Emergence and polyclonal dissemination of NDM-5/OXA-181 carbapenemase-producing Escherichia coli in the French Indian Ocean territories
Abstract Aim Located in the Southwest Indian Ocean area (SIOA), the two French overseas territories (FOTs) of Reunion and Mayotte islands are heavily impacted by antimicrobial resistance. The aim of this study was to investigate all cases of NDM-5 and OXA-181 carbapenemase-producing Escherichia coli...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-01-01
|
| Series: | Annals of Clinical Microbiology and Antimicrobials |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12941-025-00778-8 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832571960324784128 |
|---|---|
| author | Thibaut Vedani Matthieu Pot Thomas Garrigos Loïk Sababadichetty Marion Daniel David Wilkinson Thierry Benoit-Cattin Olivier Belmonte Patrick Mavingui Laurent Dortet Guillaume Miltgen |
| author_facet | Thibaut Vedani Matthieu Pot Thomas Garrigos Loïk Sababadichetty Marion Daniel David Wilkinson Thierry Benoit-Cattin Olivier Belmonte Patrick Mavingui Laurent Dortet Guillaume Miltgen |
| author_sort | Thibaut Vedani |
| collection | DOAJ |
| description | Abstract Aim Located in the Southwest Indian Ocean area (SIOA), the two French overseas territories (FOTs) of Reunion and Mayotte islands are heavily impacted by antimicrobial resistance. The aim of this study was to investigate all cases of NDM-5 and OXA-181 carbapenemase-producing Escherichia coli (CPEc) in these two FOTs between 2015 and 2020, to better understand the regional spread of these last-line treatment resistant bacteria. Methods All E. coli isolates not susceptible to ertapenem from various public and private hospitals on Reunion and Mayotte islands were screened for carbapenemase production. Clinical and microbiological data were collected for each case. Genotypic analysis of the isolates was carried out using WGS to determine the clonality relationship between the isolates and the genetic support of the carbapenemase-encoding genes. Results A total of 92 isolates of NDM-5 (n = 67) and OXA-181 (n = 25) CPEc was collected from Reunion (n = 55) and Mayotte (n = 37) islands. Whole-genome sequencing identified 4 majors STs (ST58, ST167, ST405 and ST410). Genotypic analysis demonstrated numerous intra-ST possible cross transmission events, including strains isolated in both islands. Finally, all isolates (100%) carried the bla NDM-5 or bla OXA-181 genes on plasmids (IncF2, IncX3), most of which were conserved and identified in various STs. Conclusion We highlighted the dual dissemination of successful plasmids and the worrying circulation of high-risk clones via patients transfer between these two FOTs. It is therefore essential to effectively screen these patients for CPEc carriage on admission and to take these plasmids into account when investigating intra- or inter-hospital CPEc outbreaks. |
| format | Article |
| id | doaj-art-d5b7dec85c444e5993006af67023112d |
| institution | Kabale University |
| issn | 1476-0711 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Annals of Clinical Microbiology and Antimicrobials |
| spelling | doaj-art-d5b7dec85c444e5993006af67023112d2025-02-02T12:12:28ZengBMCAnnals of Clinical Microbiology and Antimicrobials1476-07112025-01-012411710.1186/s12941-025-00778-8Emergence and polyclonal dissemination of NDM-5/OXA-181 carbapenemase-producing Escherichia coli in the French Indian Ocean territoriesThibaut Vedani0Matthieu Pot1Thomas Garrigos2Loïk Sababadichetty3Marion Daniel4David Wilkinson5Thierry Benoit-Cattin6Olivier Belmonte7Patrick Mavingui8Laurent Dortet9Guillaume Miltgen10Laboratoire de Bactériologie, CHU Félix GuyonUMR Processus Infectieux en Milieu Insulaire Tropical (PIMIT), CNRS 9192, INSERM U1187, IRD 249, Université de La RéunionLaboratoire de Bactériologie, CHU Félix GuyonUMR Processus Infectieux en Milieu Insulaire Tropical (PIMIT), CNRS 9192, INSERM U1187, IRD 249, Université de La RéunionUMR Processus Infectieux en Milieu Insulaire Tropical (PIMIT), CNRS 9192, INSERM U1187, IRD 249, Université de La RéunionUMR Processus Infectieux en Milieu Insulaire Tropical (PIMIT), CNRS 9192, INSERM U1187, IRD 249, Université de La RéunionLaboratoire de Biologie, CH MayotteLaboratoire de Bactériologie, CHU Félix GuyonUMR Processus Infectieux en Milieu Insulaire Tropical (PIMIT), CNRS 9192, INSERM U1187, IRD 249, Université de La RéunionLaboratoire de Bactériologie, CHU de Bicêtre, Assistance Publique des Hôpitaux de Paris, Centre national de référence de la Résistance aux antibiotiques, laboratoire associé “Entérobactéries productrices de carbapénèmases”Laboratoire de Bactériologie, CHU Félix GuyonAbstract Aim Located in the Southwest Indian Ocean area (SIOA), the two French overseas territories (FOTs) of