NuclampFISH enables cell sorting based on nuclear RNA expression for chromatin analysis

NuclampFISH enables cell sorting based on nuclear RNA expression for chromatin analysis

Abstract Background Transcriptional bursts are periods when RNA polymerase interacts with a DNA locus, leading to active gene transcription. This bursting activity can vary across individual cells, and analyzing the differences in transcription sites can help identify key drivers of gene expression...

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Bibliographic Details
Main Authors: Yifang Liu, Yuchen Qiu, Keqing Nian, Sara H. Rouhanifard
Format: Article
Language:English
Published: BMC 2025-07-01
Series:BMC Genomics
Subjects:
SmFISH
RNA FISH amplification
ClampFISH
Transcription site
Transcriptional burst
Chromatin conformation
Online Access:https://doi.org/10.1186/s12864-025-11818-0
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