Dynamic hierarchical ligand anisotropy for competing macrophage regulation in vivo
Diverse connective tissues exhibit hierarchical anisotropic structures that intricately regulate homeostasis and tissue functions for dynamic immune response modulation. In this study, remotely manipulable hierarchical nanostructures are tailored to exhibit multi-scale ligand anisotropy. Hierarchica...
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KeAi Communications Co., Ltd.
2025-05-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X2500009X |
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| author | Kanghyeon Kim Sunhong Min Ramar Thangam Kyong-Ryol Tag Hyun-Jeong Lee Jeongyun Heo Hwapyung Jung Thet Thet Swe Iman Zare Guosheng Song Alireza Hassani Najafabadi Junmin Lee Hyun-Do Jung Jong Seung Kim Sunghoon Hur Hyun-Cheol Song Sung-Gyu Park Kunyu Zhang Pengchao Zhao Liming Bian Se Hoon Kim Juyoung Yoon Jae-Pyoung Ahn Hong-Kyu Kim Heemin Kang |
| author_facet | Kanghyeon Kim Sunhong Min Ramar Thangam Kyong-Ryol Tag Hyun-Jeong Lee Jeongyun Heo Hwapyung Jung Thet Thet Swe Iman Zare Guosheng Song Alireza Hassani Najafabadi Junmin Lee Hyun-Do Jung Jong Seung Kim Sunghoon Hur Hyun-Cheol Song Sung-Gyu Park Kunyu Zhang Pengchao Zhao Liming Bian Se Hoon Kim Juyoung Yoon Jae-Pyoung Ahn Hong-Kyu Kim Heemin Kang |
| author_sort | Kanghyeon Kim |
| collection | DOAJ |
| description | Diverse connective tissues exhibit hierarchical anisotropic structures that intricately regulate homeostasis and tissue functions for dynamic immune response modulation. In this study, remotely manipulable hierarchical nanostructures are tailored to exhibit multi-scale ligand anisotropy. Hierarchical nanostructure construction involves coupling liganded nanoscale isotropic/anisotropic Au (comparable to few integrin molecules-scale) to the surface of microscale isotropic/anisotropic magnetic Fe3O4 (comparable to integrin cluster-scale) and then elastically tethering them to a substrate. Systematic independent tailoring of nanoscale or microscale ligand isotropy versus anisotropy in four different hierarchical nanostructures with constant liganded surface area demonstrates similar levels of integrin molecule bridging and macrophage adhesion on the nanoscale ligand isotropy versus anisotropy. Conversely, the levels of integrin cluster bridging across hierarchical nanostructures and macrophage adhesion are significantly promoted by microscale ligand anisotropy compared with microscale ligand isotropy. Furthermore, microscale ligand anisotropy dominantly activates the host macrophage adhesion and pro-regenerative M2 polarization in vivo over the nanoscale ligand anisotropy, which can be cyclically reversed by substrate-proximate versus substrate-distant magnetic manipulation. This unprecedented scale-specific regulation of cells can be diversified by unlimited tuning of the scale, anisotropy, dimension, shape, and magnetism of hierarchical structures to decipher scale-specific dynamic cell-material interactions to advance immunoengineering strategies. |
| format | Article |
| id | doaj-art-d5be1edc8754485fa476fd9201f605b3 |
| institution | Kabale University |
| issn | 2452-199X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj-art-d5be1edc8754485fa476fd9201f605b32025-01-21T04:13:16ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2025-05-0147121135Dynamic hierarchical ligand anisotropy for competing macrophage regulation in vivoKanghyeon Kim0Sunhong Min1Ramar Thangam2Kyong-Ryol Tag3Hyun-Jeong Lee4Jeongyun Heo5Hwapyung Jung6Thet Thet Swe7Iman Zare8Guosheng Song9Alireza Hassani Najafabadi10Junmin Lee11Hyun-Do Jung12Jong Seung Kim13Sunghoon Hur14Hyun-Cheol Song15Sung-Gyu Park16Kunyu Zhang17Pengchao Zhao18Liming Bian19Se Hoon Kim20Juyoung Yoon21Jae-Pyoung Ahn22Hong-Kyu Kim23Heemin Kang24Department of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of KoreaDepartment of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of KoreaDepartment of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of KoreaDepartment of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of Korea; Advanced Analysis Center, Korea Institute of Science and Technology (KIST), Seoul, 02792, Republic of KoreaDepartment of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of Korea; Advanced Analysis Center, Korea Institute of Science and Technology (KIST), Seoul, 02792, Republic of KoreaCenter for Theragnosis, Korea Institute of Science and Technology (KIST), Seoul, 02792, Republic of KoreaDepartment of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of KoreaDepartment of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of KoreaResearch and Development Department, Sina Medical Biochemistry Technologies Co., Ltd., Shiraz, 7178795844, IranState Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, ChinaTerasaki Institute for Biomedical Innovation, Los Angeles, CA, 90064, USADepartment of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH), Pohang, 790-784, Republic of KoreaDivision of Materials Science and Engineering, Hanyang University, Seoul, 04763, Republic of KoreaDepartment of Chemistry, Korea University, Seoul, 02841, Republic of KoreaElectronic Materials Research Center, Korea Institute of Science and Technology (KIST), Seoul, 02792, Republic of Korea; KHU-KIST Department of Converging Science and Technology, Kyung Hee University, Yongin, 