Multiple pathways to evaluate the immunoprotective effect of Turkeys Herpesvirus recombinant vaccine expressing HA of H9N2
ABSTRACT: H9N2 avian influenza virus is a significant poultry pathogen that provides internal genes for multiple zoonotic subtypes of avian influenza, presenting a severe threat to public health. The isolation rate of H9N2 in poultry has increased annually in recent years. In this study, a recombina...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124009143 |
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| author | Wenhao Yang Jin Zhang Jing Dai Mengjiao Guo Xiaolong Lu Ruyi Gao Kaituo Liu Min Gu Shunlin Hu Xiufan Liu Xiaoquan Wang Xiaowen Liu |
| author_facet | Wenhao Yang Jin Zhang Jing Dai Mengjiao Guo Xiaolong Lu Ruyi Gao Kaituo Liu Min Gu Shunlin Hu Xiufan Liu Xiaoquan Wang Xiaowen Liu |
| author_sort | Wenhao Yang |
| collection | DOAJ |
| description | ABSTRACT: H9N2 avian influenza virus is a significant poultry pathogen that provides internal genes for multiple zoonotic subtypes of avian influenza, presenting a severe threat to public health. The isolation rate of H9N2 in poultry has increased annually in recent years. In this study, a recombinant Herpesvirus of Turkeys (HVT) vaccine expressing H9-HA was constructed using Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology. In the construction of HVT-EGFP-HA recombinant virus, nonhomologous end joining (NHEJ) is a much more efficient strategy compare to Homology-directed recombination (HDR). HVT-HA demonstrated stability and consistent replication with the parent strain. Subcutaneous injection and in-ovo injection of HVT-HA induced different levels of immune response. Compared to in-ovo injection of HVT-HA, subcutaneous injection induced significantly higher neutralizing serum antibodies. This finding is supported by the significantly higher CD4+ T cell response in Peripheral blood mononuclear cell Peripheral blood mononuclear cell (PBMC) in the subcutaneous injection group. However, in-ovo injection of HVT-HA resulted in significantly higher neutralizing antibodies in the Harderian glands. In addition, it significantly inhibited viral shedding after intranasal exposure to H9N2. This phenomenon could be attributed to the mucosal immunity present in the Hadrian gland. Thus, our findings indicate that the in-ovo injection of the HVT-HA recombinant vaccine is a promising method to inhibit the transmission of H9N2 via the upper respiratory tract in chickens. |
| format | Article |
| id | doaj-art-d67607f7462a4599b59a79d73652d646 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-d67607f7462a4599b59a79d73652d6462025-01-22T05:40:07ZengElsevierPoultry Science0032-57912025-01-011041104335Multiple pathways to evaluate the immunoprotective effect of Turkeys Herpesvirus recombinant vaccine expressing HA of H9N2Wenhao Yang0Jin Zhang1Jing Dai2Mengjiao Guo3Xiaolong Lu4Ruyi Gao5Kaituo Liu6Min Gu7Shunlin Hu8Xiufan Liu9Xiaoquan Wang10Xiaowen Liu11Key Laboratory of Avian Bioproducts Development, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, ChinaKey Laboratory of Avian Bioproducts Development, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, ChinaKey Laboratory of Avian Bioproducts Development, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, ChinaKey Laboratory of Avian Bioproducts Development, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, ChinaKey Laboratory of Avian Bioproducts Development, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou 225009, Jiangsu, China; Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Avian Bioproducts Development, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou 225009, Jiangsu, China; Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, ChinaJiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou 225009, Jiangsu, China; Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Avian Bioproducts Development, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou 225009, Jiangsu, China; Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Avian Bioproducts Development, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou 225009, Jiangsu, China; Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Avian Bioproducts Development, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou 225009, Jiangsu, China; Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, ChinaKey Laboratory of Avian Bioproducts Development, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou 225009, Jiangsu, China; Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China; Corresponding author:Key Laboratory of Avian Bioproducts Development, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, Jiangsu, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou University, Yangzhou 225009, Jiangsu, China; Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China; Corresponding author:ABSTRACT: H9N2 avian influenza virus is a significant poultry pathogen that provides internal genes for multiple zoonotic subtypes of avian influenza, presenting a severe threat to public health. The isolation rate of H9N2 in poultry has increased annually in recent years. In this study, a recombinant Herpesvirus of Turkeys (HVT) vaccine expressing H9-HA was constructed using Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology. In the construction of HVT-EGFP-HA recombinant virus, nonhomologous end joining (NHEJ) is a much more efficient strategy compare to Homology-directed recombination (HDR). HVT-HA demonstrated stability and consistent replication with the parent strain. Subcutaneous injection and in-ovo injection of HVT-HA induced different levels of immune response. Compared to in-ovo injection of HVT-HA, subcutaneous injection induced significantly higher neutralizing serum antibodies. This finding is supported by the significantly higher CD4+ T cell response in Peripheral blood mononuclear cell Peripheral blood mononuclear cell (PBMC) in the subcutaneous injection group. However, in-ovo injection of HVT-HA resulted in significantly higher neutralizing antibodies in the Harderian glands. In addition, it significantly inhibited viral shedding after intranasal exposure to H9N2. This phenomenon could be attributed to the mucosal immunity present in the Hadrian gland. Thus, our findings indicate that the in-ovo injection of the HVT-HA recombinant vaccine is a promising method to inhibit the transmission of H9N2 via the upper respiratory tract in chickens.http://www.sciencedirect.com/science/article/pii/S0032579124009143H9N2vector vaccineherpesvirus of Turkeychicken |
| spellingShingle | Wenhao Yang Jin Zhang Jing Dai Mengjiao Guo Xiaolong Lu Ruyi Gao Kaituo Liu Min Gu Shunlin Hu Xiufan Liu Xiaoquan Wang Xiaowen Liu Multiple pathways to evaluate the immunoprotective effect of Turkeys Herpesvirus recombinant vaccine expressing HA of H9N2 Poultry Science H9N2 vector vaccine herpesvirus of Turkey chicken |
| title | Multiple pathways to evaluate the immunoprotective effect of Turkeys Herpesvirus recombinant vaccine expressing HA of H9N2 |
| title_full | Multiple pathways to evaluate the immunoprotective effect of Turkeys Herpesvirus recombinant vaccine expressing HA of H9N2 |
| title_fullStr | Multiple pathways to evaluate the immunoprotective effect of Turkeys Herpesvirus recombinant vaccine expressing HA of H9N2 |
| title_full_unstemmed | Multiple pathways to evaluate the immunoprotective effect of Turkeys Herpesvirus recombinant vaccine expressing HA of H9N2 |
| title_short | Multiple pathways to evaluate the immunoprotective effect of Turkeys Herpesvirus recombinant vaccine expressing HA of H9N2 |
| title_sort | multiple pathways to evaluate the immunoprotective effect of turkeys herpesvirus recombinant vaccine expressing ha of h9n2 |
| topic | H9N2 vector vaccine herpesvirus of Turkey chicken |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124009143 |
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