Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells
As stromal cells and recently confirmed mesenchymal stem cells, OP9 cells support hematopoiesis stem cell (HSC) differentiation into the B lymphocyte lineage, yet Delta-like-1 (DL1) overexpressing OP9 (OP9DL1) cells promote the development of early T lymphocytes from HSC. However, the immunomodulato...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/1628352 |
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| author | Lei Zhang Rui-Jie Dang Yan-Mei Yang Dian-Chao Cui Ping Li Yan-Li Ni Tong Hao Changyong Wang Xiao-Xia Jiang Nan-Zhu Fang |
| author_facet | Lei Zhang Rui-Jie Dang Yan-Mei Yang Dian-Chao Cui Ping Li Yan-Li Ni Tong Hao Changyong Wang Xiao-Xia Jiang Nan-Zhu Fang |
| author_sort | Lei Zhang |
| collection | DOAJ |
| description | As stromal cells and recently confirmed mesenchymal stem cells, OP9 cells support hematopoiesis stem cell (HSC) differentiation into the B lymphocyte lineage, yet Delta-like-1 (DL1) overexpressing OP9 (OP9DL1) cells promote the development of early T lymphocytes from HSC. However, the immunomodulatory capacity of OP9 or OP9DL1 on mature B and T cell proliferation has not been elucidated. Here, we show that OP9 and OP9DL1 have similar proliferation capacities and immunophenotypes except DL1 expression. Compared with OP9, OP9DL1 displayed more osteogenesis and less adipogenesis when cultured in the respective induction media. Both OP9 and OP9DL1 inhibited mature B and T cell proliferation. Furthermore, OP9 showed stronger inhibition on B cell proliferation and OP9DL1 exhibited stronger inhibition on T cell proliferation. With stimulation, both OP9 and OP9DL1 showed increased nitrate oxide (NO) production. The NO levels of OP9 were higher than that of OP9DL1 when stimulated with TNFα/IFNγ or LPS/IL4. Taken together, our study reveals a previously unrecognized role of OP9 and OP9DL1 in mature B and T cell proliferation. DL1 overexpression alone changed the properties of OP9 cells in addition to their role in early B cell development. |
| format | Article |
| id | doaj-art-d601e51104d74c029e63a85888fc6590 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-d601e51104d74c029e63a85888fc65902025-02-03T06:00:43ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/16283521628352Delta-Like-1 Changes the Immunomodulatory Property of OP9 CellsLei Zhang0Rui-Jie Dang1Yan-Mei Yang2Dian-Chao Cui3Ping Li4Yan-Li Ni5Tong Hao6Changyong Wang7Xiao-Xia Jiang8Nan-Zhu Fang9Laboratory of Animal Genetic Breeding and Reproduction, Yanbian University, Yanji, Jilin 133002, ChinaDepartment of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences, 27 Taiping Road, Haidian District, Beijing 100850, ChinaDepartment of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences, 27 Taiping Road, Haidian District, Beijing 100850, ChinaBeijing Aiyuhua Hospital for Children and Women, 2 South Street, Beijing Economic and Technological Development Zone, Beijing 100176, ChinaDepartment of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences, 27 Taiping Road, Haidian District, Beijing 100850, China307-Ivy Translational Medicine Center, Laboratory of Oncology, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, ChinaDepartment of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences, 27 Taiping Road, Haidian District, Beijing 100850, ChinaDepartment of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences, 27 Taiping Road, Haidian District, Beijing 100850, ChinaDepartment of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences, 27 Taiping Road, Haidian District, Beijing 100850, ChinaLaboratory of Animal Genetic Breeding and Reproduction, Yanbian University, Yanji, Jilin 133002, ChinaAs stromal cells and recently confirmed mesenchymal stem cells, OP9 cells support hematopoiesis stem cell (HSC) differentiation into the B lymphocyte lineage, yet Delta-like-1 (DL1) overexpressing OP9 (OP9DL1) cells promote the development of early T lymphocytes from HSC. However, the immunomodulatory capacity of OP9 or OP9DL1 on mature B and T cell proliferation has not been elucidated. Here, we show that OP9 and OP9DL1 have similar proliferation capacities and immunophenotypes except DL1 expression. Compared with OP9, OP9DL1 displayed more osteogenesis and less adipogenesis when cultured in the respective induction media. Both OP9 and OP9DL1 inhibited mature B and T cell proliferation. Furthermore, OP9 showed stronger inhibition on B cell proliferation and OP9DL1 exhibited stronger inhibition on T cell proliferation. With stimulation, both OP9 and OP9DL1 showed increased nitrate oxide (NO) production. The NO levels of OP9 were higher than that of OP9DL1 when stimulated with TNFα/IFNγ or LPS/IL4. Taken together, our study reveals a previously unrecognized role of OP9 and OP9DL1 in mature B and T cell proliferation. DL1 overexpression alone changed the properties of OP9 cells in addition to their role in early B cell development.http://dx.doi.org/10.1155/2016/1628352 |
| spellingShingle | Lei Zhang Rui-Jie Dang Yan-Mei Yang Dian-Chao Cui Ping Li Yan-Li Ni Tong Hao Changyong Wang Xiao-Xia Jiang Nan-Zhu Fang Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells Stem Cells International |
| title | Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells |
| title_full | Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells |
| title_fullStr | Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells |
| title_full_unstemmed | Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells |
| title_short | Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells |
| title_sort | delta like 1 changes the immunomodulatory property of op9 cells |
| url | http://dx.doi.org/10.1155/2016/1628352 |
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