Identification of Molecular Signatures in Neural Differentiation and Neurological Diseases Using Digital Color-Coded Molecular Barcoding
Human pluripotent stem cells (PSCs), including embryonic stem cells and induced pluripotent stem cells, represent powerful tools for disease modeling and for therapeutic applications. PSCs are particularly useful for the study of development and diseases of the nervous system. However, generating in...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2020/8852313 |
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| _version_ | 1832546975599296512 |
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| author | Debora Salerno Alessandro Rosa |
| author_facet | Debora Salerno Alessandro Rosa |
| author_sort | Debora Salerno |
| collection | DOAJ |
| description | Human pluripotent stem cells (PSCs), including embryonic stem cells and induced pluripotent stem cells, represent powerful tools for disease modeling and for therapeutic applications. PSCs are particularly useful for the study of development and diseases of the nervous system. However, generating in vitro models that recapitulate the architecture and the full variety of subtypes of cells that make the complexity of our brain remains a challenge. In order to fully exploit the potential of PSCs, advanced methods that facilitate the identification of molecular signatures in neural differentiation and neurological diseases are highly demanded. Here, we review the literature on the development and application of digital color-coded molecular barcoding as a potential tool for standardizing PSC research and applications in neuroscience. We will also describe relevant examples of the use of this technique for the characterization of the heterogeneous composition of the brain tumor glioblastoma multiforme. |
| format | Article |
| id | doaj-art-d641f367bfc1432291acda932953e87d |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-d641f367bfc1432291acda932953e87d2025-02-03T06:46:28ZengWileyStem Cells International1687-966X1687-96782020-01-01202010.1155/2020/88523138852313Identification of Molecular Signatures in Neural Differentiation and Neurological Diseases Using Digital Color-Coded Molecular BarcodingDebora Salerno0Alessandro Rosa1Center for Life Nano Science, Istituto Italiano di Tecnologia, Rome 00161, ItalyCenter for Life Nano Science, Istituto Italiano di Tecnologia, Rome 00161, ItalyHuman pluripotent stem cells (PSCs), including embryonic stem cells and induced pluripotent stem cells, represent powerful tools for disease modeling and for therapeutic applications. PSCs are particularly useful for the study of development and diseases of the nervous system. However, generating in vitro models that recapitulate the architecture and the full variety of subtypes of cells that make the complexity of our brain remains a challenge. In order to fully exploit the potential of PSCs, advanced methods that facilitate the identification of molecular signatures in neural differentiation and neurological diseases are highly demanded. Here, we review the literature on the development and application of digital color-coded molecular barcoding as a potential tool for standardizing PSC research and applications in neuroscience. We will also describe relevant examples of the use of this technique for the characterization of the heterogeneous composition of the brain tumor glioblastoma multiforme.http://dx.doi.org/10.1155/2020/8852313 |
| spellingShingle | Debora Salerno Alessandro Rosa Identification of Molecular Signatures in Neural Differentiation and Neurological Diseases Using Digital Color-Coded Molecular Barcoding Stem Cells International |
| title | Identification of Molecular Signatures in Neural Differentiation and Neurological Diseases Using Digital Color-Coded Molecular Barcoding |
| title_full | Identification of Molecular Signatures in Neural Differentiation and Neurological Diseases Using Digital Color-Coded Molecular Barcoding |
| title_fullStr | Identification of Molecular Signatures in Neural Differentiation and Neurological Diseases Using Digital Color-Coded Molecular Barcoding |
| title_full_unstemmed | Identification of Molecular Signatures in Neural Differentiation and Neurological Diseases Using Digital Color-Coded Molecular Barcoding |
| title_short | Identification of Molecular Signatures in Neural Differentiation and Neurological Diseases Using Digital Color-Coded Molecular Barcoding |
| title_sort | identification of molecular signatures in neural differentiation and neurological diseases using digital color coded molecular barcoding |
| url | http://dx.doi.org/10.1155/2020/8852313 |
| work_keys_str_mv | AT deborasalerno identificationofmolecularsignaturesinneuraldifferentiationandneurologicaldiseasesusingdigitalcolorcodedmolecularbarcoding AT alessandrorosa identificationofmolecularsignaturesinneuraldifferentiationandneurologicaldiseasesusingdigitalcolorcodedmolecularbarcoding |