Real-Time Multichannel Imaging Framework for Endoscopy, Catheters, and Fixed Geometry Intraoperative Systems
To address the need for a clinically applicable intravital optical imaging system, we developed a new hardware and software framework. We demonstrate its utility by applying it to an endoscope-based white light and fluorescent imaging system. The capabilities include acquisition and visualization al...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2007-05-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2007.00012 |
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| _version_ | 1832544646326124544 |
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| author | Rahul A. Sheth Rabi Upadhyay Ralph Weissleder Umar Mahmood |
| author_facet | Rahul A. Sheth Rabi Upadhyay Ralph Weissleder Umar Mahmood |
| author_sort | Rahul A. Sheth |
| collection | DOAJ |
| description | To address the need for a clinically applicable intravital optical imaging system, we developed a new hardware and software framework. We demonstrate its utility by applying it to an endoscope-based white light and fluorescent imaging system. The capabilities include acquisition and visualization algorithms that perform registration, segmentation, and histogram-based autoexposure of two imaging channels (full-spectrum white light and near-infrared fluorescence), all in real time. Data are processed and saved as 12-bit files, matching the standards of clinical imaging. Dynamic range is further improved by the evaluation of flux as a quantitative parameter. The above features are demonstrated in a series of in vitro experiments, and the in vivo application is shown with the visualization of fluorescent-labeled vasculature of a mouse peritoneum. The approach may be applied to diverse systems, including handheld devices, fixed geometry intraoperative devices, catheter-based imaging, and multimodal systems. |
| format | Article |
| id | doaj-art-d64cc28e910843bdb9c2794296a5a613 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2007-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-d64cc28e910843bdb9c2794296a5a6132025-02-03T10:07:58ZengSAGE PublishingMolecular Imaging1536-01212007-05-01610.2310/7290.2007.0001210.2310_7290.2007.00012Real-Time Multichannel Imaging Framework for Endoscopy, Catheters, and Fixed Geometry Intraoperative SystemsRahul A. ShethRabi UpadhyayRalph WeisslederUmar MahmoodTo address the need for a clinically applicable intravital optical imaging system, we developed a new hardware and software framework. We demonstrate its utility by applying it to an endoscope-based white light and fluorescent imaging system. The capabilities include acquisition and visualization algorithms that perform registration, segmentation, and histogram-based autoexposure of two imaging channels (full-spectrum white light and near-infrared fluorescence), all in real time. Data are processed and saved as 12-bit files, matching the standards of clinical imaging. Dynamic range is further improved by the evaluation of flux as a quantitative parameter. The above features are demonstrated in a series of in vitro experiments, and the in vivo application is shown with the visualization of fluorescent-labeled vasculature of a mouse peritoneum. The approach may be applied to diverse systems, including handheld devices, fixed geometry intraoperative devices, catheter-based imaging, and multimodal systems.https://doi.org/10.2310/7290.2007.00012 |
| spellingShingle | Rahul A. Sheth Rabi Upadhyay Ralph Weissleder Umar Mahmood Real-Time Multichannel Imaging Framework for Endoscopy, Catheters, and Fixed Geometry Intraoperative Systems Molecular Imaging |
| title | Real-Time Multichannel Imaging Framework for Endoscopy, Catheters, and Fixed Geometry Intraoperative Systems |
| title_full | Real-Time Multichannel Imaging Framework for Endoscopy, Catheters, and Fixed Geometry Intraoperative Systems |
| title_fullStr | Real-Time Multichannel Imaging Framework for Endoscopy, Catheters, and Fixed Geometry Intraoperative Systems |
| title_full_unstemmed | Real-Time Multichannel Imaging Framework for Endoscopy, Catheters, and Fixed Geometry Intraoperative Systems |
| title_short | Real-Time Multichannel Imaging Framework for Endoscopy, Catheters, and Fixed Geometry Intraoperative Systems |
| title_sort | real time multichannel imaging framework for endoscopy catheters and fixed geometry intraoperative systems |
| url | https://doi.org/10.2310/7290.2007.00012 |
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