Reunion and Mayotte islands are heavily impacted by antimicrobial resistance. The aim of this study was to investigate all cases of NDM-5 and OXA-181 carbapenemase-producing Escherichia coli (CPEc) in these two FOTs between 2015 and 2020, to better understand the regional spread of these last-line treatment resistant bacteria. Methods All E. coli isolates not susceptible to ertapenem from various public and private hospitals on Reunion and Mayotte islands were screened for carbapenemase production. Clinical and microbiological data were collected for each case. Genotypic analysis of the isolates was carried out using WGS to determine the clonality relationship between the isolates and the genetic support of the carbapenemase-encoding genes. Results A total of 92 isolates of NDM-5 (n = 67) and OXA-181 (n = 25) CPEc was collected from Reunion (n = 55) and Mayotte (n = 37) islands. Whole-genome sequencing identified 4 majors STs (ST58, ST167, ST405 and ST410). Genotypic analysis demonstrated numerous intra-ST possible cross transmission events, including strains isolated in both islands. Finally, all isolates (100%) carried the bla NDM-5 or bla OXA-181 genes on plasmids (IncF2, IncX3), most of which were conserved and identified in various STs. Conclusion We highlighted the dual dissemination of successful plasmids and the worrying circulation of high-risk clones via patients transfer between these two FOTs. It is therefore essential to effectively screen these patients for CPEc carriage on admission and to take these plasmids into account when investigating intra- or inter-hospital CPEc outbreaks.https://doi.org/10.1186/s12941-025-00778-8EmergencePolyclonal disseminationNDM-5OXA-181CarbapenemasesEscherichia coli |
| spellingShingle | Thibaut Vedani Matthieu Pot Thomas Garrigos Loïk Sababadichetty Marion Daniel David Wilkinson Thierry Benoit-Cattin Olivier Belmonte Patrick Mavingui Laurent Dortet Guillaume Miltgen Emergence and polyclonal dissemination of NDM-5/OXA-181 carbapenemase-producing Escherichia coli in the French Indian Ocean territories Annals of Clinical Microbiology and Antimicrobials Emergence Polyclonal dissemination NDM-5 OXA-181 Carbapenemases Escherichia coli |
| title | Emergence and polyclonal dissemination of NDM-5/OXA-181 carbapenemase-producing Escherichia coli in the French Indian Ocean territories |
| title_full | Emergence and polyclonal dissemination of NDM-5/OXA-181 carbapenemase-producing Escherichia coli in the French Indian Ocean territories |
| title_fullStr | Emergence and polyclonal dissemination of NDM-5/OXA-181 carbapenemase-producing Escherichia coli in the French Indian Ocean territories |
| title_full_unstemmed | Emergence and polyclonal dissemination of NDM-5/OXA-181 carbapenemase-producing Escherichia coli in the French Indian Ocean territories |
| title_short | Emergence and polyclonal dissemination of NDM-5/OXA-181 carbapenemase-producing Escherichia coli in the French Indian Ocean territories |
| title_sort | emergence and polyclonal dissemination of ndm 5 oxa 181 carbapenemase producing escherichia coli in the french indian ocean territories |
| topic | Emergence Polyclonal dissemination NDM-5 OXA-181 Carbapenemases Escherichia coli |
| url | https://doi.org/10.1186/s12941-025-00778-8 |
| work_keys_str_mv | AT thibautvedani emergenceandpolyclonaldisseminationofndm5oxa181carbapenemaseproducingescherichiacoliinthefrenchindianoceanterritories AT matthieupot emergenceandpolyclonaldisseminationofndm5oxa181carbapenemaseproducingescherichiacoliinthefrenchindianoceanterritories AT thomasgarrigos emergenceandpolyclonaldisseminationofndm5oxa181carbapenemaseproducingescherichiacoliinthefrenchindianoceanterritories AT loiksababadichetty emergenceandpolyclonaldisseminationofndm5oxa181carbapenemaseproducingescherichiacoliinthefrenchindianoceanterritories AT mariondaniel emergenceandpolyclonaldisseminationofndm5oxa181carbapenemaseproducingescherichiacoliinthefrenchindianoceanterritories AT davidwilkinson emergenceandpolyclonaldisseminationofndm5oxa181carbapenemaseproducingescherichiacoliinthefrenchindianoceanterritories AT thierrybenoitcattin emergenceandpolyclonaldisseminationofndm5oxa181carbapenemaseproducingescherichiacoliinthefrenchindianoceanterritories AT olivierbelmonte emergenceandpolyclonaldisseminationofndm5oxa181carbapenemaseproducingescherichiacoliinthefrenchindianoceanterritories AT patrickmavingui emergenceandpolyclonaldisseminationofndm5oxa181carbapenemaseproducingescherichiacoliinthefrenchindianoceanterritories AT laurentdortet emergenceandpolyclonaldisseminationofndm5oxa181carbapenemaseproducingescherichiacoliinthefrenchindianoceanterritories AT guillaumemiltgen emergenceandpolyclonaldisseminationofndm5oxa181carbapenemaseproducingescherichiacoliinthefrenchindianoceanterritories |