17104, Republic of KoreaElectronic Materials Research Center, Korea Institute of Science and Technology (KIST), Seoul, 02792, Republic of Korea; School of Advanced Materials Science and Engineering, Sungkyunkwan University (SKKU), Suwon, 16419, Republic of Korea; KIST-SKKU Carbon-Neutral Research Center, Sungkyunkwan University (SKKU), Suwon, 16419, Republic of KoreaDepartment of Nano-Bio Convergence, Korea Institute of Materials Science (KIMS), Changwon, Gyeongnam, 51508, Republic of Korea; Department of Future Convergence Materials, Korea University, Seoul, 02841, Republic of KoreaSchool of Biomedical Sciences and Engineering, Guangzhou International Campus, South China University of Technology, Guangzhou, 511442, ChinaSchool of Biomedical Sciences and Engineering, Guangzhou International Campus, South China University of Technology, Guangzhou, 511442, ChinaSchool of Biomedical Sciences and Engineering, Guangzhou International Campus, South China University of Technology, Guangzhou, 511442, ChinaCenter for Theragnosis, Korea Institute of Science and Technology (KIST), Seoul, 02792, Republic of Korea; KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, 02841, Republic of KoreaDepartment of Chemistry and Nanoscience, Ewha Womans University, Seoul, 03760, Republic of Korea; Graduate Program in Innovative Biomaterials Convergence, Ewha Womans University, Seoul, 03760, Republic of KoreaAdvanced Analysis Center, Korea Institute of Science and Technology (KIST), Seoul, 02792, Republic of KoreaAdvanced Analysis Center, Korea Institute of Science and Technology (KIST), Seoul, 02792, Republic of Korea; Corresponding author. Advanced Analysis Center, Korea Institute of Science and Technology (KIST), Seoul, 02792, Republic of Korea.Department of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of Korea; Department of Future Convergence Materials, Korea University, Seoul, 02841, Republic of Korea; College of Medicine, Korea University, Seoul, 02841, Republic of Korea; Corresponding author. Department of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of Korea.Diverse connective tissues exhibit hierarchical anisotropic structures that intricately regulate homeostasis and tissue functions for dynamic immune response modulation. In this study, remotely manipulable hierarchical nanostructures are tailored to exhibit multi-scale ligand anisotropy. Hierarchical nanostructure construction involves coupling liganded nanoscale isotropic/anisotropic Au (comparable to few integrin molecules-scale) to the surface of microscale isotropic/anisotropic magnetic Fe3O4 (comparable to integrin cluster-scale) and then elastically tethering them to a substrate. Systematic independent tailoring of nanoscale or microscale ligand isotropy versus anisotropy in four different hierarchical nanostructures with constant liganded surface area demonstrates similar levels of integrin molecule bridging and macrophage adhesion on the nanoscale ligand isotropy versus anisotropy. Conversely, the levels of integrin cluster bridging across hierarchical nanostructures and macrophage adhesion are significantly promoted by microscale ligand anisotropy compared with microscale ligand isotropy. Furthermore, microscale ligand anisotropy dominantly activates the host macrophage adhesion and pro-regenerative M2 polarization in vivo over the nanoscale ligand anisotropy, which can be cyclically reversed by substrate-proximate versus substrate-distant magnetic manipulation. This unprecedented scale-specific regulation of cells can be diversified by unlimited tuning of the scale, anisotropy, dimension, shape, and magnetism of hierarchical structures to decipher scale-specific dynamic cell-material interactions to advance immunoengineering strategies.http://www.sciencedirect.com/science/article/pii/S2452199X2500009XHierarchical ligand nanostructureMulti-scale ligand anisotropyRemote manipulationReversible macrophage regulation |
| spellingShingle | Kanghyeon Kim Sunhong Min Ramar Thangam Kyong-Ryol Tag Hyun-Jeong Lee Jeongyun Heo Hwapyung Jung Thet Thet Swe Iman Zare Guosheng Song Alireza Hassani Najafabadi Junmin Lee Hyun-Do Jung Jong Seung Kim Sunghoon Hur Hyun-Cheol Song Sung-Gyu Park Kunyu Zhang Pengchao Zhao Liming Bian Se Hoon Kim Juyoung Yoon Jae-Pyoung Ahn Hong-Kyu Kim Heemin Kang Dynamic hierarchical ligand anisotropy for competing macrophage regulation in vivo Bioactive Materials Hierarchical ligand nanostructure Multi-scale ligand anisotropy Remote manipulation Reversible macrophage regulation |
| title | Dynamic hierarchical ligand anisotropy for competing macrophage regulation in vivo |
| title_full | Dynamic hierarchical ligand anisotropy for competing macrophage regulation in vivo |
| title_fullStr | Dynamic hierarchical ligand anisotropy for competing macrophage regulation in vivo |
| title_full_unstemmed | Dynamic hierarchical ligand anisotropy for competing macrophage regulation in vivo |
| title_short | Dynamic hierarchical ligand anisotropy for competing macrophage regulation in vivo |
| title_sort | dynamic hierarchical ligand anisotropy for competing macrophage regulation in vivo |
| topic | Hierarchical ligand nanostructure Multi-scale ligand anisotropy Remote manipulation Reversible macrophage regulation |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X2500009X |
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