenetic analysis based on concatenated multiple sequence alignments of 81 core bacterial genes. Additionally, volatile compounds produced by PR69 enhanced the growth of <i>Arabidopsis thaliana</i> seedlings in vitro, increasing seedling weight, primary root length, and the number of secondary roots. Furthermore, the soil treated with <i>Streptomyces</i> sp. PR69 effectively controlled the infection caused by the pathogen <i>P. capsici</i> in bell pepper plants, reducing mortality by 47% compared to plants inoculated solely with the pathogen. PR69-treated plants also showed 30% increase in fresh weight compared to untreated controls. These findings suggest that <i>Streptomyces</i> sp. PR69 holds promise as a bioinoculant for promoting pepper plant growth and controlling <i>P. capsici</i> populations. |
| format | Article |
| id | doaj-art-d688fe1440ef46d4b51a7c3d9d07d088 |
| institution | DOAJ |
| issn | 2311-7524 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Horticulturae |
| spelling | doaj-art-d688fe1440ef46d4b51a7c3d9d07d0882025-08-20T02:55:38ZengMDPI AGHorticulturae2311-75242024-12-011012136510.3390/horticulturae10121365Biological Control of <i>Streptomyces</i> sp. PR69 Against <i>Phytophthora capsici</i> and Its Growth-Promoting Effects on PlantsPerla Karina López-Reyes0Susana De la Torre-Zavala1María Mercedes Cortés-González2Luis Jesús Galán-Wong3Hamlet Avilés-Arnaut4Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Instituto de Biotecnología, Avenida Universidad S/N, Ciudad Universitaria, San Nicolás de los Garza CP 66455, Nuevo León, MexicoUniversidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Instituto de Biotecnología, Avenida Universidad S/N, Ciudad Universitaria, San Nicolás de los Garza CP 66455, Nuevo León, MexicoUniversidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Instituto de Biotecnología, Avenida Universidad S/N, Ciudad Universitaria, San Nicolás de los Garza CP 66455, Nuevo León, MexicoUniversidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Instituto de Biotecnología, Avenida Universidad S/N, Ciudad Universitaria, San Nicolás de los Garza CP 66455, Nuevo León, MexicoUniversidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Instituto de Biotecnología, Avenida Universidad S/N, Ciudad Universitaria, San Nicolás de los Garza CP 66455, Nuevo León, MexicoActinomycetes are a group of bacteria that have been reported as potential controllers of several plant pathogens and plant growth-promoting agents. This study focused on the isolation of 60 actinobacterial strains from the Cuatro Cienegas Basin, Coahuila, Mexico, with an emphasis on evaluating their potential as biocontrol agents against plant pathogens. Among the strains tested, the isolate PR69 exhibited significant in vitro antagonistic activity against eight plant pathogens, with inhibition rates ranging from 44% to 73%, including <i>Phytophthora capsici</i>. The genome of PR69 was sequenced and assembled. It was identified as <i>Streptomyces</i> by phylogenetic analysis based on concatenated multiple sequence alignments of 81 core bacterial genes. Additionally, volatile compounds produced by PR69 enhanced the growth of <i>Arabidopsis thaliana</i> seedlings in vitro, increasing seedling weight, primary root length, and the number of secondary roots. Furthermore, the soil treated with <i>Streptomyces</i> sp. PR69 effectively controlled the infection caused by the pathogen <i>P. capsici</i> in bell pepper plants, reducing mortality by 47% compared to plants inoculated solely with the pathogen. PR69-treated plants also showed 30% increase in fresh weight compared to untreated controls. These findings suggest that <i>Streptomyces</i> sp. PR69 holds promise as a bioinoculant for promoting pepper plant growth and controlling <i>P. capsici</i> populations.https://www.mdpi.com/2311-7524/10/12/1365antifungal activityphytopathogenbiocontrol<i>Streptomyces</i><i>Capsicum annum</i>plant growth promotion |
| spellingShingle | Perla Karina López-Reyes Susana De la Torre-Zavala María Mercedes Cortés-González Luis Jesús Galán-Wong Hamlet Avilés-Arnaut Biological Control of <i>Streptomyces</i> sp. PR69 Against <i>Phytophthora capsici</i> and Its Growth-Promoting Effects on Plants Horticulturae antifungal activity phytopathogen biocontrol <i>Streptomyces</i> <i>Capsicum annum</i> plant growth promotion |
| title | Biological Control of <i>Streptomyces</i> sp. PR69 Against <i>Phytophthora capsici</i> and Its Growth-Promoting Effects on Plants |
| title_full | Biological Control of <i>Streptomyces</i> sp. PR69 Against <i>Phytophthora capsici</i> and Its Growth-Promoting Effects on Plants |
| title_fullStr | Biological Control of <i>Streptomyces</i> sp. PR69 Against <i>Phytophthora capsici</i> and Its Growth-Promoting Effects on Plants |
| title_full_unstemmed | Biological Control of <i>Streptomyces</i> sp. PR69 Against <i>Phytophthora capsici</i> and Its Growth-Promoting Effects on Plants |
| title_short | Biological Control of <i>Streptomyces</i> sp. PR69 Against <i>Phytophthora capsici</i> and Its Growth-Promoting Effects on Plants |
| title_sort | biological control of i streptomyces i sp pr69 against i phytophthora capsici i and its growth promoting effects on plants |
| topic | antifungal activity phytopathogen biocontrol <i>Streptomyces</i> <i>Capsicum annum</i> plant growth promotion |
| url | https://www.mdpi.com/2311-7524/10/12/1365 |
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