Angularly Resolved Deep Brain Fluorescence Imaging Using a Single Optical Fiber
In this article, we report the development of a minimally invasive fiber optic based fluorescence probe which can reach deep brain objects and measure the intensity and spatial distribution of fluorescence signals in the tissue. In this design, the brain is scanned by a single penetrating side-firin...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Optics |
| Online Access: | http://dx.doi.org/10.1155/2018/8903413 |
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| author | Israel J. De La Rosa Mehdi Azimipour Patrick K. Cullen Fred J. Helmstetter Ramin Pashaie |
| author_facet | Israel J. De La Rosa Mehdi Azimipour Patrick K. Cullen Fred J. Helmstetter Ramin Pashaie |
| author_sort | Israel J. De La Rosa |
| collection | DOAJ |
| description | In this article, we report the development of a minimally invasive fiber optic based fluorescence probe which can reach deep brain objects and measure the intensity and spatial distribution of fluorescence signals in the tissue. In this design, the brain is scanned by a single penetrating side-firing optical fiber which delivers excitation light pulses to the tissue at different depths and orientations and simultaneously collects samples of fluorescence emission signals. Signal-to-noise ratio of the measurements is improved by adapting the pulse compression technique and the theory of optimal filters. Effects of each design parameter on the overall performance of the scanner, including the spatial resolution and speed of scanning, are analyzed and experimentally measured. In vivo experiments show that the new device, despite the simplicity of the design, provides valuable information particularly useful in optogenetic stimulation experiments where the exact position of the fiber tip and the radiation orientation can change the outcome of a test. |
| format | Article |
| id | doaj-art-d5c91037cf8f49adac4117290ca3c363 |
| institution | Kabale University |
| issn | 1687-9384 1687-9392 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Optics |
| spelling | doaj-art-d5c91037cf8f49adac4117290ca3c3632025-02-03T06:08:05ZengWileyInternational Journal of Optics1687-93841687-93922018-01-01201810.1155/2018/89034138903413Angularly Resolved Deep Brain Fluorescence Imaging Using a Single Optical FiberIsrael J. De La Rosa0Mehdi Azimipour1Patrick K. Cullen2Fred J. Helmstetter3Ramin Pashaie4Electrical and Computer Engineering Department, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin, 53201, USAVision Science and Advanced Retinal Imaging Laboratory (VSRI), UC Davis Eye Centre, Sacramento, California, 95817, USADepartment of Psychology, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin 53201, USADepartment of Psychology, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin 53201, USAElectrical and Computer Engineering Department, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin, 53201, USAIn this article, we report the development of a minimally invasive fiber optic based fluorescence probe which can reach deep brain objects and measure the intensity and spatial distribution of fluorescence signals in the tissue. In this design, the brain is scanned by a single penetrating side-firing optical fiber which delivers excitation light pulses to the tissue at different depths and orientations and simultaneously collects samples of fluorescence emission signals. Signal-to-noise ratio of the measurements is improved by adapting the pulse compression technique and the theory of optimal filters. Effects of each design parameter on the overall performance of the scanner, including the spatial resolution and speed of scanning, are analyzed and experimentally measured. In vivo experiments show that the new device, despite the simplicity of the design, provides valuable information particularly useful in optogenetic stimulation experiments where the exact position of the fiber tip and the radiation orientation can change the outcome of a test.http://dx.doi.org/10.1155/2018/8903413 |
| spellingShingle | Israel J. De La Rosa Mehdi Azimipour Patrick K. Cullen Fred J. Helmstetter Ramin Pashaie Angularly Resolved Deep Brain Fluorescence Imaging Using a Single Optical Fiber International Journal of Optics |
| title | Angularly Resolved Deep Brain Fluorescence Imaging Using a Single Optical Fiber |
| title_full | Angularly Resolved Deep Brain Fluorescence Imaging Using a Single Optical Fiber |
| title_fullStr | Angularly Resolved Deep Brain Fluorescence Imaging Using a Single Optical Fiber |
| title_full_unstemmed | Angularly Resolved Deep Brain Fluorescence Imaging Using a Single Optical Fiber |
| title_short | Angularly Resolved Deep Brain Fluorescence Imaging Using a Single Optical Fiber |
| title_sort | angularly resolved deep brain fluorescence imaging using a single optical fiber |
| url | http://dx.doi.org/10.1155/2018/8903413 |
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