Roles of Whole-Genome Sequencing in Determining Transmission and Risk Factors Associated with Carbapenem-Resistant Organisms at a Vietnam HealthCare Center of Orphan Children
Antimicrobial resistance is one of the most concerning public health issues in Vietnam due to antibiotic-resistant Gram-negative bacteria, including carbapenem-resistant organisms (CROs), which might appear in remote areas in Vietnam. At this center, we performed a cross-sectional study and collecte...
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2025-01-01
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| author | Van Kim Nguyen Pirom Noisumdaeng Katiya Ivanovitch Eugene Athan Larry Croft Phuong Toai Nguyen Huu Phuc Pham Nguyen |
| author_facet | Van Kim Nguyen Pirom Noisumdaeng Katiya Ivanovitch Eugene Athan Larry Croft Phuong Toai Nguyen Huu Phuc Pham Nguyen |
| author_sort | Van Kim Nguyen |
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| description | Antimicrobial resistance is one of the most concerning public health issues in Vietnam due to antibiotic-resistant Gram-negative bacteria, including carbapenem-resistant organisms (CROs), which might appear in remote areas in Vietnam. At this center, we performed a cross-sectional study and collected fecal samples from humans (20 healthcare workers (HCWs) and 67 residents) and 175 environmental samples, with rectal and environment swabs, as data for this study, from September 2022 to December 2022. We conducted microbiological testing, including the MELAB Chromogenic CARBA agar plates, blood agar plates, and the BD PhoenixTM Automated Microbiology System to screen, isolate, and identify bacterial species and phenotypic tests for the antimicrobial susceptibility of carbapenem-resistant bacteria and the whole-genome sequencing for 12 CROs chosen to confirm the CRO transmission between HCWs and residents. The study findings showed that the prevalence and risk factors associated with CRO colonization were detected in HCWs, residents, and the environment in the Center of Care and Protection of Orphan Children. CRO transmission happened between HCWs and residents detected with WGS analysis. Whole-genome sequencing (WGS) plays a significant role in CRO control and prevention and reduces CRO transmission/colonization in this center and other healthcare settings. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Microbiology Research |
| spelling | doaj-art-d5e1249acf04426b839bdfda645761f52025-01-24T13:41:47ZengMDPI AGMicrobiology Research2036-74812025-01-011612810.3390/microbiolres16010028Roles of Whole-Genome Sequencing in Determining Transmission and Risk Factors Associated with Carbapenem-Resistant Organisms at a Vietnam HealthCare Center of Orphan ChildrenVan Kim Nguyen0Pirom Noisumdaeng1Katiya Ivanovitch2Eugene Athan3Larry Croft4Phuong Toai Nguyen5Huu Phuc Pham Nguyen6Faculty of Public Health, Thammasat University, Pathum Thani 12121, ThailandFaculty of Public Health, Thammasat University, Pathum Thani 12121, ThailandFaculty of Public Health, Thammasat University, Pathum Thani 12121, ThailandBarwon South West Public Health Unit, Bellerine St, Geelong, VIC 3220, AustraliaCharles River Laboratories, Microbial Solutions, Melbourne, VIC 3137, AustraliaFaculty of Public Health, Can Tho University of Medicine and Pharmacy, Can Tho City 722700, VietnamLaboratory Department, Hung Vuong Hospital, Ho Chi Minh 749000, VietnamAntimicrobial resistance is one of the most concerning public health issues in Vietnam due to antibiotic-resistant Gram-negative bacteria, including carbapenem-resistant organisms (CROs), which might appear in remote areas in Vietnam. At this center, we performed a cross-sectional study and collected fecal samples from humans (20 healthcare workers (HCWs) and 67 residents) and 175 environmental samples, with rectal and environment swabs, as data for this study, from September 2022 to December 2022. We conducted microbiological testing, including the MELAB Chromogenic CARBA agar plates, blood agar plates, and the BD PhoenixTM Automated Microbiology System to screen, isolate, and identify bacterial species and phenotypic tests for the antimicrobial susceptibility of carbapenem-resistant bacteria and the whole-genome sequencing for 12 CROs chosen to confirm the CRO transmission between HCWs and residents. The study findings showed that the prevalence and risk factors associated with CRO colonization were detected in HCWs, residents, and the environment in the Center of Care and Protection of Orphan Children. CRO transmission happened between HCWs and residents detected with WGS analysis. Whole-genome sequencing (WGS) plays a significant role in CRO control and prevention and reduces CRO transmission/colonization in this center and other healthcare settings.https://www.mdpi.com/2036-7481/16/1/28risk factorscarbapenem-resistant organisms (CROs)healthcare workersresidentswhole-genome sequencing |
| spellingShingle | Van Kim Nguyen Pirom Noisumdaeng Katiya Ivanovitch Eugene Athan Larry Croft Phuong Toai Nguyen Huu Phuc Pham Nguyen Roles of Whole-Genome Sequencing in Determining Transmission and Risk Factors Associated with Carbapenem-Resistant Organisms at a Vietnam HealthCare Center of Orphan Children Microbiology Research risk factors carbapenem-resistant organisms (CROs) healthcare workers residents whole-genome sequencing |
| title | Roles of Whole-Genome Sequencing in Determining Transmission and Risk Factors Associated with Carbapenem-Resistant Organisms at a Vietnam HealthCare Center of Orphan Children |
| title_full | Roles of Whole-Genome Sequencing in Determining Transmission and Risk Factors Associated with Carbapenem-Resistant Organisms at a Vietnam HealthCare Center of Orphan Children |
| title_fullStr | Roles of Whole-Genome Sequencing in Determining Transmission and Risk Factors Associated with Carbapenem-Resistant Organisms at a Vietnam HealthCare Center of Orphan Children |
| title_full_unstemmed | Roles of Whole-Genome Sequencing in Determining Transmission and Risk Factors Associated with Carbapenem-Resistant Organisms at a Vietnam HealthCare Center of Orphan Children |
| title_short | Roles of Whole-Genome Sequencing in Determining Transmission and Risk Factors Associated with Carbapenem-Resistant Organisms at a Vietnam HealthCare Center of Orphan Children |
| title_sort | roles of whole genome sequencing in determining transmission and risk factors associated with carbapenem resistant organisms at a vietnam healthcare center of orphan children |
| topic | risk factors carbapenem-resistant organisms (CROs) healthcare workers residents whole-genome sequencing |
| url | https://www.mdpi.com/2036-7481/16/1/28 |
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