NGS sequencing in barley breeding and genetic studies
Barley (Hordeum vulgare L.) is the one of the most important cereal species used as food and feed crops, as well as for malting and alcohol production. At the end of the last century, traditional breeding techniques were complemented by the use of DNA markers. Molecular markers have also been used e...
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| Format: | Article |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2020-07-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/2643 |
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| author | I. V. Rozanova E. K. Khlestkina |
| author_facet | I. V. Rozanova E. K. Khlestkina |
| author_sort | I. V. Rozanova |
| collection | DOAJ |
| description | Barley (Hordeum vulgare L.) is the one of the most important cereal species used as food and feed crops, as well as for malting and alcohol production. At the end of the last century, traditional breeding techniques were complemented by the use of DNA markers. Molecular markers have also been used extensively for molecular genetic mapping and QTL analysis. In 2012, the barley genome sequencing was completed, which provided a broad range of new opportunities – from a more efficient search for candidate genes controlling economically important traits to genomic selection. The review summarizes the results of the studies performed after barley genome sequencing, which discovered new areas of barley genetics and breeding with high throughput screening and genotyping methods. During this period, intensive studies aimed at identification of barley genomic loci associated with economically important traits have been carried out; online databases and tools for working with barley genomic data and their deposition have appeared and are being replenished. In recent years, GWAS analysis has been used for large-scale phenotypegenotype association studies, which has been widely used in barley since 2010 due to the developed SNP-arrays, as well as genotyping methods based on direct NGS sequencing of selected fractions of the genome. To date, more than 80 papers have been published that describe the results of the GWAS analysis in barley. SNP identification associated with economically important traits and their transformation into CAPS or KASP markers convenient for screening selection material significantly expands the possibilities of marker-assisted selection of barley. In addition, the currently available information on potential target genes and the quality of the whole barley genome sequence provides a good base for applying genome editing technologies to create material for the creation of varieties with desired properties. |
| format | Article |
| id | doaj-art-d671bac3ec2640cebb698a3c76ab6432 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2020-07-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-d671bac3ec2640cebb698a3c76ab64322025-02-01T09:58:09ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592020-07-0124434835510.18699/VJ20.6271059NGS sequencing in barley breeding and genetic studiesI. V. Rozanova0E. K. Khlestkina1Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR); Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of SciencesFederal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR); Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of SciencesBarley (Hordeum vulgare L.) is the one of the most important cereal species used as food and feed crops, as well as for malting and alcohol production. At the end of the last century, traditional breeding techniques were complemented by the use of DNA markers. Molecular markers have also been used extensively for molecular genetic mapping and QTL analysis. In 2012, the barley genome sequencing was completed, which provided a broad range of new opportunities – from a more efficient search for candidate genes controlling economically important traits to genomic selection. The review summarizes the results of the studies performed after barley genome sequencing, which discovered new areas of barley genetics and breeding with high throughput screening and genotyping methods. During this period, intensive studies aimed at identification of barley genomic loci associated with economically important traits have been carried out; online databases and tools for working with barley genomic data and their deposition have appeared and are being replenished. In recent years, GWAS analysis has been used for large-scale phenotypegenotype association studies, which has been widely used in barley since 2010 due to the developed SNP-arrays, as well as genotyping methods based on direct NGS sequencing of selected fractions of the genome. To date, more than 80 papers have been published that describe the results of the GWAS analysis in barley. SNP identification associated with economically important traits and their transformation into CAPS or KASP markers convenient for screening selection material significantly expands the possibilities of marker-assisted selection of barley. In addition, the currently available information on potential target genes and the quality of the whole barley genome sequence provides a good base for applying genome editing technologies to create material for the creation of varieties with desired properties.https://vavilov.elpub.ru/jour/article/view/2643<i>hordeum vulgare</i>snpbarleygenomesequencingthroughput genotypinggenomic editing |
| spellingShingle | I. V. Rozanova E. K. Khlestkina NGS sequencing in barley breeding and genetic studies Вавиловский журнал генетики и селекции <i>hordeum vulgare</i> snp barley genome sequencing throughput genotyping genomic editing |
| title | NGS sequencing in barley breeding and genetic studies |
| title_full | NGS sequencing in barley breeding and genetic studies |
| title_fullStr | NGS sequencing in barley breeding and genetic studies |
| title_full_unstemmed | NGS sequencing in barley breeding and genetic studies |
| title_short | NGS sequencing in barley breeding and genetic studies |
| title_sort | ngs sequencing in barley breeding and genetic studies |
| topic | <i>hordeum vulgare</i> snp barley genome sequencing throughput genotyping genomic editing |
| url | https://vavilov.elpub.ru/jour/article/view/2643 |
| work_keys_str_mv | AT ivrozanova ngssequencinginbarleybreedingandgeneticstudies AT ekkhlestkina ngssequencinginbarleybreedingandgeneticstudies |